An international survey about nail histology processing techniques

Abstract

Nail histopathology processing has some small variations from normal skin processing.

Nail clipping with onychomycosis and surprise clue to the diagnosis of nail unit melanoma

“…Ltd.) as softening agent since the immunohistochemical profile is not altered. 16,17 Fontana-Masson stain can help assess the presence of melanin pigment within the plate, but careful evaluation of tissue sections FIGURE 1 Yellow discoloration of the nail plate and linear spike of xanthonychia consistent with dermatophytoma FIGURE 2 High-power view of nail clipping. Clear spaces are seen at different levels of the nail plate, some have retained nuclei (black arrows).…”

“…Some authors favor the use of Mollifex Gurr (VWR Intl. Ltd.) as softening agent since the immunohistochemical profile is not altered . Fontana‐Masson stain can help assess the presence of melanin pigment within the plate, but careful evaluation of tissue sections is encouraged, given frequently confounding artifacts.…”

Retrospektive monozentrische 8-Jahres-Analyse von Nagelbiopsien zur Korrelation klinischer und histologischer Daten

“…Biopsie/Operation an nicht repräsentativer Lokalisation. Die histopathologische Prozessierung von Nagelbiopsien erfordert Variationen verglichen mit der Prozessie-rung kutaner Proben [19]. Bspw.…”

“…Dies vereinfacht die weitere Prozessierung erheblich. Weitere Unterschiede bestehen in der Schnittdicke der Präparate, diese variierte in der Studie von Wlodek et al von 4 -9 µm [19].…”

Nail histology processing techniques: What is happening around the world

“…In this issue of the Journal of Cutaneous Pathology , Wlodek et al present data from an international survey of members of the Council for Nail Disorders and the European Nail Society regarding nail histology processing techniques. This is an important contribution to the nail pathology literature as few existing publications address the topics investigated by the survey.…”