An International Collaborative Study of Renin Assay: Establishment of the International Reference Preparation of Human Renin

Bangham, D. R.; Robertson, Iris; Robertson, J. I. S.; Robinson, C. J.

doi:10.1042/cs048135s

Cited by 47 publications

(38 citation statements)

References 8 publications

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“…However, when these rates of angiotensin formation were calibrated against the Renin Standard and expressed as renin concentration in international units, the results agreed remarkably well. 4 6. Angiotensin was produced from dog substrate by each of the seven standard renin preparations with a mean rate of 55 X 10* ng angiotensin/unit renin/16 hrs (table 4).…”

Section: Discussionmentioning

confidence: 99%

“…The substrate from dog serum, however, reacts rapidly with the renin of all seven species investigated and was thus well qualified for their assay (tables 3 and 4). 4. Uniform rates of angiotensin formation were obtained (table 4) from six batches of renin substrate that had been prepared by three methods from the serum of six nephrectomized dogs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Micromethod for the assay of renin of seven species.

Haas¹,

Lewis²,

Scipione³

et al. 1979

Hypertension

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SUMMARY Renin was extracted and purified from human, dog, bog, rat, beef, rabbit and sheep kidneys. The renin "concentration" of these preparations was determined and expressed in international (Goldblatt) units by measuring the pressor effect produced by intravascular injection into normal dogs of a permanent colony. The renin "activity" was determined by bioassay, in the rat, of the angiotensin produced by incubation in vitro with renin substrate prepared from the serum of nephrectomized dogs.The rate of angiotensin formation was proportional to the concentration of renin, independent of the substrate concentration, and rather similar for renin of all seven species (mean rate =» 55 X 10 4 ng angiotensin/unit renin/16 hrs).Due to this uniformity, either of the two international reference preparations of renin (human or hog) from the World Health Organization can serve as an internal standard in the assay of renin of each of the seven species, to express their concentration in terms of the international unit.Renin substrate from hog plasma was suitable for the assay of human, dog and nog renin (mean rate = 55 X 10*). However, it reacted much more slowly with the renin of rat, beef, rabbit and sheep (mean rate = 9 X 10 Such test dogs, however, are usually not available in most laboratories and, furthermore, a relatively large dose, such as 1 unit of renin, would be required to raise the blood pressure of such a dog by 30 mm Hg.The present "indirect" assay procedure makes it possible to determine rather low concentrations; for example, 0.5 X 10~* unit of renin, the amount present in 0.005 mg of rabbit kidney. This procedure is based on the prolonged (16 hours at 38 °C) incubation of renin in vitro with its substrate and the subsequent assay of the angiotensin produced.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Micromethod for the assay of renin of seven species.

Haas¹,

Lewis²,

Scipione³

et al. 1979

Hypertension

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show abstract

“…12 Concentrations of human renin are presented in International Units (IU) referable to the WHO standard obtained from the National Institute of Biological Standards and Control, Holly Hill, London. 16 In a preliminary experiment, mouse renin was assayed by EKA using bi-nephrectomy rat plasma as the substrate, as previously described.…”

Section: Quantification Of Total and Active Renin And Proreninmentioning

confidence: 99%

Lectin chromatography of extrarenal renin protein in human plasma and tissues: Potential endocrine function via the renin receptor

Stubbs

Skinner

2004

J Renin Angiotensin Aldosterone Syst

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“…A long-standing and much repeated plea, [262][263][264][265] almost as often ignored, is for the general adoption of a renin standard, enabling results to be expressed in common, quantitative, terms. It is truly shameful that we remain largely unable to compare the results of renin assays from time to time, or from one laboratory to another.…”

Section: Standardisationmentioning

confidence: 99%