An interlaboratory study on the detection methods for enterotoxigenic Escherichia coli in vegetables using enterotoxin gene screening and selective agars for ETEC-specific isolation

Hara-Kudo, Yukiko; Ohtsuka, Kayoko; Konishi, Noriko; Yoshida, Takako; Iwabuchi, Kaori; Hiratsuka, Takahiro; Nagai, Yuhki; Kimata, Keiko; Wada, Hiroo; Yamazaki, Takumiko; Tsuchiya, Akihiko; Mori, Tetsuya; Inagaki, Shunichi; Shiraishi, Shogo; Terajima, Jun

doi:10.1016/j.ijfoodmicro.2020.108832

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…Because the main symptoms of patients were abdominal pain, diarrhoea, vomiting, and fever and the results of the test in Toyama City Public Health Center were referred, L. monocytogenes , S. aureus, B. cereus, E. albertii, and diarrhoeagenic E. coli were targeted to detect by enrichment and isolation as described in . In addition, E. albertii and diarrhoeagenic E. coli were tested by PCR; E. albertii- specific gene [ 6 ] for E. albertii ; stx [ 7 ] and eae [ 8 ] for enterohaemorrhagic E. coli (EHEC); heat-labile enterotoxin (LT) [ 9 ] and heat-stable toxin (ST) [ 9 ] for enterotoxigenic E. coli (ETEC); eae [ 8 ] and bfpA [ 10 ] for enteropathogenic E. coli (EPEC); aggR [ 11 ] for enteroaggregative E. coli (EAEC); astA [ 11 ] for enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1)-producing E. coli ; and invE (QuickPrimer InvE gene; Takara Co., Shiga, Japan) and ipaH (QuickPrimer IpaH gene; Takara Co.) for enteroinvasive E. coli (EIEC).…”

Section: Methodsmentioning

confidence: 99%

Atypical diarrhoeagenic Escherichia coli in milk related to a large foodborne outbreak

Hirose,

Ohya,

Yoshinari

et al. 2023

Epidemiol. Infect.

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A foodborne outbreak related to milk cartons served in school lunches occurred in June 2021, which involved more than 1,800 cases from 25 schools. The major symptoms were abdominal pain, diarrhoea, vomiting, and fever. Although major foodborne toxins and pathogens were not detected, a specific Escherichia coli strain, serotype OUT (OgGp9):H18, was predominantly isolated from milk samples related to the outbreak and most patients tested. The strains from milk and patient stool samples were identified as the same clone by core genome multilocus sequence typing and single-nucleotide polymorphism analysis. The strain was detected in milk samples served for two days related to the foodborne outbreak at a rate of 69.6% and levels of less than ten most probable number/100 mL but not on days unrelated to the outbreak. The acid tolerance of the strain for survival in the stomach was similar to that of enterohaemorrhagic E. coli O157:H7, and the same inserts in the chu gene cluster in the acid fitness island were genetically revealed. The pathogenicity of the strain was not clear; however, it was indicated that the causative pathogen was atypical diarrhoeagenic E. coli OUT (OgGp9):H18.

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