Previous researches demonstrated beneficial effects of the red (R) and blue (B) LED light for plant growth and development under the single format of the light spectrum. In these studies, the light spectra, intensities, and light hour controlled by growers in the growth chamber by supplying LED light with constant R/B ratio during the experimental period were indicated as important parameters. The purpose of this work is to investigate the effects of spectral distribution on the morphological growth of tomatoes seedling such as plant height, stem diameter, leaf area, leaf number, leaf thickness and leaf color, and to examine the effects of varying R/B LED light ratios between two periods of the young tomato. Tomatoes were soil-cultured with a 14-h photoperiod at 29/26℃, and 55%/75% relative humidity, under RB0.34+1.0 at 100-150 µmol m-2 s-1 (varying between two periods), commercial LED growth light (RB1.75) at 100 µmol m-2 s-1 (constant one period), and white LED at 100 µmol m-2 s-1 (as a control) inside growth chambers for 25 days. The analysis of variance statistic was applied to determine the mean difference of data. The results found that the tomato seedling under (RB0.34+1.0) has the best PAR spectrum to support the highest stem diameter, leaf thickness, and leaf color (highest chlorophyll content). Moreover, the tomato seedling grown under commercial LED light is also acceptable. The advantage of RB0.34+1.0 treatment is very good for promoting high quality tomato seedlings, perfect leaf and stem, for reducing the stem damage on the transplants, and increasing the tomato production.