An integrative understanding of the large metabolic shifts induced by antibiotics in critical illness

Marfil-Sánchez, Andrea; Zhang, Lu; Alonso-Pernas, Pol; Mirhakkak, Mohammad H.; Mueller, Melinda; Seelbinder, Bastian; Ni, Yueqiong; Santhanam, Rakesh; Busch, Anne; Beemelmanns, Christine; Ermolaeva, Maria A.; Bauer, Michael; Panagiotou, Gianni

doi:10.1080/19490976.2021.1993598

Cited by 10 publications

(5 citation statements)

References 123 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As it has been reported that aging is negatively correlated with the diversity of the gut microbiota [ 75 ]. Both the effects and mechanisms of antibiotic-driven gut microbiota disruption have been used as an important part of microbiology research in critically ill ICU patients [ 76 , 77 ]. Although there was no difference in antibiotic use between the two groups in this study, the effect of antibiotics on the microbiome cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

The gut-lung axis in critical illness: microbiome composition as a predictor of mortality at day 28 in mechanically ventilated patients

Zhou,

Zou,

et al. 2023

BMC Microbiol

View full text Add to dashboard Cite

Background Microbial communities are of critical importance in the human host. The lung and gut microbial communities represent the most essential microbiota within the human body, collectively referred to as the gut-lung axis. However, the differentiation between these communities and their influence on clinical outcomes in critically ill patients remains uncertain. Methods An observational cohort study was obtained in the intensive care unit (ICU) of an affiliated university hospital. Sequential samples were procured from two distinct anatomical sites, namely the respiratory and intestinal tracts, at two precisely defined time intervals: within 48 h and on day 7 following intubation. Subsequently, these samples underwent a comprehensive analysis to characterize microbial communities using 16S ribosomal RNA (rRNA) gene sequencing and to quantify concentrations of fecal short-chain fatty acids (SCFAs). The primary predictors in this investigation included lung and gut microbial diversity, along with indicator species. The primary outcome of interest was the survival status at 28 days following mechanical ventilation. Results Sixty-two mechanically ventilated critically ill patients were included in this study. Compared to the survivors, the diversity of microorganisms was significantly lower in the deceased, with a significant contribution from the gut-originated fraction of lung microorganisms. Lower concentrations of fecal SCFAs were detected in the deceased. Multivariate Cox regression analysis revealed that not only lung microbial diversity but also the abundance of Enterococcaceae from the gut were correlated with day 28 mortality. Conclusion Critically ill patients exhibited lung and gut microbial dysbiosis after mechanical ventilation, as evidenced by a significant decrease in lung microbial diversity and the proliferation of Enterococcaceae in the gut. Levels of fecal SCFAs in the deceased served as a marker of imbalance between commensal and pathogenic flora in the gut. These findings emphasize the clinical significance of microbial profiling in predicting the prognosis of ICU patients.

show abstract

Section: Discussionmentioning

confidence: 99%

The gut-lung axis in critical illness: microbiome composition as a predictor of mortality at day 28 in mechanically ventilated patients

Zhou,

Zou,

et al. 2023

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…On the contrary, the Lactobacillus_unclassified, Lactobacillus_hilgardii, Lactobacillus_reuteri, and Lactobacillus_vaginalis were significantly decreased in the CLP + BBR group. A study reported that Bifidobacterium animalis and Lactobacillus crispatus would promote Caenorhabditis Elegans expansion in ICU patients, revealing a significant negative impact of these microbes on host viability and developmental homeostasis ( 44 ). In contrast, Lactobacillu s _reuteri is believed to be able to antagonize neonatal sepsis ( 45 ).…”

Section: Discussionmentioning

confidence: 99%

Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis

et al. 2022

View full text Add to dashboard Cite

IntroductionSepsis, an infection with multiorgan dysfunction, is a serious burden on human health. Berberine (BBR), a bioactive component, has a protective effect on sepsis and the effect may be related to gut microbiota. However, studies on the role of BBR with gut microbiota in sepsis are lacking. Therefore, this study investigated the ameliorative effects and the underlying mechanisms of BBR on cecal ligature and puncture (CLP) rats.MethodsThis study has observed the effect of BBR on pathological injury, Inflammation, intestinal barrier function, gut microbiota, and metabolite change in CLP rats by Hematoxylin-eosin staining, enzyme-linked immunosorbent assays, flow cytometry, 16S rDNA, and metabolomics analyses.ResultsThe inhibition effects of BBR treatment on the histological damage of the lung, kidney, and ileum, the interleukin (IL)-1b, IL-6, IL-17A, and monocyte chemokine-1 levels in serum in CLP rats were proved. Also, the BBR inhibited the diamine-oxidase and fluorescein isothiocyanate-dextran 40 levels, suggesting it can improve intestinal barrier function disorders. The cluster of differentiation (CD) 4+, CD8+, and CD25+ Forkhead box protein P3 (Foxp3) + T lymphocytes in splenocytes were up-regulated by BBR, while the IL-17A+CD4+ cell level was decreased. The abundance of gut microbiota in CLP rats was significantly different from that of the sham and BBR treatment rats. The significantly changed metabolites in the serum mainly included carbohydrates, phenols, benzoic acids, alcohols, vitamins et al. Additionally, this study predicted that the biological mechanism of BBR to ameliorate sepsis involves glycolysis-, nucleotide-, and amino acid-related metabolic pathways.DiscussionThis study proved the strong correlation between the improvement effect of BBR on sepsis and gut microbiota and analyzed by metabolomics that gut microbiota may improve CLP rats through metabolites, providing a scientific basis for BBR to improve sepsis and a new direction for the study of the biological mechanism.

show abstract

“…To characterize the human gut fungal diversity and composition, we collected internal transcribed spacer (ITS) sequencing data from 15 published projects (Supplementary Table 1) (12,(19)(20)(21)(22)(23)(24)(25)(26)(27)(28). In addition, we recruited 572 Chinese participants (Chinese Gut Mycobiome cohort, or CHGM) aged from 17 to 89 years old and pro led their fecal mycobiome with ITS1 sequencing.…”

Section: Landscape Of Human Gut Mycobiome Composition and Diversitymentioning

confidence: 99%

Enterotypes of the human gut mycobiome

Lai

Yan

et al. 2023

Preprint

View full text Add to dashboard Cite

Background The fungal component of the human gut microbiome, also known as the mycobiome, plays a vital role in intestinal ecology and human health. However, the overall structure of the gut mycobiome as well as the inter-individual variations in fungal composition remains largely unknown. In this study, we collected a total of 3,363 fungal sequencing samples from 16 cohorts across three continents, including 572 newly profiled samples from China. Results We identify and characterize four mycobiome enterotypes using ITS profiling of 3,363 samples from 16 cohorts. These enterotypes exhibit stability across populations and geographical locations and significant correlation with bacterial enterotypes. Particularly, we notice that fungal enterotype have a strong age preference, where the enterotype dominated by Candida (i.e., fun_C_E enterotype) is enriched in the elderly populations and confers an increased risk of multiple diseases associated with compromised intestinal barrier. In addition, bidirectional mediation analysis reveals that the fungi-contributed aerobic respiration pathway associated with fun_C_E enterotype might mediate the association between the compromised intestinal barrier and aging. Conclusions We show that the human gut mycobiome is highly structured, and significantly correlates with prokaryotic community and multiple host factors such as diseases and host age.

show abstract

An integrative understanding of the large metabolic shifts induced by antibiotics in critical illness

Cited by 10 publications

References 123 publications

The gut-lung axis in critical illness: microbiome composition as a predictor of mortality at day 28 in mechanically ventilated patients

The gut-lung axis in critical illness: microbiome composition as a predictor of mortality at day 28 in mechanically ventilated patients

Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis

Enterotypes of the human gut mycobiome

Contact Info

Product

Resources

About