An integrative approach to improving the biocatalytic reactions of whole cells expressing recombinant enzymes

Shin, Kyung‐Chul; Oh, Deok‐Kun

doi:10.1007/s11274-021-03075-6

Cited by 1 publication

(2 citation statements)

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“…Biotransformation is a process of chemical modification in which a biocatalyst converts a raw chemical compound into a structurally related high-value-added product, and this process is often mediated by purified enzyme and whole cells [ 10 ]. Traditional chemical synthesis exhibits disadvantages over biotransformation, such as low specificity, harsh conditions, complex purification steps, and byproduct formation [ 11 ]. The enzyme-containing cells were harvested and used in the whole-cell biotransformation, which can eliminate the separation and purification process of the enzyme, thus reducing production costs.…”

Section: Introductionmentioning

confidence: 99%

“…Under the protection of the cell membrane, the structures of enzymes were more stable and the enzymes were more efficient [ 12 ]. Whole-cell biotransformation can also use cofactors that are already produced by the cells [ 11 ]. Due to its advantages, including mild reaction conditions, high titers, and ease of industrial and environmental protection, whole-cell bioconversion has become an attractive method of chemical production.…”

Section: Introductionmentioning

confidence: 99%

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Whole-Cell Biocatalytic Production of Acetoin with an aldC-Overexpressing Lactococcus lactis Using Soybean as Substrate

Luo

Liu

Luo

et al. 2023

Foods

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Douchi is a traditional Chinese fermented soybean product, in which acetoin is a key flavor substance. Here, the α-acetolactate decarboxylase gene aldC was cloned from Lactiplantibacillus (L.) plantarum and overexpressed in Lactococcus (L.) lactis NZ9000 by nisin induction. The ALDC crude enzyme solution produced an enzyme activity of 35.16 mU. Next, whole cells of the recombinant strain NZ9000/pNZ8048-aldC were employed as the catalyst to produce acetoin in GM17 medium. An optimization experiment showed that an initial OD600 of 0.6, initial pH of 7.5, nisin concentration of 20 ng/mL, induction temperature of 37 °C and static induction for 8 h were the optimal induction conditions, generating the maximum acetoin production (106.93 mg/L). Finally, after incubation under the optimal induction conditions, NZ9000/pNZ8048-aldC was used for whole-cell biocatalytic acetoin production, using soybean as the substrate. The maximum acetoin yield was 79.43 mg/L. To our knowledge, this is the first study in which the aldC gene is overexpressed in L. lactis and whole cells of the recombinant L. lactis are used as a biocatalyst to produce acetoin in soybean. Thus, our study provides a theoretical basis for the preparation of fermented foods containing high levels of acetoin and the biosynthesis of acetoin in food materials.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%