An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru

Gabrielson

et al. 2021

Journal of Phycology

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A partial rbcL sequence of the lectotype specimen of Corallina berteroi shows that it is the earliest available name for C. ferreyrae. Multilocus species delimitation analyses (ABGD, SPN, GMYC, bPTP, and BPP) using independent or concatenated COI, psbA, and rbcL sequences recognized one, two, or three species in this complex, but only with weak support for each species hypothesis. Conservatively, we recognize a single worldwide species in this complex of what appears to be multiple, evolving populations. Included in this species, besides C. ferreyrae, are C. caespitosa, the morphologically distinct C. melobesioides, and, based on a partial rbcL sequence of the holotype specimen, C. pinnatifolia. Corallina berteroi, not C. officinalis, is the cosmopolitan temperate species found thus far in the NE Atlantic, Mediterranean Sea, warm temperate NW Atlantic and NE Pacific, cold temperate SW Atlantic (Falkland Islands), cold and warm temperate SE Pacific, NW Pacific and southern Australia. Also proposed is C. yendoi sp. nov. from Hokkaido, Japan, which was recognized as distinct by 10 of the 13 species discrimination analyses, including the multilocus BPP.

Section: Methodsmentioning

confidence: 99%

Type specimen sequencing, multilocus analyses, and species delimitation methods recognize the cosmopolitan Corallina berteroi and establish the northern Japanese C. yendoi sp. nov. (Corallinaceae, Rhodophyta)

Gabrielson

et al. 2021

Journal of Phycology

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“…In the last decade, several of these species have been threatened by the high rate of deforestation, a serious concern that will eventually result in loss of biodiversity and uncontrolled genetic erosion of species with economic and ecological importance (Montesinos-Tub ee, 2020;Walker et al, 2021). This study highlights not only the importance of sequencing several molecular markers in applying and validating the names of Andean berries, but also the need to integrate morphological and DNA-based methods to understand the diversity along the Peruvian Andean cloud forest (Bustamante et al 2021(Bustamante et al , 2021(Bustamante et al , 2021Tineo et al, 2020). The characterization of berries biodiversity is an important element in any future strategy to develop ambitious commitments and tackle research, monitoring and protection programs across the Amazonas region (S anchez et al, 2021).…”

Section: Rubus Weberbauerimentioning

confidence: 84%

“…Genomic DNA was extracted from leaf tissue using the NucleoSpin Plant II Kit (Macherey-Nagel, Düren, Germany) following the Tineo et al (2020). Briefly, samples were homogenized in a freeze-crush apparatus (SK-100, Funakoshi, Japan).…”

Section: Dna Sequencing and Alignment Preparationmentioning

confidence: 99%

“…Each gene was amplified using polymerase chain reaction (PCR) with MasterMix (Promega, Wisconsin, USA) in the following reaction mixture: 10 ng of DNA and 0.25-0.5 pmol of forward and reverse primers for a total volume of 10 μl. The PCR protocols followed Bustamante et al (2021) and Tineo et al (2020), and primer combinations are summarized in Table 2. Amplicons were purified using the NucleoSpin ™ Gel and PCR Clean-up Kit protocol (Macherey-Nagel ™ , Düren, Germany).…”

Section: Dna Sequencing and Alignment Preparationmentioning

confidence: 99%

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Exploring the diversity of andean berries from northern Peru based on molecular analyses

Tineo

et al. 2022

Heliyon

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More than 12,000 species have been listed under the category of berries, and most of them belong to the orders Ericales and Rosales. Recent phylogenetic studies using molecular data have revealed disagreements with morphological approaches mainly due to diverse floral arrangements, which has proven to be a problem when recognizing species. Therefore, the use of multilocus sequence data is essential to establish robust species boundaries. Although berries are common in Andean cloud forests, diversity of these taxa has not been extensively evaluated in the current context of DNA-based techniques. In this regard, this study characterized morphologically and constructed multilocus phylogenies using four molecular markers, two chloroplast markers (matK and rbcL) and two nuclear markers (ITS and GBSSI-2). Specimens did not show diagnostic features to delimit species of berries. A total of 125 DNA-barcodes of andean berries were newly generated for the four molecular markers. The multilocus phylogenies constructed from these markers allowed the identification of 24 species grouped into the order Ericales (Cavendishia ¼ 1, Clethra ¼ 2, Disterigma ¼ 2, Gaultheria ¼ 4, Thibaudia ¼ 4, Vaccinium ¼ 3) and Rosales (Rubus ¼ 8), incorporating into the Peruvian flora four new records (Disterigma ecuadorense, Disterigma synanthum, Vaccinium meridionale and Rubus glabratus) and revealing the genus Rubus as the most diverse group of berries in the Amazonas region. The results of this study showed congruence in all the multilocus phylogenies, with internal transcribed spacer (ITS) showing the best resolution to distinguish the species. These species were found in coniferous forests, dry and humid forests, rocky slopes, and grasslands at 2,506-3,019 masl from Amazonas region. The integration of morphological and DNA-based methods is recommended to understand the diversity of berries along the Peruvian Andean cloud forest.

“…Vasconcellea carvalhoae is a newly described species of mountain papayas distributed in the wild in montane areas at 2236 m elevation in northeastern Peru (Tineo et al. 2020 ). This species is characterized by being dioecious trees to 4 m tall.…”

mentioning

confidence: 99%

Analysis of the complete plastidial genome of the newly highland papaya Vasconcellea carvalhoae (Caricaceae) from Peru

Tineo

Mitochondrial DNA Part B

2022

Especially in South American Andean communities, Vasconcellea carvalhoae D. Tineo & D.E. Bustamante 2020 is a significant highland papaya with agronomic promise. High-throughput sequencing of the holotype specimen of V. carvalhoae from Peru (KUELAP227) resulted in the assembly of its complete plastid genome (GenBank accession number ON764441). The plastid genome of this highland papaya is 158,723 bp and contains 130 genes. This plastid genome is similar in length, content, and organization to other members of Caricaceae, except for the absence of the pseudogene infA. Phylogenetic analyses of V. carvalhoae support its sistership to V. pubescens .