An integrative analysis of Qingfei Paidu Decoction for its anti-HCoV-229E mechanism in cold and damp environment based on the pharmacokinetics, metabolomics and molecular docking technology

Zhang, Yan; Gu, Xinru; Zhou, Yanyan; Si, Nan; Gao, Wenya; Sun, Bo; Sun, Jun; Li, Tao; Wang, Linna; Wei, Xiaolu; Guo, Shan; Cui, Xiaolan; Bian, Baolin; Wang, Hongjie; Wang, Liang; Zhao, Haiyu

doi:10.1016/j.phymed.2022.154527

Cited by 11 publications

(7 citation statements)

References 30 publications

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“…The active metabolites of Remdesivir and Molnupiravir selectively inhibit RdRp, which delays viral RNA chain termination and inhibits SARS-CoV-2 replication 24 . Previous studies revealed that many herbs and their bioactive ingredients could bind to ACE2, 3CLpro, and RdRp and inhibit their activities 486 , 487 . Compared with antiviral drug treatment alone, JHQG combined with antiviral drugs was superior in relieving fever and poor appetite in patients with COVID-19 488 .…”

Section: Potential Hdi Between Anti-covid-19 Drugs and The Tcm Recipesmentioning

confidence: 99%

“…A retrospective study showed that the combination of QFPD with antiviral drugs exerted significantly better anti-inflammatory effects compared with treatment with antiviral drugs alone in patients with mild and moderate COVID-19 69 . The active ingredients including glycyrrhizin, cinnamic acid, baicalin, and baicalein in QFPD can suppress SARS-CoV-2 replication via targeting 3CLpro and RdRp 362 , 486 , 489 . A meta-analysis indicated that LHQW combined with conventional therapy could effectively reduce the severity in patients with mild or moderate COVID-19 490 .…”

Section: Potential Hdi Between Anti-covid-19 Drugs and The Tcm Recipesmentioning

confidence: 99%

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Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

Ye¹,

Fan²,

Zhao³

et al. 2023

Acta Pharmaceutica Sinica B

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Section: Potential Hdi Between Anti-covid-19 Drugs and The Tcm Recipesmentioning

confidence: 99%

Section: Potential Hdi Between Anti-covid-19 Drugs and The Tcm Recipesmentioning

confidence: 99%

Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

Ye¹,

Fan²,

Zhao³

et al. 2023

Acta Pharmaceutica Sinica B

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“…QFPD potently inactivates CYP3A and substantially modulates the pharmacokinetics of CYP3A substrate drugs [86] . The comprehensive pharmacokinetic behavior of QFPD in vivo illustrates that QFPD exhibits therapeutic effect via immune regulation, anti-infection, anti-inflammatory activities, thereby ameliorating the symptoms of metabolic disorders [87] . QFPD improved immune function and reduced inflammation in a mouse model of pneumonia by remodeling the gut microbiota and host metabolism [88] .…”

Section: Mechanisms Through Which Tmtfs Regulate Immune and Inflammat...mentioning

confidence: 99%

“Three medicines and three formulas” in COVID-19: From bench to bedside

Lyu,

Xiao,

Wang

et al. 2023

Acupuncture and Herbal Medicine

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Chinese guideline has been proven effective in the fight against Coronavirus disease 2019 (COVID-19) during the epidemic spread globally. Traditional Chinese medicine (TCM) has been widely recognized for its effectiveness in alleviating symptoms, inhibiting disease deterioration, reducing mortality, and improving cure rate of COVID-19 patients. During the pandemic, “three medicines and three formulas” stood out from hundreds of registered clinical studies and became the highly recommended TCM for COVID-19 treatment. The “three medicines and three formulas” not only effectively relieve the clinical symptoms of fever, cough, fatigue, and phlegm, but also significantly shorten the time of nucleic acid negative conversion, improve lung computed tomography imaging feature and inflammation, ameliorate clinical biochemical indicators, and reduce sequelae. The potential pharmacological mechanisms of them are mainly relevant with the crosstalk of viral toxicity, endothelial damage, cytokine storm, immune response, and microthrombus. In brief, the clinical effects as well as the potential mechanisms of “three medicines and three formulas” on COVID-19 were systematically analyzed and summarized covering the whole stages of disease development, including virus invasion and replication, immune response and cytokine storm, and acute respiratory distress syndrome and multiple organ dysfunction syndrome. We hope that this review could provide theoretical basis and reference for in-depth understanding the positive role of “three medicines and three formulas” for COVID-19 treatment.

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“…As previously described ( 19 ), 50 μL plasma was obtained and put into a 0.5 mL centrifuge tube containing 200 μL cold acetonitrile. Then the samples were vortexed (3 min) and centrifuged (12000 rpm, 4 °C, 15 min) in turn.…”

Section: Untargeted Metabolomics Analysis Of Mice Plasmamentioning

confidence: 99%

“…Metabolic profiling of the mice’s lung tissue was performed with established methods ( 19 ). The UHPLC system (Ultimate 3000, Thermo Fisher Scientific, USA) with a UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm, Waters) was used to perform chromatographic separation at 35°C.…”

Section: Untargeted Metabolomics Analysis Of Mice Plasmamentioning

confidence: 99%

The immediate adverse drug reactions induced by ShenMai Injection are mediated by thymus-derived T cells and associated with RhoA/ROCK signaling pathway

et al. 2023

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IntroductionThe mechanism of the immediate adverse drug reactions (ADRs) induced by ShenMai injection (SMI) has not been completely elucidated. Within 30 minutes, the ears and lungs of mice injected with SMI for the first time showed edema and exudation reactions. These reactions were different from the IV hypersensitivity. The theory of pharmacological interaction with immune receptor (p-i) offered a new insight into the mechanisms of immediate ADRs induced by SMI.MethodsIn this study, we determined that the ADRs were mediated by thymus-derived T cells through the different reactions of BALB/c mice (thymus-derived T cell normal) and BALB/c nude mice (thymus-derived T cell deficient) after injecting SMI. The flow cytometric analysis, cytokine bead array (CBA) assay and untargeted metabolomics were used to explain the mechanisms of the immediate ADRs. Moreover, the activation of the RhoA/ROCK signaling pathway was detected by western blot analysis.ResultsIn BALB/c mice, the vascular leakage and histopathology results showed the occurrence of the immediate ADRs induced by SMI. The flow cytometric analysis revealed that CD4+ T cell subsets (Th1/Th2, Th17/Treg) were imbalanced. And the levels of cytokines such as IL-2, IL-4, IL12P70 and INF-γ increased significantly. However, in BALB/c nude mice, all the indicators mentioned above have not changed significantly. The metabolic profile of both BALB/c mice and BALB/c nude mice was significantly changed after injecting SMI, and the notable increase in lysolecithin level might have a greater association with the immediate ADRs induced by SMI. The Spearman correlation analysis revealed that LysoPC (18:3(6Z,9Z,12Z)/0:0) showed a significant positive correlation with cytokines. After injecting SMI, the levels of RhoA/ROCK signaling pathway-related protein increased significantly in BALB/c mice. Protein-protein interaction (PPI) showed that the increased lysolecithin levels might be related to the activation of the RhoA/ROCK signaling pathway.DiscussionTogether, the results of our study revealed that the immediate ADRs induced by SMI were mediated by thymus-derived T cells, and elucidated the mechanisms of such ADRs. This study provided new insights into the underlying mechanism of immediate ADRs induced by SMI.

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An integrative analysis of Qingfei Paidu Decoction for its anti-HCoV-229E mechanism in cold and damp environment based on the pharmacokinetics, metabolomics and molecular docking technology

Cited by 11 publications

References 30 publications

Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

“Three medicines and three formulas” in COVID-19: From bench to bedside

The immediate adverse drug reactions induced by ShenMai Injection are mediated by thymus-derived T cells and associated with RhoA/ROCK signaling pathway

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