An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells

Nassar, Farah; El, Rabab; Nasr, Rihab

doi:10.1007/978-1-4939-4011-0_18

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…More recently, a challenging hsa-mir183/EGR1/E2F1 axis has been reported to directly control CML stem cell behaviour 72. Most of these miRNAs have been extensively reviewed elsewhere 69,73–77…”

Section: Cooperating Oncogenesmentioning

confidence: 99%

<p>Strategies For Targeting Chronic Myeloid Leukaemia Stem Cells</p>

Carrà¹,

Cartellà²,

Maffeo³

et al. 2019

BLCTT

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Chronic Myeloid Leukaemia is a myeloproliferative disorder driven by the t(9;22) chromosomal translocation coding for the chimeric protein BCR-ABL. CML treatment represents the paradigm of molecular therapy of cancer. Since the development of the tyrosine kinase inhibitor of the BCR-ABL kinase, the clinical approach to CML has dramatically changed, with a stunning improvement in the quality of life and response rates of patients. However, it remains clear that tyrosine kinase inhibitors (TKIs) are unable to target the most immature cellular component of CML, the CML stem cell. This review summarizes new insights into the mechanisms of resistance to TKIs.

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Section: Cooperating Oncogenesmentioning

confidence: 99%

<p>Strategies For Targeting Chronic Myeloid Leukaemia Stem Cells</p>

Carrà¹,

Cartellà²,

Maffeo³

et al. 2019

BLCTT

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show abstract

“…By binding to the 3'-untranslated region (UTR) of target mRNA, miRNAs induce mRNA cleavage or translation inhibition (12). Each miRNA has multiple potential mRNA targets, and therefore, a broad-spectrum gene expression can be affected by an specific miRNA (13,14). Recent studies have demonstrated that miRNA inhibitory activity can be quantified by examining their target mRNA expression levels (15).…”

Section: Introductionmentioning

confidence: 99%

Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats

et al. 2019

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Inflammatory response has an important role in the outcome of cerebral ischemia reperfusion injury (CIR). Biliverdin (BV) administration can relieve CIR in rats, but the mechanism remains unknown. The aim of the present study was to explore the expressional network of microRNA (miRNA)-mRNA in CIR rats following BV administration. A rat middle cerebral artery occlusion model with BV treatment was established. After neurobehavior was evaluated by neurological severity scores (NSS), miRNA and mRNA expressional profiles were analyzed by microarray technology from the cerebral cortex subjected to ischemia and BV administration. Then, bioinformatics prediction was used to screen the correlation between miRNA and mRNA, and 20 candidate miRNAs and 33 candidate mRNAs were verified by reverse transcription-quantitative polymerase chain reaction. Furthermore, the regulation relationship between ETS proto-oncogene 1 (Ets1) and miRNA204-5p was examined by luciferase assay. A total of 86 miRNAs were differentially expressed in the BV group compared with the other groups. A total of 10 miRNAs and 26 candidate genes were identified as a core 'microRNA-mRNA' regulatory network that was linked with the functional improvement of BV administration in CIR rats. Lastly, the luciferase assay results confirmed that miRNA204-5p directly targeted Ets1. The present findings suggest that BV administration may regulate multiple miRNAs and mRNAs to improve neurobehavior in CIR rats, by influencing cell proliferation, apoptosis, maintaining ATP homeostasis, and angiogenesis.

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An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells

Cited by 2 publications

References 4 publications

<p>Strategies For Targeting Chronic Myeloid Leukaemia Stem Cells</p>

<p>Strategies For Targeting Chronic Myeloid Leukaemia Stem Cells</p>

Biliverdin administration regulates the microRNA-mRNA expressional network associated with neuroprotection in cerebral ischemia reperfusion injury in rats

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