An Integrated YAC Clone Contig for the WAGR Region on Human Chromosome 11p13–p14.1

Gawin, Beate; Klamt, B; König, A; Thäte, Claudia; Lepaslier, Denis; Chumakov, Ilya; Bhogal, R.; Zehetner, Günther; Bruns, G.; Gessler, Manfred

doi:10.1006/geno.1995.0006

Cited by 5 publications

(15 citation statements)

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“…Clone insert sizes were determined by NotI restriction digests of the respective PAC clones. Additionally, we used markers that were already integrated into a detailed physical map of 11p13 as hybridization probes (Gawin et al 1995). The combination of these data sets allowed an accurate alignment of the analyzed PAC clones along the physical map of 11p13.…”

Section: Contig Constructionmentioning

confidence: 99%

“…Genes are highlighted by shaded boxes and the newly integrated EST clones are written at a 45°angle. The positions of RAG1/2 and TRAF6 as well as of the three EST clones 60587, 125727, and 41188, have been determined by hybridization to YAC clones located at the centromeric border of 11p13 (Gawin et al 1995). (Aral et al 1997;Ling et al 1998).…”

Section: Identification Of 11p13 Specific Transcripts-gene Mapmentioning

confidence: 99%

“…1). Four of these 28 clones were located outside of the PAC contig to the centromeric border of 11p13, as they hybridized to YAC clones from a contig that extends further centromeric (Gawin et al 1995).…”

Section: Identification Of 11p13 Specific Transcripts-gene Mapmentioning

confidence: 99%

“…For each gene or EST cluster one representative hybridization probe was chosen to determine its corresponding position on the PAC contig map. For most of the known genes single copy probes (s.c.) have been used that are described in Gawin et al (1995). For G2, CSTF3 and the EST clusters, inserts of IMAGE clones have been employed.…”

Section: Expression Analyses With Mouse Cdna Clonesmentioning

confidence: 99%

“…Niederführ et al (1998) have constructed a 3 Mb sequence-ready PAC contig that covers the inner part of 11p13. We have extended this contig in the telomeric (t; PAX6-D11S155) and centromeric (c; D11S935-CAT) direction by hybridizing high density grid filters of the RPCI-1, -3, -4, and -5 PAC libraries with 11p13 specific markers and probes derived from a YAC contig covering 11p13 (Gawin et al 1995). 125 PAC clones for the centromeric region c and 94 for the telomeric region t were identified and used as starting material for further end probe walking.…”

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confidence: 99%

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A 7.5 Mb Sequence-Ready PAC Contig and Gene Expression Map of Human Chromosome 11p13–p14.1

Gawin

Niederführ²,

Schumacher³

et al. 1999

Genome Res.

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The region p13 of the short arm of human chromosome 11 has been studied intensely during the search for genes involved in the etiology of the Wilms' tumor, aniridia, genitourinary abnormalities, mental retardation (WAGR) syndrome, and related conditions. The gene map for this region is far from being complete, however, strengthening the need for additional gene identification efforts. We describe the extension of an existing contig map with P1-derived artificial chromosomes (PACs) to cover 7.5 Mb of 11p13-14.1. The extended sequence-ready contig was established by end probe walking and fingerprinting and consists of 201 PAC clones. Utilizing bins defined by overlapping PACs, we generated a detailed gene map containing 20 genes as well as 22 anonymous ESTs which have been identified by searching the RH databases. RH maps and our established gene map show global correlation, but the limits of resolution of the current RH panels are evident at this scale. Initial expression studies on the novel genes have been performed by Northern blot analyses. To extend these expression profiles, corresponding mouse cDNA clones were identified by database search and employed for Northern blot analyses and RNA in situ hybridizations to mouse embryo sections. Genomic sequencing of clones along a minimal tiling path through the contig is currently under way and will facilitate these expression studies by in silico gene identification approaches.

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Section: Contig Constructionmentioning

confidence: 99%

Section: Identification Of 11p13 Specific Transcripts-gene Mapmentioning

confidence: 99%

Section: Identification Of 11p13 Specific Transcripts-gene Mapmentioning

confidence: 99%

Section: Expression Analyses With Mouse Cdna Clonesmentioning

confidence: 99%

mentioning

confidence: 99%

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A 7.5 Mb Sequence-Ready PAC Contig and Gene Expression Map of Human Chromosome 11p13–p14.1

Gawin

Niederführ²,

Schumacher³

et al. 1999

Genome Res.

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A Sequence-Ready 3-Mb PAC Contig Covering 16 Breakpoints of the Wilms Tumor/Anirida Region of Human Chromosome 11p13

et al. 1998

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ESE-3, a Novel Member of an Epithelium-specific Ets Transcription Factor Subfamily, Demonstrates Different Target Gene Specificity from ESE-1

Kas

Finger

Grall

et al. 2000

Journal of Biological Chemistry

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Most cancers originate as a result of aberrant gene expression in mainly glandular epithelial tissues leading to defects in epithelial cell differentiation. The latter is governed by distinct sets of transcriptional regulators. Here we report the characterization of epitheliumspecific Ets factor, family member 3 (ESE-3), a novel member of the ESE subfamily of Ets transcription factors. ESE-3 shows highest homology to two other epithelium restricted Ets factors, ESE-1 and ESE-2. ESE-3, like ESE-1 and ESE-2, is exclusively expressed in a subset of epithelial cells with highest expression in glandular epithelium such as prostate, pancreas, salivary gland, and trachea. A potential role in branching morphogenesis is suggested, since ESE-3 transactivates the c-MET promoter via three high affinity binding sites. Additionally, ESE-3 binding to DNA sequences in the promoters of several glandular epithelium-specific genes suggests a role for ESE-3 in later stages of glandular epithelium differentiation. Although ESE-3 and ESE-1 bind with similar affinity to various Ets binding sites, ESE-3 and ESE-1 differ significantly in their ability to transactivate the promoters containing these sites. Our results support the notion that ESE-1, ESE-2, and ESE-3 represent a unique epithelium-specific subfamily of Ets factors that have critical but distinct functions in epithelial cell differentiation and proliferation.

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An Integrated YAC Clone Contig for the WAGR Region on Human Chromosome 11p13–p14.1

Cited by 5 publications

References 0 publications

A 7.5 Mb Sequence-Ready PAC Contig and Gene Expression Map of Human Chromosome 11p13–p14.1

A 7.5 Mb Sequence-Ready PAC Contig and Gene Expression Map of Human Chromosome 11p13–p14.1

A Sequence-Ready 3-Mb PAC Contig Covering 16 Breakpoints of the Wilms Tumor/Anirida Region of Human Chromosome 11p13

ESE-3, a Novel Member of an Epithelium-specific Ets Transcription Factor Subfamily, Demonstrates Different Target Gene Specificity from ESE-1

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