An integrated wearable sticker based on extended-gate AlGaN/GaN high electron mobility transistors for real-time cortisol detection in human sweat

Xu, Boxuan; Chang, Hui; Yang, Guo; Xu, Zhan; Li, Jun; Gu, Zhiqi; Li, Jiadong

doi:10.1039/d3an02115g

Cited by 3 publications

(1 citation statement)

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“…According to our previous work, when the HEMT sensors modified with electronic tags were soaked in PBS and stored at 4 °C, the performance of the HEMT sensors was degraded within a few days. 41 Therefore, the attenuation of sensitivity over time for HEMT sensors modified with different electronic tags was first studied. The initial sensitivity was defined as 1 (the sensitivity of the HEMT sensors was defined in formula (2)), and the attenuation was expressed as the attenuated sensitivity as a percentage of the initial sensitivity.…”

Section: Resultsmentioning

confidence: 99%

An integrated electronic tag-based vertical flow assay (e-VFA) with micro-sieve and AlGaN/GaN HEMT sensors for multi-target detection in actual saliva

Gu,

Chang,

Yang

et al. 2024

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Section: Resultsmentioning

confidence: 99%

An integrated electronic tag-based vertical flow assay (e-VFA) with micro-sieve and AlGaN/GaN HEMT sensors for multi-target detection in actual saliva

Gu,

Chang,

Yang

et al. 2024

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Recent advances in nonenzymatic electrochemical biosensors for sports biomarkers: focusing on antibodies, aptamers and molecularly imprinted polymers

He,

Chen,

Chu

et al. 2024

Anal. Methods

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A Gold Nanoparticle-Based Cortisol Aptasensor for Non-Invasive Detection of Fish Stress

Tanaka,

Salleh,

Tan

et al. 2024

Biomolecules

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Cortisol is a key stress biomarker in humans and animals, including fishes. In aquafarming, stress monitoring using cortisol quantification can help to optimize aquaculture practices for welfare and productivity enhancement. However, most current methods for cortisol detection rely on invasive tissue sampling. In this work, we developed a gold nanoparticle (AuNP)-based cortisol sensor to address the demand of detecting picomolar ranges of cortisol from complex fish tank water matrices as a non-invasive alternative for more effective stress monitoring. We first identified a DNA aptamer with effective binding to cortisol and then conjugated the thiol-labelled aptamer to AuNPs together with a blocker molecule (CALNN) to form an Au-Apt-CALNN conjugate that is stable in fish tank water. The cortisol detection principle is based on magnesium chloride (MgCl2)-induced particle aggregation, where the cortisol-bound aptamer on the AuNPs folds into a tertiary structure and provides greater protection for Au-Apt-CALNN against MgCl2-induced aggregation due to steric stabilization. At an optimum MgCl2 concentration, the differential stability of particles with and without cortisol binding offers a limit of detection (LOD) of 100 pM for cortisol within a 35 min reaction. The aptasensor has been validated on recirculating aquaculture system (RAS) fish tank water samples by the HPLC method and was able to detect changes in water cortisol induced by two different stress paradigms. This on-site deployable and non-invasive sensor offers opportunities for more efficient and real-time fish stress monitoring for the optimization of aquaculture practices.

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An integrated wearable sticker based on extended-gate AlGaN/GaN high electron mobility transistors for real-time cortisol detection in human sweat

Abstract: Cortisol hormone imbalances can be detected through non-invasive sweat monitoring using field-effect transistor (FET) biosensors, which provide rapid and sensitive detection. However, challenges like skin compatibility and integration with sweat...

Cited by 3 publications

References 57 publications

An integrated electronic tag-based vertical flow assay (e-VFA) with micro-sieve and AlGaN/GaN HEMT sensors for multi-target detection in actual saliva

An integrated electronic tag-based vertical flow assay (e-VFA) with micro-sieve and AlGaN/GaN HEMT sensors for multi-target detection in actual saliva

Recent advances in nonenzymatic electrochemical biosensors for sports biomarkers: focusing on antibodies, aptamers and molecularly imprinted polymers

A Gold Nanoparticle-Based Cortisol Aptasensor for Non-Invasive Detection of Fish Stress

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