An Integrated Versatile Lab-On-A-Chip Platform for the Isolation and Nucleic Acid-Based Detection of Pathogens

Sandetskaya, Natalia; Moos, Doreen; Potter, H.; Seifert, Stefan; Jenerowicz, Marcin; Becker, Holger; Zilch, Christian; Kuhlmeier, Dirk

doi:10.4155/fsoa-2016-0088

Cited by 13 publications

(11 citation statements)

References 23 publications

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“…Under the premise of ensuring phase guide filtration, a relatively large space will facilitate the movement of the magnetic particles. If there is no oil or air gaps, the integrated operation might be difficult to propel [83]. The integrated chip combines nucleic acid extraction, digital recombinase polymerase amplification (dRPA) and fluorescence detection.…”

Section: The Integrated Magnetic Nucleic Acid Detection Assaysmentioning

confidence: 99%

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Chen

Liu

Shi

et al. 2020

TrAC Trends in Analytical Chemistry

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Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre -including this research content -immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Section: The Integrated Magnetic Nucleic Acid Detection Assaysmentioning

confidence: 99%

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Chen

Liu

Shi

et al. 2020

TrAC Trends in Analytical Chemistry

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“…Alternatively, after lysis, specific nucleic acids can be captured by hybridization [ 24 , 25 ], followed by release and detection or optionally amplification/detection [ 26 ]. In another option, bacteria or viruses can be immunocaptured or fractionated by physical means, followed by lysis, nucleic acid isolation, and amplification/detection [ 27 , 28 ]. The extraction and NA isolation step removes substances that inhibit amplification [ 29 ].…”

Section: Background: Medical Diagnostics and Poc Testingmentioning

confidence: 99%

Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

et al. 2018

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Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, with emphasis on minimally instrumented, point-of-care (POC) tests for resource-limited settings. Microfluidic cartridges (‘chips’) that combine solid-phase nucleic acid extraction; isothermal enzymatic nucleic acid amplification; pre-stored, paraffin-encapsulated lyophilized reagents; and real-time or endpoint optical detection are described. These chips can be used with a companion module for separating plasma from blood through a combined sedimentation-filtration effect. Three reporter types: Fluorescence, colorimetric dyes, and bioluminescence; and a new paradigm for end-point detection based on a diffusion-reaction column are compared. Multiplexing (parallel amplification and detection of multiple targets) is demonstrated. Low-cost detection and added functionality (data analysis, control, communication) can be realized using a cellphone platform with the chip. Some related and similar-purposed approaches by others are surveyed.

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“…This strategy has been used for the separation/concentration of viruses, bacteria, parasites, protein biomarkers, nucleic acids, antibiotics, and toxins, and for bioremediation (pesticides removal) purposes. − Considering the availability and stability of ligands and the simplicity of the protocols, functionalized MPs will most likely have an increased role in sample preparation procedures.…”

Section: Sample Preparation Considerationsmentioning

confidence: 99%

Lab-on-Chip Devices: Gaining Ground Losing Size

Romao¹,

Martins²,

Germano³

et al. 2017

ACS Nano

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Portable analytical devices are notably gaining relevance in the panorama of urgent testing. Such devices have the potential to play an important role as easy-to-handle tools in critical situations. Epidemic infectious disease agents (e.g., Ebola virus, Coronavirus, Zika virus) could be controlled more easily by testing travelers on-site at the country borders to prevent outbreaks from spreading. The increasing incidence of hospital-acquired infections caused by antibiotic resistant pathogens could be minimized by point-of-care microbial analysis as well as rapid screening tests of bacteria resistance. The threat of bioterrorism using novel unknown bioweapons has never been so high, thus, in-the-field early identification of the biological agent is crucial for triggering a coordinated response. Food allergies are a growing public health concern-allergic reactions can result in anaphylactic shock, which can prove fatal in minutes-thus, the ability to test foods for common allergens, rapidly and locally, before ingestion, would improve food safety for those with allergies. Lab-on-chip devices are becoming widely available for diverse applications and are becoming increasingly affordable. However, to shrink in price and size simultaneously, some trade-offs must be made. In this Perspective, we present considerations about product specifications, design concepts, and application scenarios.

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An Integrated Versatile Lab-On-A-Chip Platform for the Isolation and Nucleic Acid-Based Detection of Pathogens

Cited by 13 publications

References 23 publications

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

Lab-on-Chip Devices: Gaining Ground Losing Size

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