An integrated system using phenylboronic acid functionalized magnetic beads and colorimetric detection for Staphylococcus aureus

Wang, Zhengzheng; Liu, Ju; Chen, Guanhua; Feng, Xiaoyan; Deng, Mei; Mu, Dan; Xu, Qiang; Xu, Hengyi

doi:10.1016/j.foodcont.2021.108633

Cited by 35 publications

(9 citation statements)

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“…As shown in Fig. 7 (a), immune magnetic separation is a simple pretreatment method based on the interaction of antigen and antibody, which is characterized by excellent anti-interference ability, rapid speed, good stability, and can rapidly enrich the target from the complicated mechanism [ 161 ]. Combination with quantum dots enables virus detection and quantification.…”

Section: Quantum Dotsmentioning

confidence: 99%

Fluorescent detection of emerging virus based on nanoparticles: From synthesis to application

Xiao

2023

TrAC Trends in Analytical Chemistry

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Section: Quantum Dotsmentioning

confidence: 99%

Fluorescent detection of emerging virus based on nanoparticles: From synthesis to application

Xiao

2023

TrAC Trends in Analytical Chemistry

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“…Therefore, in this section, we will focus on the biorecognition reagents modified on MNPs for the recognition and capture of foodborne pathogens in food (Figure 1), including antibodies (Z. Wang, Cai, et al., 2020), aptamers (Tian et al., 2021), bacteriophages (Y. Zhang et al., 2017), antibiotics (X. Yang et al., 2017), lectins (J.‐C. Yu, Hu, et al., 2018), polymers (H. Zheng et al., 2021), and others biorecognition molecules (Dechtrirat et al., 2014; S. Kim et al., 2021; Z. Wang, Liu, et al., 2022). Accordingly, Table 2 summarizes the functionalized MNPs for the recognition and separation of foodborne pathogens.…”

Section: Biorecognition Reagents Modified On Mnpsmentioning

confidence: 99%

“…Moreover, the unique advantages of hydrophilicity, biocompatibility, flexibility, water‐solubility, non‐toxicity, and non‐antigenicity allow PEG a suitable spacer for various bioconjugations (Figure 3b) (Kharazmi et al., 2020; Meng, Li, et al., 2017). In our recent studies, PEG‐mediated 3‐aminophenylboronic acid (APBA) functionalized MNPs (APBA‐PEG‐MNPs) were used to separate S. aureus in food samples, and the amount of APBA‐PEG‐MNPs was reduced by 29% compared with the direct rigid coupling method (APBA‐MNPs) while obtaining the same capture efficiency (Z. Wang, Fang, et al., 2022; Z. Wang, Liu, et al., 2022). In addition, some group‐rich proteins including bovine serum albumin (BSA) (Y. Wang, Wang, et al., 2022) and ovalbumin (G. Chen, Yang, et al., 2021), and branched polymers including dendrimers (Y. Chen et al., 2020), poly‐l‐lysine (X. Yang et al., 2017), polyethyleneimine (J. Li, Li, et al., 2019), and other polymers (Ozalp et al., 2015) are also used to mediate conjugation to increase the load of biorecognition reagents on the surface of MNPs (Figure 3c).…”

Section: Factors Affecting the Separation Of Pathogensmentioning

confidence: 99%

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Xiao

2022

Comp Rev Food Sci Food Safe

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Foodborne pathogens contamination is one of the main sources of food safety problems. Although the existing detection methods have been developed for a long time, the complexity of food samples is still the main factor affecting the detection time and sensitivity, and the rapid separation and enrichment of pathogens is still an objective to be studied. Magnetic separation strategy based on magnetic nanoparticles (MNPs) is considered to be an effective tool for rapid separation and enrichment of foodborne pathogens in food. Therefore, this study comprehensively reviews the development of MNPs in the separation of foodborne pathogens over the past decade. First, various biorecognition reagents for identification of foodborne pathogens and their modifications on the surface of MNPs are introduced. Then, the factors affecting the separation of foodborne pathogens, including the size of MNPs, modification methods, separation strategies and separation forms are discussed. Finally, the application of MNPs in integrated detection methods is reviewed. Moreover, current challenges and prospects of MNPs for the analysis of foodborne pathogens are discussed. Further research should focus on the design of multifunctional MNPs, the processing of large‐scale samples, the simultaneous analysis of multiple targets, and the development of all‐in‐one small analytical device with separation and detection.

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“…Insufficiency in boronate groups was considered one of the main reasons [ 21 ]. This may result in not only poor enrichment of target bacteria but also significant interference induced by the non-specific action with matrix components [ 22 , 23 ]. In order to improve the efficiency and simplicity of fluorescence detection in food, we considered the preparation of a thin-film boronate affinity material with high enrichment capacity.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation

Sui

Lin

et al. 2023

Foods

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There are increasing demands for fast and simple detection of pathogens in foodstuffs. Fluorescence analysis has demonstrated significant advantages for easy operation and high sensitivity, although it is usually hindered by a complex matrix, low bacterial abundance, and long-term bacterial enrichment. Effective enrichment procedures are required to meet the requirements for food detection. Here, boronate-functionalized cellulose filter paper and specific fluorescent probes were combined. An integrated approach for the enrichment of detection of Staphylococcus aureus was proposed. The modification of polyethyleneimine demonstrated a significant effect in enhancing the bacterial enrichment, and the boronate affinity efficiency of the paper was increased by about 51~132%. With optimized conditions, the adsorption efficiency for S. aureus was evaluated as 1.87 × 108 CFU/cm2, the linear range of the fluorescent analysis was 104 CFU/mL~108 CFU/mL (R2 = 0.9835), and the lowest limit of detection (LOD) was calculated as 2.24 × 102 CFU/mL. Such efficiency was validated with milk and yogurt samples. These results indicated that the material had a high enrichment capacity, simple operation, and high substrate tolerance, which had the promising potential to be the established method for the fast detection of food pathogens.

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An integrated system using phenylboronic acid functionalized magnetic beads and colorimetric detection for Staphylococcus aureus

Cited by 35 publications

References 50 publications

Fluorescent detection of emerging virus based on nanoparticles: From synthesis to application

Fluorescent detection of emerging virus based on nanoparticles: From synthesis to application

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Rapid and Sensitive Fluorescence Detection of Staphylococcus aureus Based on Polyethyleneimine-Enhanced Boronate Affinity Isolation

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