An integrated system of ABO typing and multiplex STR testing for forensic DNA analysis

Jiang, Xiaobei; Jia, Fei; Shen, Haihong; Zhao, Jinling; Chen, Chuguang; Bai, Li-Ping; Liu, Feng; Hou, Guangwei; Guo, Faye

doi:10.1016/j.fsigen.2012.03.001

Cited by 16 publications

(12 citation statements)

References 17 publications

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“…To avoid the false positive rates associated with the ABO system, blood phenotypes were also performed from endpoint multiplex reactions as in Jiang et al [45]. All amplicons were obtained with a thermal cycler Kyratec Supercycler SC200 thermal cycler (Queensland, Australia).…”

Section: Determination Of Genotypesmentioning

confidence: 99%

ALOX5,LPA,MMP9andTPOgene polymorphisms increase atherothrombosis susceptibility in middle-aged Mexicans

et al. 2020

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Atherothrombosis is the cornerstone of cardiovascular diseases and the primary cause of death worldwide. Genetic contribution to disturbances in lipid metabolism, coagulation, inflammation and oxidative stress increase the susceptibility to its development and progression. Given its multifactorial nature, the multiloci studies have been proposed as potential predictors of susceptibility. A cross-sectional study was conducted to explore the contribution of nine genes involved in oxidative stress, inflammatory and thrombotic processes in 204 subjects with atherothrombosis matched by age and gender with a healthy group ( n = 204). To evaluate the possibility of spurious associations owing to the Mexican population genetic heterogeneity as well as its ancestral origins, 300 unrelated mestizo individuals and 329 Native Americans were also included. ALOX5 , LPA , MMP9 and TPO gene polymorphisms, as well as their multiallelic combinations, were twice to four times more frequent in those individuals with clinical manifestations of atherothrombosis than in the healthy group. Once adjusting for population stratification was done, these differences remained. Our results add further evidence on the contribution of ALOX5 , LPA , MMP9 and TPO polymorphisms to atherothrombosis development in the middle-aged group, emphasizing the multiethnic studies in search of gene risk polymorphisms.

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Section: Determination Of Genotypesmentioning

confidence: 99%

ALOX5,LPA,MMP9andTPOgene polymorphisms increase atherothrombosis susceptibility in middle-aged Mexicans

et al. 2020

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“…All six ABO genotypes (O/O, B/B, A/A, A/O, A/B and B/O) could be determined by analyzing various combinations16. Mutations of FUT2 were screened by sequencing (See Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…For sperm cells samples, ABO and STR typing were first done according to the method described previously16. DNA was directly extracted from each semen sample using DNA IQ TM (Promega, USA) and eluted in 50 μL Elution buffer following manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

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Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification

Xie

Chen

et al. 2016

Sci Rep

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No effective method has been developed to distinguish sperm cells originating from different men in multi-suspect sexual assault cases. Here we combined MACS and FACS to isolate single donor sperm cells from forensic mixture samples including female vaginal epithelial cells and sperm cells from multiple contributors. Sperms from vaginal swab were isolated by MACS using FITC-conjugated A kinase anchor protein 3 (AKAP3) antibody; target individual sperm cells involving two or three donors were separated by FACS using FITC-labeled blood group A/B antigen antibody. This procedure was further tested in two mock multi-suspect sexual assault samples and one practical casework sample. Our results showed that complete single donor STR profiles could be successfully obtained from sperm/epithelial cell and sperm mixtures from two contributors. For unbalanced sperm/epithelial cells and sperm cells mixtures, sensitivity results revealed that target cells could be detected at as low as 1:32 and 1:8 mixed ratios, respectively. Although highly relies on cell number and blood types or secretor status of the individuals, this procedure would still be useful tools for forensic DNA analysis of multi-suspect sexual assault cases by the combined use of FACS and MACS based on sperm-specific AKAP3 antigen and human blood type antigen.

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“…За последние годы появилось большое количество сообщений о различных молекулярно-генетических способах установления групповой принадлежности [1][2][3][4][5][6][7][8].…”

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The identification of the AB0 blood type system by means of the single nucleotide polymorphisms analysis

Mi¹,

Nv²,

Vy³

et al. 2016

Sud.-med. ekspert.

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An integrated system of ABO typing and multiplex STR testing for forensic DNA analysis

Cited by 16 publications

References 17 publications

ALOX5,LPA,MMP9andTPOgene polymorphisms increase atherothrombosis susceptibility in middle-aged Mexicans

ALOX5,LPA,MMP9andTPOgene polymorphisms increase atherothrombosis susceptibility in middle-aged Mexicans

Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification

The identification of the AB0 blood type system by means of the single nucleotide polymorphisms analysis

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