An integrated strategy for polyploidization of Cerastigma willmottianum Stapf based on tissue culture and chemical mutagenesis and the carbon dioxide fixation ability of tetraploids

Shi, Lisha; Gao, Suping; Lei, Ting; Duan, Yifan; Yang, Lijuan; Li, Jiani; Zhao, Zian; Qin, Ling

doi:10.1007/s11240-022-02277-6

Cited by 2 publications

(3 citation statements)

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“…The failure of cytokinesis by the end of the cell cycle will cause two sets of chromosomes to remain in a single nucleus when the polyploid cell is formed [20,[22][23][24][25]. Successful methods of chemical mutagenesis for inducing polyploidy include the impregnation method [26], mixed culture method [27], and smearing method [28], among others. Typically, young and well-differentiated parts such as clustered buds and callus tissues are used as induction materials [22].…”

Section: Mutagenic Effects Of Different Mutagenesis Methods On R Roxb...mentioning

confidence: 99%

Polyploid Induction and Identification of Rosa roxburghii f. eseiosa

Jiang

et al. 2023

Plants

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Rosa roxburghii f. eseiosa Ku is a variety of Rosa roxburghii, with two known genotypes: Wuci 1 and Wuci 2. The lack of prickle on the peel of R. roxburghii f. eseiosa makes it easy to pick and process, but its fruit size is small. Therefore, we aim to induce polyploidy in order to obtain a larger fruit variety of R. roxburghii f. eseiosa. In this study, current-year stems of Wuci 1 and Wuci 2 were used as materials for polyploid induction, which was carried out through colchicine treatment coupled with tissue culture and rapid propagation technology. Impregnation and smearing methods were effectively used to produce polyploids. Using flow cytometry and a chromosome counting method, it was found that one autotetraploid of Wuci 1 (2n = 4x = 28) was obtained by the impregnation method before primary culture, with a variation rate of 1.11%. Meanwhile, seven Wuci 2 bud mutation tetraploids (2n = 4x = 28) were produced by smearing methods during the training seedling stage. When tissue-culture seedlings were treated with 20 mg/L colchicine for 15 days, the highest polyploidy rate was up to 60%. Morphological differences between different ploidys were observed. The side leaflet shape index, guard cell length, and stomatal length of the Wuci 1 tetraploid were significantly different from those of the Wuci 1 diploid. The terminal leaflet width, terminal leaflet shape index, side leaflet length, side leaflet width, guard cell length, guard cell width, stomatal length, and stomatal width of the Wuci 2 tetraploid were significantly different from those of the Wuci 2 diploid. Additionally, the leaf color of the Wuci 1 and Wuci 2 tetraploids changed from light to dark, with an initial decrease in chlorophyll content followed by an increase. In summary, this study established an effective method for inducing polyploids in R. roxburghii f. eseiosa, which could provide a foundation for the breeding and development of new genetic resources for R. roxburghii f. eseiosa and other R. roxburghii varieties in the future.

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Section: Mutagenic Effects Of Different Mutagenesis Methods On R Roxb...mentioning

confidence: 99%

Polyploid Induction and Identification of Rosa roxburghii f. eseiosa

Jiang

et al. 2023

Plants

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“…Additionally, all species are of great value in landscapes for their features of drought resistance, salinity tolerance as well as ease of cultivation [ 5 ]. And owing to their blue and violet flowers and long flowering time in autumn or winter, some are introduced as garden ornaments outside China [ 6 ]. Cerotastigma also contain a variety of chemical active ingredients such as plumbagin, which exhibits antitumor, anticancer and antibacterial activities [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Their results support the ideas of the more recent selfing avoidance model by D. & B. Charlesworth [ 11 ], in which distyly evolves from self-incompatible ancestors other than reciprocal herkogamy. Moreover, the medicinal and horticultural properties place the genus as target studies regarding establishing rapid propagation systems for obtaining plumbagin and developing polyploids used in landscapes [ 6 ]. Lastly, as we mentioned above, Plumbaginaceae including Ceratostigma species could survive in harsh environmental conditions like high salt media or heavy-metal-rich soils, and some researchers found a strong correspondence between these capacities and the secretory structures such as salt glands secreting a range of ions, which might have arisen as a means to avoid the toxicity or regulate ion concentrations within leaves [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of plastid genomes in the genus Ceratostigma inhabiting arid habitats in China and their phylogenomic implications

et al. 2023

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Background Ceratostigma, a genus in the Plumbaginaceae, is an ecologically dominant group of shrubs, subshrub and herb mainly distributed in Qinghai-Tibet Plateau and North China. Ceratostigma has been the focal group in several studies, owing to their importance in economic and ecological value and unique breeding styles. Despite this, the genome information is limited and interspecific relationships within the genus Cerotastigma remains unexplored. Here we sequenced, assembled and characterized the 14 plastomes of five species, and conducted phylogenetic analyses of Cerotastigma using plastomes and nuclear ribosomal DNA (nrDNA) data. Results Fourteen Cerotastigma plastomes possess typical quadripartite structures with lengths from 164,076 to 168,355 bp that consist of a large single copy, a small single copy and a pair of inverted repeats, and contain 127–128 genes, including 82–83 protein coding genes, 37 transfer RNAs and eight ribosomal RNAs. All plastomes are highly conservative and similar in gene order, simple sequence repeats (SSRs), long repeat repeats and codon usage patterns, but some structural variations in the border of single copy and inverted repeats. Mutation hotspots in coding (Pi values > 0.01: matK, ycf3, rps11, rps3, rpl22 and ndhF) and non-coding regions (Pi values > 0.02: trnH-psbA, rps16-trnQ, ndhF-rpl32 and rpl32-trnL) were identified among plastid genomes that could be served as potential molecular markers for species delimitation and genetic variation studies in Cerotastigma. Gene selective pressure analysis showed that most protein-coding genes have been under purifying selection except two genes. Phylogenetic analyses based on whole plastomes and nrDNA strongly support that the five species formed a monophyletic clade. Moreover, interspecific delimitation was well resolved except C. minus, individuals of which clustered into two main clades corresponding to their geographic distributions. The topology inferred from the nrDNA dataset was not congruent with the tree derived from the analyses of the plastid dataset. Conclusion These findings represent the first important step in elucidating plastome evolution in this widespread distribution genus Cerotastigma in the Qinghai-Tibet Plateau. The detailed information could provide a valuable resource for understanding the molecular dynamics and phylogenetic relationship in the family Plumbaginaceae. Lineage genetic divergence within C. minus was perhaps promoted by geographic barriers in the Himalaya and Hengduan Mountains region, but introgression or hybridization could not be completely excluded.

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An integrated strategy for polyploidization of Cerastigma willmottianum Stapf based on tissue culture and chemical mutagenesis and the carbon dioxide fixation ability of tetraploids

Cited by 2 publications

References 45 publications

Polyploid Induction and Identification of Rosa roxburghii f. eseiosa

Polyploid Induction and Identification of Rosa roxburghii f. eseiosa

Characteristics of plastid genomes in the genus Ceratostigma inhabiting arid habitats in China and their phylogenomic implications

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