An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A (TBBPA)

Ji, Chenglong; Li, Fei; Wang, Qing; Zhao, Jianmin; Sun, Zuodeng; Wu, Huifeng

doi:10.1016/j.chemosphere.2015.08.052

Cited by 48 publications

(24 citation statements)

References 54 publications

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“…Free amino acids account for a large fraction of the metabolome of marine invertebrates (Cappello et al, 2013) and can be oxidized to supply energy in the Krebs cycle. When the oyster (Crassostrea sikamea) was exposed to metal pollution, amino acids including threonine, alanine, arginine, glutamate, beta-alanine, aspartate and glycine decreased significantly (Ji et al, 2016). Exposure to Cu 2+ could result in alterations of 25 metabolites involved in oxidative stress responses and apoptosis processes in mussel Perna canaliculus (Nguyen et al, 2018a).…”

Section: Amino Acid Metabolismmentioning

confidence: 99%

“…Gas chromatography coupled with mass spectrometry (GC/MS) is a well-established analytical method that can provide a comprehensive and systematic understanding of all metabolites in biological samples (Tsugawa et al, 2011;Nguyen & Alfaro, 2019a). GC/MS-based metabolomics has been widely applied to study the physiological responses of aquatic organisms to environmental stressors, including pathogen infection, water contaminants and aerial exposure, and many metabolites and associated pathways have been successfully identified (Guo et al, 2014;Ji et al, 2016;Chen et al, 2015;Nguyen et al, 2018aNguyen et al, , 2018c, Alfaro, Nguyen & Mellow, 2019.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Jiang

Jiao

Sun

et al. 2020

PeerJ

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Marine mollusks are commonly subjected to heat stress. To evaluate the effects of heat stress on the physiological metabolism of the ark shell Scapharca subcrenata, clams were exposed to different high temperatures (24, 28 and 32 °C) for 72 h. The oxygen consumption and ammonia excretion rates were measured at 2, 12, 24, 48 and 72 h. The results indicated that the metabolic rates of the ark shell significantly increased with increasing heat stress, accompanied by mortalities in response to prolonged exposure. A metabolomics approach based on gas chromatography coupled with mass spectrometry was further applied to assess the changes of metabolites in the mantle of the ark shell at 32 °C. Moreover, multivariate and pathway analyses were conducted for the different metabolites. The results showed that the heat stress caused changes in energy metabolism, amino acid metabolism, osmotic regulation, carbohydrate metabolism and lipid metabolism through different metabolic pathways. These results are consistent with the significant changes of oxygen consumption rate and ammonia excretion rate. The present study contributes to the understanding of the impacts of heat stress on intertidal bivalves and elucidates the relationship between individual-level responses and underlying molecular metabolic dynamics.

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Section: Amino Acid Metabolismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Jiang

Jiao

Sun

et al. 2020

PeerJ

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Section: Amino Acid Metabolismmentioning

confidence: 99%

Peer Review #1 of "Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics (v0.2)"

Nguyen¹

2020

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Marine mollusks are commonly subjected to heat stress. To evaluate the effects of heat stress on the physiological metabolism of the ark shell Scapharca subcrenata, clams were exposed to different high temperatures (24, 28, and 32 °C) for 72 h. The oxygen consumption and ammonia excretion rates were measured at 2, 12, 24, 48, and 72 h. The results indicated that the metabolic rates of the ark shell significantly increased with increasing heat stress, accompanied by mortalities in response to prolonged exposure. A metabolomics approach based on gas chromatography coupled with mass spectrometry was further applied to assess the changes of metabolites in the mantle of the ark shell at 32 °C. Moreover, multivariate and pathway analyses were conducted for the different metabolites. The results showed that the heat stress caused changes in energy metabolism, amino acid metabolism, osmotic regulation, carbohydrate metabolism, and lipid metabolism through different metabolic pathways. These results are consistent with the significant changes of oxygen consumption rate and ammonia excretion rate. The present study contributes to the understanding of the impacts of heat stress on intertidal bivalves and elucidates the relationship between individual-level responses and underlying molecular metabolic dynamics.

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“…In addition, iTRAQ or TMT based methods have been developed. For the studies on sex-based differences, also use of iTRAQ (Carlson et al 2013;Fong et al 2014;Ji et al 2014;Wang et al 2014;Al-Daghri et al 2016;Ji et al 2016;Le Manach et al 2016;Yang et al 2016) or sometimes TMT (Tandem Mass Tag) (Chen et al 2014) was encountered (Table 1). Using these techniques, peptides are labeled with a stable isotope detection tag after trypsin digestion.…”

Section: Introductionmentioning

confidence: 99%

Use of proteomics to detect sex-related differences in effects of toxicants: implications for using proteomics in toxicology

Rietjens

Vervoort

Maslowska-Gornicz

et al. 2018

Critical Reviews in Toxicology

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This review provides an overview of results obtained when using proteome analysis for detecting sexbased differences in response to toxicants. It reveals implications to be taken into account when considering the use of proteomics in toxicological studies. It appears that results may differ when studying the same chemical in the same species in different target tissues. Another result of interest is the limited dose-response behavior of differential abundance patterns observed in studies where more than one dose level is tested. It is concluded that use of proteomics to study differences in modes of action of toxic compounds is an active area of research. The examples from use of proteomics to study sexdependent differences also reveal that further studies are needed to provide reliable insight in modes of action, novel biomarkers or even novel therapies. To eventually reach this aim for this and other toxicological endpoints, it is essential to consider background variability, consequences of timing of toxicant administration, dose-response behavior, relevant species and target organ, species and organ variability and the presence of proteoforms.

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An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A (TBBPA)

Cited by 48 publications

References 54 publications

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics

Peer Review #1 of "Metabolic response of Scapharca subcrenata to heat stress using GC/MS-based metabolomics (v0.2)"

Use of proteomics to detect sex-related differences in effects of toxicants: implications for using proteomics in toxicology

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