An integrated process to produce prebiotic xylooligosaccharides by autohydrolysis, nanofiltration and endo-xylanase from alkali-extracted xylan

Lian, Zhina; Wang, Yane; Luo, Jing; Lai, Chenhuan; Yong, Qiang; Yu, Shiyuan

doi:10.1016/j.biortech.2020.123685

Cited by 72 publications

(21 citation statements)

References 34 publications

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“…The optimized combination is A 1 B 3 C 2 D 1 was established from analyzing the K values and a validation test was done in which the yield of XOS was 34.2%, higher than that the highest value listed in Table 3 . Therefore, the optimal conditions for XOS production with xylanase XynAR were 60 °C, pH 5, enzyme loading of 15 U/mL, and a hydrolysis period 2 h. The XOS yield from Jiuzao was lower than that of previous reports using other substrates [ 45 , 46 , 47 ]. Aside from the ingredient differences, perhaps production from Jiuzao was lower because some hemicellulose structures from rice hulls were decomposed under the combined action of high temperature treatment and microbial activity during the Baijiu production process [ 12 , 33 ].…”

Section: Resultsmentioning

confidence: 89%

Production of Xylooligosaccharides from Jiuzao by Autohydrolysis Coupled with Enzymatic Hydrolysis Using a Thermostable Xylanase

Qin

Tian

et al. 2022

Foods

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The production of xylooligosaccharides (XOS) from Jiuzao was studied using a two-stage process based on autohydrolysis pretreatment followed by enzymatic hydrolysis. Jiuzao was autohydrolyzed under conditions where temperature, time, particle size, and solid-liquid ratio were varied experimentally. Optimal XOS production was obtained from Jiuzao with a >20 mesh particle size treated at 181.5 °C for 20 min with a 1:13.6 solid-liquid ratio. Subsequently, optimal enzymatic hydrolysis conditions for xylanase XynAR were identified as 60 °C, pH 5, and xylanase XynAR loading of 15 U/mL. Using these conditions, a yield of 34.2% XOS was obtained from Jiuzao within 2 h. The process developed in the present study could enable effective and ecofriendly industrial production of XOS from Jiuzao.

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Section: Resultsmentioning

confidence: 89%

Production of Xylooligosaccharides from Jiuzao by Autohydrolysis Coupled with Enzymatic Hydrolysis Using a Thermostable Xylanase

Qin

Tian

et al. 2022

Foods

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“…The percentage and composition of these ramifications is strongly determined by the xylan source [10]. Xylans can be hydrolyzed by chemical (autohydrolysis) [11] or enzymatic methods [12][13][14]. The latter are more convenient since they do not generate undesired by-products [15,16].…”

Section: Introductionmentioning

confidence: 99%

Enzymatic bioconversion of beechwood xylan into the antioxidant 2′-O-α-(4-O-methyl-D-glucuronosyl)-xylobiose

Míguez

Fernandez-Polo

Santos-Moriano

et al. 2022

Biomass Conv. Bioref.

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Acidic xylooligosaccharides (XOS), also called aldouronics, are hetero-oligomers of xylose randomly branched with 4-O-methyl-D-glucuronic acid residues linked by α(1 → 2) bonds, which display bioactive properties. We have developed a simple and integrated method for the production of acidic XOS by enzymatic hydrolysis of a glucurono-xylan from beechwood. Among the enzymes screened, Depol 670L (a cellulolytic preparation from Trichoderma reesei) displayed the highest activity (70.3 U/mL, expressed in reducing xylose equivalents). High-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) analysis revealed the formation of a neutral fraction (corresponding to linear XOS, mainly xylose and xylobiose) and a group of more retained products (acidic XOS), which were separated using strong anion-exchange cartridges. The acidic fraction contained a major product, characterized by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and mono- and two-dimensional nuclear magnetic resonance spectroscopy (NMR) as 2′-O-α-(4-O-methyl-α-D-glucuronosyl)-xylobiose (X2_MeGlcA). Starting from 2 g of beechwood xylan, 1.5 g of total XOS were obtained, from which 225 mg (11% yield) corresponded to the aldouronic X2_MeGlcA. The acidic XOS exhibited higher antioxidant activity (measured by the ABTS·+ discoloration assay) than xylan, whilst neutral XOS displayed no antioxidant activity. This work demonstrates that it is possible to obtain a safe and natural antioxidant by enzymatic biotransformation of hardwood hemicellulose.

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“…However, the effects of this step on cellulases are inconclusive, and more research in this area is required. An integrated process employed by Lian et al [ 69 ], where autohydrolysis, nanofiltration and xylanase hydrolysis are combined to give a prebiotic that is processed better than traditional multi-process techniques is an attractive novel approach. An alternative is to engineer proteins by inducing deliberate mutations, seeking structure–function relationships, to give suitable results [ 70 ].…”

Section: Introductionmentioning

confidence: 99%

Experimental and computational studies of cellulases as bioethanol enzymes

et al. 2022

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Bioethanol industries and bioprocesses have many challenges that constantly impede commercialization of the end product. One of the bottlenecks in the bioethanol industry is the challenge of discovering highly efficient catalysts that can improve biomass conversion. The current promising bioethanol conversion catalysts are microorganism-based cellulolytic enzymes, but lack optimization for high bioethanol conversion, due to biological and other factors. A better understanding of molecular underpinnings of cellulolytic enzyme mechanisms and significant ways to improve them can accelerate the bioethanol commercial production process. In order to do this, experimental methods are the primary choice to evaluate and characterize cellulase’s properties, but they are time-consuming and expensive. A time-saving, complementary approach involves computational methods that evaluate the same properties and improves our atomistic-level understanding of enzymatic mechanism of action. Theoretical methods in many cases have proposed research routes for subsequent experimental testing and validation, reducing the overall research cost. Having a plethora of tools to evaluate cellulases and the yield of the enzymatic process will aid in planning more optimized experimental setups. Thus, there is a need to connect the computational evaluation methods with the experimental methods to overcome the bottlenecks in the bioethanol industry. This review discusses various experimental and computational methods and their use in evaluating the multiple properties of cellulases.

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An integrated process to produce prebiotic xylooligosaccharides by autohydrolysis, nanofiltration and endo-xylanase from alkali-extracted xylan

Cited by 72 publications

References 34 publications

Production of Xylooligosaccharides from Jiuzao by Autohydrolysis Coupled with Enzymatic Hydrolysis Using a Thermostable Xylanase

Production of Xylooligosaccharides from Jiuzao by Autohydrolysis Coupled with Enzymatic Hydrolysis Using a Thermostable Xylanase

Enzymatic bioconversion of beechwood xylan into the antioxidant 2′-O-α-(4-O-methyl-D-glucuronosyl)-xylobiose

Experimental and computational studies of cellulases as bioethanol enzymes

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