An integrated phenol ‘sensoremoval’ microfluidic nanostructured platform

Mayorga‐Martinez, Carmen C.; Hlavata, Lenka; Miserere, Sandrine; Marzo, Adaris M. López; Labuda, Ján; Pons, Josefina; Merkoçi, Arben

doi:10.1016/j.bios.2013.12.035

Cited by 13 publications

(5 citation statements)

References 22 publications

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“…The plot of potential variation vs the amount of Ag gives a straight line (R 2 = 0.97871) ( Fig. 4 ) which confirmed the reproducibility of the measurements [ 35 ]. The limit of detection (LoD) and the limit of quantification (LoQ) were calculated using the formulae LoD = (3 × s b )/ S and LoQ = (10 × s b )/ S , respectively, where s b represents the standard deviation of the blank signal and S represents the sensitivity of the calibration curve.…”

Section: Resultssupporting

confidence: 63%

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

Argoubi¹,

Saadaoui²,

Aoun³

et al. 2015

Beilstein J. Nanotechnol.

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SummaryThe demand for on-site nanodevices is constantly increasing. The technology development for the design of such devices is highly regarded. In this work, we report the design of a disposable platform that is structured with cauliflower-shaped gold nanoparticles (cfAuNPs) and we show its applications in immunosensing and enzyme-based detection. The electrochemical reduction of Au(III) allows for the electrodeposition of highly dispersed cauliflower-shaped gold nanoparticles on the surface of screen-printed carbon electrodes (SPCEs). The nanostructures were functionalized using ferrocenylmethyl lipoic acid ester which allowed for the tethering of the ferrocene group to gold, which serves as an electrochemical transducer/mediator. The bioconjugation of the surface with anti-human IgG antibody (α-hIgG) or horseradish peroxidase (HRP) enzyme yields biosensors, which have been applied for the selective electrochemical detection of human IgG (hIgG) or H2O2 as model analytes, respectively. Parameters such as the number of sweeps, amount of charge generated from the oxidation of the electrodeposited gold, time of incubation and concentration of the ferrocene derivatives have been studied using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). Selectivity and specificity tests have been also performed in the presence of potentially interfering substances to either hIgG or H2O2. Results showed that the devised immunosensor is endowed with good selectivity and specificity in the presence of several folds of competitive analytes. The enzyme-based platform showed a good catalytic activity towards H2O2 oxidation which predestined it to potential applications pertaining to enzymatic kinetics studies. The levels of hIgG in human serum and H2O2 in honey were successfully determined and served as assessment tools of the applicability of the platforms for real samples analysis.

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Section: Resultssupporting

confidence: 63%

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

Argoubi¹,

Saadaoui²,

Aoun³

et al. 2015

Beilstein J. Nanotechnol.

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show abstract

“…The obtained biosensing performance was LOD: 10 nM; LR: 0.5 to 5 µM [173]. The same microdevice (Figure 8c) was used for the detection of phenols via electrochemical impedance spectroscopy (EIS) [148]. They obtained good analytical performance in phenol detection in terms of reproducibility, selectivity, sensitivity, and limit of detection (LR: 0.01-10 µM and LOD: 4.64 nM).…”

Section: Microfluidic Detection Systems For Phenols or Phenolic Compo...mentioning

confidence: 96%

“…Due to its instrumental simplicity, availability, flexibility, rapid analysis with high accuracy, low manufacturing costs, and facile implementation, microfluidic devices coupled with electrochemical detection are more advantageous compared to traditional electrochemical detection systems [100]. The main electrochemical detection methods for microfluidics applied for the detection and monitoring of environmental contaminants are (a) (chrono)amperometry [143]; (b) voltammetry, such as square-wave anodic stripping voltammetry (SWASV) [72,73], differential pulse anodic stripping voltammetry (DPASV) [144], cyclic voltammetry (CV) [30], or linear sweep voltammetry (LSV) [145]; (c) conductometry [30,146]; (d) potentiometry [147]; and (e) electrochemical impedance spectroscopy [148].…”

Section: Microfluidic Detection Systems For Pollution Analysismentioning

confidence: 99%

Microfluidic Devices and Microfluidics-Integrated Electrochemical and Optical (Bio)Sensors for Pollution Analysis: A Review

et al. 2022

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An overview of the recent research works and trends in the design and fabrication of microfluidic devices and microfluidics-integrated biosensors for pollution analysis and monitoring of environmental contaminants is presented in this paper. In alignment with the tendency in miniaturization and integration into “lab on a chip” devices to reduce the use of reagents, energy, and implicit processing costs, the most common and newest materials used in the fabrication of microfluidic devices and microfluidics-integrated sensors and biosensors, the advantages and disadvantages of materials, fabrication methods, and the detection methods used for microfluidic environmental analysis are synthesized and evaluated.

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“…Mayorga- Martinez et al (2014) (Fig. 6.6d) have reported an integrated phenol sensoremoval microfluidic nanostructured platform.…”

Section: Enzymatic-based Loc Biosensorsmentioning

confidence: 99%

Lab-on-a-Chip Devices and Micro-Total Analysis Systems

Castillo-León¹,

Svendsen²

2015

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An integrated phenol ‘sensoremoval’ microfluidic nanostructured platform

Cited by 13 publications

References 22 publications

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

Microfluidic Devices and Microfluidics-Integrated Electrochemical and Optical (Bio)Sensors for Pollution Analysis: A Review

Lab-on-a-Chip Devices and Micro-Total Analysis Systems

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