An integrated microfluidic system for counting of CD4+/CD8+ T lymphocytes

Wang, Jung-Hao; Wang, Chih‐Hung; Lin, Chien-Chih; Lei, Huan‐Yao; Lee, Gwo‐Bin

doi:10.1007/s10404-010-0687-z

Cited by 29 publications

(28 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research in the field of microflow cytometry concentrates on four main areas (1-5): focusing of particles in microfluidic channels, miniaturized fluid handling (6)(7)(8)(9), integration of optical elements into the chip (10)(11)(12)(13)(14), and applications development, e.g., for biomedical purposes. When analyzing micro particles, focusing of the sample stream is usually applied to restrict their trajectories inside the flow channel (1,3,5,6,(8)(9)(10)(11)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26).…”

mentioning

confidence: 99%

A microflow cytometer exploited for the immunological differentiation of leukocytes

et al. 2011

View full text Add to dashboard Cite

In this article, we demonstrate the potential of a microfluidic chip for the differentiation of immunologically stained blood cells. To this end, white blood cells stained with antibodies typically applied for the determination of the immune status were measured in the micro-device. Relative concentrations of lymphocytes and subpopulations of lymphocytes are compared to those obtained with a conventional flow cytometer. The stability of the hydrodynamic focusing and the optical setup was determined by measuring the variation of the signal pulse height of fluorescence calibration beads, being about 2% for the micro-device. This value and the overall performance of the microdevice are similar to conventional flow cytometers. It follows from our results that such microfluidic structures are well suited as modules in a compact, portable read-out instrument. The production process of the microflow cytometers, which we exploited for immunological cell differentiation, is compatible with mass production technology like injection molding and, hence, low cost disposable chips could be available in the future. '

show abstract

mentioning

confidence: 99%

A microflow cytometer exploited for the immunological differentiation of leukocytes

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Wang et al have developed a microfluidic chip that first labels CD4+ and CD8+ T lymphocytes (from buffy coat samples) on-chip using pneumatic vortexing before hydrodynamically focusing and laser-induced fluorescence (LIF) counting [111]. They were able to produce CD4/CD8 T cell ratios similar to that found using standard flow cytometry.…”

Section: B Global Health Solutions 1) Optical Methodsmentioning

confidence: 99%

Micro- and Nanotechnology for HIV/AIDS Diagnostics in Resource-Limited Settings

Damhorst

Watkins

Bashir

2013

IEEE Trans. Biomed. Eng.

View full text Add to dashboard Cite

“…Microflow cytometers provide a high-throughput means of sorting and counting cells/particles and are used in a wide variety of biomedical and industrial applications, including clinical hematology diagnosis, bacteria analysis, and gene diagnosis. [15][16][17][18][19][20] In a typical microflow cytometer, the cells/particles are injected into an electrolyte solution and are then focused in the horizontal plane by two sheath flows such that they pass through the detection region of the device in a single line fashion. 21,22 Fu et al 23 developed an electrokinetically driven microflow cytometer in which the sample was focused in the horizontal a) Author to whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

A hydrodynamic focusing microchannel based on micro-weir shear lift force

et al. 2012

View full text Add to dashboard Cite

A novel microflow cytometer is proposed in which the particles are focused in the horizontal and vertical directions by means of the Saffman shear lift force generated within a micro-weir microchannel. The proposed device is fabricated on stress-relieved glass substrates and is characterized both numerically and experimentally using fluorescent particles with diameters of 5 lm and 10 lm, respectively. The numerical results show that the micro-weir structures confine the particle stream to the center of the microchannel without the need for a shear flow. Moreover, the experimental results show that the particles emerging from the micro-weir microchannel pass through the detection region in a one-by-one fashion. The focusing effect of the micro-weir microchannel is quantified by computing the normalized variance of the optical detection signal intensity. It is shown that the focusing performance of the micro-weir structure is equal to 99.76% and 99.57% for the 5-lm and 10-lm beads, respectively. Overall, the results presented in this study confirm that the proposed microcytometer enables the reliable sorting and counting of particles with different diameters. V C 2012 American Institute of Physics. [http://dx

show abstract

An integrated microfluidic system for counting of CD4+/CD8+ T lymphocytes

Abstract: This study reports on an integrated microfluidic system capable of counting CD4 ? /CD8 ?

Cited by 29 publications

References 40 publications

A microflow cytometer exploited for the immunological differentiation of leukocytes

A microflow cytometer exploited for the immunological differentiation of leukocytes

Micro- and Nanotechnology for HIV/AIDS Diagnostics in Resource-Limited Settings

A hydrodynamic focusing microchannel based on micro-weir shear lift force

Contact Info

Product

Resources

About