An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood

Lee, Do‐Hyun; Li, Xuan; Jiang, Alan; Lee, Abraham P.

doi:10.1063/1.5049149

Cited by 18 publications

(12 citation statements)

References 35 publications

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“…Their devices used electrodes to induce nDEP to direct cells to the channel center. In our case, we hoped to direct cells to the channel outer edges and therefore employed a sheathless hydrophoretic cell aligner (Song and Choi, 2014; Song et al, 2015a; Lee et al, 2018b). We improved upon previous device designs by incorporating a gradual increase in the angle of the PDMS microstructures on the channel ceiling.…”

Section: Discussionmentioning

confidence: 99%

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High-throughput continuous dielectrophoretic separation of neural stem cells

et al. 2019

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We created an integrated microfluidic cell separation system that incorporates hydrophoresis and dielectrophoresis modules to facilitate high-throughput continuous cell separation. The hydrophoresis module consists of a serpentine channel with ridges and trenches to generate a diverging fluid flow that focuses cells into two streams along the channel edges. The dielectrophoresis module is composed of a chevron-shaped electrode array. Separation in the dielectrophoresis module is driven by inherent cell electrophysiological properties and does not require cell-type-specific labels. The chevron shape of the electrode array couples with fluid flow in the channel to enable continuous sorting of cells to increase throughput. We tested the new system with mouse neural stem cells since their electrophysiological properties reflect their differentiation capacity (e.g., whether they will differentiate into astrocytes or neurons). The goal of our experiments was to enrich astrocyte-biased cells. Sorting parameters were optimized for each batch of neural stem cells to ensure effective and consistent separations. The continuous sorting design of the device significantly improved sorting throughput and reproducibility. Sorting yielded two cell fractions, and we found that astrocyte-biased cells were enriched in one fraction and depleted from the other. This is an advantage of the new continuous sorting device over traditional dielectrophoresis-based sorting platforms that target a subset of cells for enrichment but do not provide a corresponding depleted population. The new microfluidic dielectrophoresis cell separation system improves label-free cell sorting by increasing throughput and delivering enriched and depleted cell subpopulations in a single sort.

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Section: Discussionmentioning

confidence: 99%

“…The HOAPES device is comprised of three main sections: a filter, a sheathless hydrophoretic cell aligner, and a DEP module with oblique parallel electrodes adapted from an earlier design (Lee et al, 2018b). For full dimensions of the HOAPES device, see Fig.…”

Section: Methodsmentioning

confidence: 99%

High-throughput continuous dielectrophoretic separation of neural stem cells

et al. 2019

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“…The system has a high single-cell trapping efficiency and can be used for cell analysis. D.-H. Lee et al applied a microstructure array for size-selective single-cell trapping, which was established on a microfluidic platform as described in Figure 3 b [ 50 ]. The developed platform analyzed fully integrated blood samples that enable size-selective cell separation and high-efficiency capture of single cells from diluted blood samples.…”

Section: Various Technologies Of Single-cell Trapping On Microfluidic Impedance Biosensorsmentioning

confidence: 99%

A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis

et al. 2021

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Electrical impedance biosensors combined with microfluidic devices can be used to analyze fundamental biological processes for high-throughput analysis at the single-cell scale. These specialized analytical tools can determine the effectiveness and toxicity of drugs with high sensitivity and demonstrate biological functions on a single-cell scale. Because the various parameters of the cells can be measured depending on methods of single-cell trapping, technological development ultimately determine the efficiency and performance of the sensors. Identifying the latest trends in single-cell trapping technologies afford opportunities such as new structural design and combination with other technologies. This will lead to more advanced applications towards improving measurement sensitivity to the desired target. In this review, we examined the basic principles of impedance sensors and their applications in various biological fields. In the next step, we introduced the latest trend of microfluidic chip technology for trapping single cells and summarized the important findings on the characteristics of single cells in impedance biosensor systems that successfully trapped single cells. This is expected to be used as a leading technology in cell biology, pathology, and pharmacological fields, promoting the further understanding of complex functions and mechanisms within individual cells with numerous data sampling and accurate analysis capabilities.

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“…In a second paper, another device presented by Lee et al expanded on this concept. 177 Here, human U937 monocytes (a type of WBC) were separated from diluted blood in which they were spiked at a 1:1000 dilution. Similar to the other device from the Lee group, RBCs and small WBCs were first filtered out in the microfiltration structure with target monocytes ending up in a second, downstream trapping structure.…”

Section: Microfluidicsmentioning

confidence: 99%