An integrated microfluidic device utilizing vancomycin conjugated magnetic beads and nanogold-labeled specific nucleotide probes for rapid pathogen diagnosis

Wang, Chih‐Hung; Chang, Chia-Jung; Wu, Jhao Ying; Lee, Gwo‐Bin

doi:10.1016/j.nano.2013.10.013

Cited by 40 publications

(17 citation statements)

References 29 publications

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“…For example, the capture efficiency of E. coli using DEAE magnetic beads was reported to be near 100 % (Yang et al 2011 ), but the cells were suspended in H 2 O and incubated for 20 min. Other approaches using beads functionalized with antibodies (Zhu et al 2011 ; Wen et al 2013 ; Cho et al 2014 ; Suh et al 2014 ), aptamers (Suh et al 2014 ) or vancomycin (Wang et al 2014 ), generally require longer incubation times to achieve maximum capture efficiencies ranging from 2–98, 13 and 23 %, respectively. Additionally, these tests were only performed with bacteria suspended in either buffer or water.…”

Section: Resultsmentioning

confidence: 99%

Simple and rapid sample preparation system for the molecular detection of antibiotic resistant pathogens in human urine

Valiadi

Kalsi

Jones

et al. 2016

Biomed Microdevices

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Antibiotic resistance in urinary tract infections (UTIs) can cause significant complications without quick detection and appropriate treatment. We describe a new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Klebsiella pneumoniae NCTC13443 (blaCTX-M-15 positive) spiked into filtered human urine was used as a model system. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 min using a custom, valve-less microfluidic chip. Eight samples were processed in parallel, and DNA was released using heat lysis from an integrated resistive heater. The crude cell lysate was used for real time Recombinase Polymerase Amplification (RPA) of the blaCTX-M-15 gene. The end to end processing time was approximately 15 min with a limit of detection of 1000 bacteria in 1 mL urine.Electronic supplementary materialThe online version of this article (doi:10.1007/s10544-016-0031-9) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Simple and rapid sample preparation system for the molecular detection of antibiotic resistant pathogens in human urine

Valiadi

Kalsi

Jones

et al. 2016

Biomed Microdevices

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“…The binding capacity of the screened oligopeptides with target cancer cells was evaluated. The protocol for the conjugation of the oligopeptides to the magnetic beads can be found in a previous work 42 . Dilutions of 100, 10, and 1 nM of the oligopeptide I were conjugated to Dynabeads ® MyOne TM carboxylic acid beads (Cat No.…”

Section: Methodsmentioning

confidence: 99%

Cancer Cell-Specific Oligopeptides Selected by an Integrated Microfluidic System from a Phage Display Library for Ovarian Cancer Diagnosis

Wang¹,

Weng²,

Jui³

et al. 2015

Theranostics

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Ovarian cancer is one of the leading causes of female mortality worldwide. Unfortunately, there are currently few high-specificity candidate oligopeptide targeting agents that can be used for early diagnosis of this cancer. It has been suggested that cancer-specific oligopeptides could be screened from a phage display library. However, conventional methods are tedious, labor-intensive, and time consuming. Therefore, a novel, integrated microfluidic system was developed to automate the entire screening process for ovarian cancer cell-specific oligopeptides. An oligopeptide screened with microfluidic chip-based technique was demonstrated to have high affinity to ovarian cancer cells and demonstrated relatively low binding to other cancer cells, indicating a high specificity. Furthermore, the developed method consumed relatively low volumes of samples and reagents; only 70 μL of reactant was used within the whole experimental process. Each panning process was also significantly shortened to only 7.5 hours. Therefore, the screened oligopeptide could be used to isolate ovarian cancer cells in a rapid manner, thus greatly expediting the diagnosis and its application as oligopeptide targeting agent for theranostics of this cancer.

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“…To date, various liquids have been identified by different methods such as liquid chromatography [ [24] , [25] ] and microfluidics [ [26] , [27] , [28] , [29] ]. First, the molecules are separated and then detected by various methods such as ultraviolet, photoluminescence, fluorescence, electrochemical, refractive index and electrical conductivity.…”

Section: Ptsi/porous Si Schottky Junctionmentioning

confidence: 99%

Ultrasensitive bio-detection using single-electron effect

Ashoori

Naderpour

Ghezelayagh

et al. 2021

Talanta

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Nanosized particles can detect minute changes in surrounding electric fields via a phenomenon known as single-electron effect. Sensitivity can reach as much as counting single electrons. This phenomenon promises the possibilty of detection of atoms, molecules, cells or pathogens passing over or suspended around the nanosized particles whose IV characteristics is being monitored. Unfortunately, practical realization of such devices has been challenging because of their small size and low operating temperature, well below freezing temperature of regular liquids. Here we introduce PtSi/porous Si Schottky junction as a room temperature large size single-electron device capable of detecting very minute changes in the electric fields and hence identifying molecules, cells, and bacteria suspended or dissolved in liquid solutions. Encouraging results for detection of healthy and cancerous human cells as well as liquids and microorganisms are provided.

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An integrated microfluidic device utilizing vancomycin conjugated magnetic beads and nanogold-labeled specific nucleotide probes for rapid pathogen diagnosis

Cited by 40 publications

References 29 publications

Simple and rapid sample preparation system for the molecular detection of antibiotic resistant pathogens in human urine

Simple and rapid sample preparation system for the molecular detection of antibiotic resistant pathogens in human urine

Cancer Cell-Specific Oligopeptides Selected by an Integrated Microfluidic System from a Phage Display Library for Ovarian Cancer Diagnosis

Ultrasensitive bio-detection using single-electron effect

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