An integrated microfluidic device for studying controllable gas embolism induced cellular responses

Ma, Pengtao; Wang, Shanshan; Guan, Ruixue; Hu, Liang; Wang, Xixian; Ge, Anle; Zhu, Jinchi; Du, Wei; Liu, Bi‐Feng

doi:10.1016/j.talanta.2019.120484

Cited by 5 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, based on retrospective studies, it was established that gas embolism is one of the major critical issues in intensive care units. 22,23 While the incidence and the outcomes of gas embolism were comprehensively reported, 24 only a few recent studies [25][26][27][28][29] explored the dynamics of the bubble behaviour physically simulated by in vitro experiments. The studies explored the behaviour of gas bubbles in PDMS-based microchannels under different flow properties like gas bubble behaviour in low capillary number microchannels; 26 roles of the cell-free layer and cell local concentration in the persistence of the bubble; 25 impacts of the T-junction on bubble break-up; 27 flow of blood cells around the bubble in PDMS-fabricated blood vessels; 28 and use of microfluidic devices for studying cellular responses to gas embolism.…”

Section: Introductionmentioning

confidence: 99%

“…The studies explored the behaviour of gas bubbles in PDMS-based microchannels under different flow properties like gas bubble behaviour in low capillary number microchannels; 26 roles of the cell-free layer and cell local concentration in the persistence of the bubble; 25 impacts of the T-junction on bubble break-up; 27 flow of blood cells around the bubble in PDMS-fabricated blood vessels; 28 and use of microfluidic devices for studying cellular responses to gas embolism. 29 Advances in microfluidic technology facilitated novel ways of studying complex physiochemical and biological phenomena in live tissues. 30,31 Microfluidic devices are often fabricated using polydimethylsiloxane (PDMS), 32 including those for studies related to gas embolism.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of air bubble behaviour after gas embolism events induced in a microfluidic network mimicking microvasculature

Mardanpour,

Sudalaiyadum Perumal,

Mahmoodi

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of air bubble behaviour after gas embolism events induced in a microfluidic network mimicking microvasculature

Mardanpour,

Sudalaiyadum Perumal,

Mahmoodi

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

“…Microfluidic chips provide a microenvironment conducive to cell growth, closely emulating physiological conditions within the human body. This characteristic renders them invaluable for advancing research in the realm of cellular science. − Microfluidic chips are comprised of a cover and a substrate, with the prevailing choice for the material of both the cover and substrate being poly(dimethylsiloxane) (PDMS) due to its advantageous characteristics: biocompatibility, gas permeability, optical transparency, facile microstructure molding, ease of bonding, notable chemical resistance, and cost-effectiveness. − However, the inherent hydrophobic nature of PDMS has been demonstrated as a factor contributing to compromised cell adhesion. − The amelioration of hydrophobicity within microchannels of PDMS chip can be achieved through modification with extracellular matrix proteins, such as fibronectin, collagen, and laminin. − However, in the context of long-term culture, the dissociation of these proteins may occur, resulting in the aggregation and detachment of cells. , Furthermore, hydrophilic treatments by this method tend to lose effectiveness within a few days, thus limiting their utility for extended culture periods or devices requiring prestorage before use. Coating procedures also escalated the experimental costs. − …”

Section: Introductionmentioning

confidence: 99%

Composite Microfluidic Petri Dish-Chip (MPD-Chip) without Protein Coating for 2D Cell Culture

Yin,

Lu,

Liu

et al. 2023

Langmuir

View full text Add to dashboard Cite

Hydrophilicity is a requisite attribute for the 2D cell culture substrate's surface, facilitating cell adhesion and spreading. Conventional poly(dimethylsiloxane) (PDMS) microfluidic chips necessitate protein coatings to enhance hydrophilicity; however, this approach is afflicted by issues of transient efficacy, interference with cell analysis, and high costs. This paper presents a protein-free microfluidic chip, termed a "microfluidic Petri dish-chip (MPD-chip)", integrating PDMS as the cover and a tissue culture-treated (TC-treated) Petri dish as the substrate. Microstructures are hot-embossed onto the Petri dish substrate using a silicon mold. This meticulous replication process serves to establish stable flow field dynamics within the chip. A simplified method for irreversible bonding, utilizing plasma activation and silylation, is proposed for affixing the PDMS cover onto the microstructured Petri dish substrate. The prepared composite chip exhibits remarkable tightness, boasting a notable bond strength of 2825 kPa. Furthermore, the composite microfluidic chip demonstrates the capability to withstand flow velocities of at least 200 μL/min, effectively meeting the required injection standards for both cell suspension and culture medium. SH-SY5Y and HeLa cells are cultured dynamically in the MPD-chip and control groups. Outcomes encompassing normalized cell density, cell adhesion area, and cell viability metrics unequivocally highlight the superiority of the MPD-chip in facilitating long-term two-dimensional (2D) cell cultures.

show abstract

“…48 Despite the numerous clinical works concentrating on mitigating surgery-derived or occupationally-derived pathological effects of gas embolism, 26,41,[68][69][70] only a few studies focused on mimicking gas embolism in microfluidic systems. 48,55,56,71,72 However, most of these studies focused on embolism in large blood vessels, e.g., 100 µm, or used blood flow rates or air pressures that are less biologically relevant. Also, many more questions regarding the impact of real-life parameters on gas embolism genesis are waiting for answers, such as: What is the effect of micro-vessel diameter (if less than 100 µm) on the genesis of gas embolism?…”

Section: Introductionmentioning

confidence: 99%

<em></em> <i>In Vitro</i> Investigation of Gas Embolism in Microfluidic Networks Mimicking Microvasculature

Mardanpour¹,

Perumal²,

Mahmoodi³

et al. 2022

Preprint

View full text Add to dashboard Cite

Gas embolism is a medical condition leading to the blockage of blood flow in the microvasculature by gas bubbles. While reported as rare, gas embolism often has devastating, fatal physiological consequences. Despite this acute importance, the genesis and evolution of air bubbles in blood vessels under different physiological conditions, such as blood viscosity and blood flow rate, is still understudied, largely because of difficult experimentation and in situ visualization. The objective of this work was to study the gas embolism phenomenon in vitro, using a microfluidic system that mimicked the architecture of microvasculature. The microfluidic systems comprised linear channels with two different air inlet types, namely, T- and Y-junctions with three different widths (20 µm, 40 µm, and 60 µm), and a 30 µm width honeycombed network with three bifurcation angles (30°, 60°, and 90°). Three synthetic liquids equivalent to 0%, 20%, and 46% hematocrit that mimicked the physiological blood viscosity and hematocrit concentrations were used. Our results show that: (i) 20 µm and 40 µm width channels had an elevated risk of gas embolism due to wide fluctuations in the total slug sizes; (ii) the resistance to the flow of air bubbles increased with the increase in the equivalent concentration of hematocrit; (iii) gas bubbles causing blockages and dampening of the flow velocity were frequently observed in 20 µm channels, and lastly (iv) increased risk of gas embolism was observed in the honeycomb architecture with 60° and 30° bifurcations. This work suggests that in vitro experimentation using microfluidic devices with microvascular tissue-like structures opens the possibility of studying this medical condition with high reproducibility and impacts the fact-based medical guidelines for preventing or mitigating iatrogenic occurrences.

show abstract

An integrated microfluidic device for studying controllable gas embolism induced cellular responses

Cited by 5 publications

References 59 publications

Investigation of air bubble behaviour after gas embolism events induced in a microfluidic network mimicking microvasculature

Investigation of air bubble behaviour after gas embolism events induced in a microfluidic network mimicking microvasculature

Composite Microfluidic Petri Dish-Chip (MPD-Chip) without Protein Coating for 2D Cell Culture

<em></em> <i>In Vitro</i> Investigation of Gas Embolism in Microfluidic Networks Mimicking Microvasculature

Contact Info

Product

Resources

About