An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells

Kamei, Ken‐ichiro; Guo, Shuling; Yu, Zeta Tak For; Takahashi, Hiroyuki; Gschweng, Eric H.; Suh, Carol Y.; Wang, Xiaopu; Tang, Jinghua; McLaughlin, Jami; Witte, Owen N.; Lee, Ki‐Bum; Tseng, Hsian‐Rong

doi:10.1039/b809105f

Cited by 96 publications

(82 citation statements)

References 39 publications

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“…[172][173][174] For example, an integrated microfluidic system with six individual cell-culture chambers was reported. 172 It allows for reproducible, quantitative, parallel hESC culture and multiparameter analyses in sequence. Several pluripotency markers for immunoassays were performed to confirm the pluripotent ability of hESC colonies.…”

Section: E Integrated Microsystems For Stem Cell Applicationsmentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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Microfluidic techniques have been recently developed for cell-based assays. In microfluidic systems, the objective is for these microenvironments to mimic in vivo surroundings. With advantageous characteristics such as optical transparency and the capability for automating protocols, different types of cells can be cultured, screened, and monitored in real time to systematically investigate their morphology and functions under well-controlled microenvironments in response to various stimuli. Recently, the study of stem cells using microfluidic platforms has attracted considerable interest. Even though stem cells have been studied extensively using bench-top systems, an understanding of their behavior in in vivo-like microenvironments which stimulate cell proliferation and differentiation is still lacking. In this paper, recent cell studies using microfluidic systems are first introduced. The various miniature systems for cell culture, sorting and isolation, and stimulation are then systematically reviewed. The main focus of this review is on papers published in recent years studying stem cells by using microfluidic technology. This review aims to provide experts in microfluidics an overview of various microfluidic systems for stem cell research.

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Section: E Integrated Microsystems For Stem Cell Applicationsmentioning

confidence: 99%

Stem cells in microfluidics

Lin²,

Lee³

2011

Biomicrofluidics

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“…The medium can be precisely controlled at the microscale level with this system. In addition, this system can combine with microscope to automatically monitor cell growth for a long time and to dynamically detect the cell growth in a real time manner by setting the operating software [37]. In the co-culture of human embryonic stem cells (hESC), PA6 stromal cells and separated nerve plexus structure, neurospheres can form in the medium [38].…”

Section: Microfluidics Lab On Chipmentioning

confidence: 99%

Application of Bioreactor in Stem Cell Culture

Zhang¹,

Wang²,

Pong³

et al. 2017

JBiSE

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Stem cells (SCs), the undifferentiated biological cells, have the infinite capacity to self-renew and the pluripotent ability to differentiate. SCs and their derived products offer great promise for biomedical applications such as cell therapy, tissue engineering, regenerative medicine and drug screening. However, the clinical applications of SCs require a large amount of SCs with high quality and the number of SCs from their tissue resources is very limited. Large-scale expansion is needed to generate homogeneous SCs with good biological characteristics for clinical application. This necessitates a bioreactor system to provide controllable and stable conditions for stem cell (SC) culture. Traditional methods of bioreactor for maintenance and expansion of cells rely on two-dimensional (2-D) culture techniques, leading to loss self-renewal ability and differentiation capacity upon long-term culture. New approaches for SC expansion with bioreactor employ three-dimensional (3-D) cell growth to mimic their environment in vivo. In this review, we summarize the application of bioreactors in SC culture.

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“…An integrated microfluidic platform for the culturing of human embryonic stem cells has been reported [225]. Here, the microfluidic device comprises a serpentine microchannel which facilitate the prescreening of dissociated hESC clusters and six individually addressable chambers.…”

Section: Aspergillus Ochraceusmentioning

confidence: 99%