An Integrated Microfluidic Biosensing System Based on a Versatile Valve and Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Salmonella typhimurium

Abstract

Detecting foodborne pathogens on-site is crucial for ensuring food safety, necessitating the development of rapid, cost-effective, highly sensitive, and portable devices. This paper presents an integrated microfluidic biosensing system designed for the rapid and sensitive detection of Salmonella typhimurium (S. typhimurium). The biosensing system comprises a microfluidic chip with a versatile valve, a recombinase polymerase amplification (RPA) for nucleic acid detection, and a customized real-time fluorescence detection system. The versatile valve combines the functions of an active valve and a magnetic actuation mixer, enabling on-demand mixing and controlling fluid flow. Quantitative fluorescence is processed and detected through a custom-built smartphone application. The proposed integrated microfluidic biosensing system could detect Salmonella at concentrations as low as 1.0 × 102 copies/µL within 30 min, which was consistent with the results obtained from the real-time quantitative polymerase chain reaction (qPCR) tests. With its versatile valve, this integrated microfluidic biosensing system holds significant potential for on-site detection of foodborne pathogens.

A One-Pot Convenient RPA-CRISPR-Based Assay for Salmonella enterica Serovar Indiana Detection

“…Recently, the RPA method has been used for the detection of Salmonella enterica [ 15 ]. Moreover, Yan Jin and colleagues have developed a RPA-based platform for Salmonella Typhimurium detection ( S. Typhimurium), which could work at concentrations as low as 1.0 × 10 2 copies/µL within 30 min [ 34 ]. However, efforts are still needed to eliminate the undesired nonspecific amplification and false-positive results.…”

Ensuring food safety: Microfluidic‐based approaches for the detection of food contaminants

“…In another novel study, a PDMS microfluidic chip was adhered to a glass substrate and implanted with a versatile valve for liquid mixing. 66 This chip displayed the detection of Salmonella typhimurium as low as 1.0 × 102 copies/µL within 30 min, offering significantly simplified and quicker on‐site detection of Salmonella compared to PCR‐based methods. Furthermore, paper microfluidic devices developed within the past few years have demonstrated the detection of L. monocytogenes, Campylobacter jejuni, S. aureus and Vibrio parahaemolyticus .…”

“…(Reproduced with permission ,72 Copyright 2021, Microchemical Journal).represents the most ubiquitous foodborne pathogen. In another novel study, a PDMS microfluidic chip was adhered to a glass substrate and implanted with a versatile valve for liquid mixing 66. This chip displayed the detection of Salmonella typhimurium as low as 1.0 × 102 copies/µL within 30 min, offering significantly simplified and quicker on-site detection of Salmonella compared to PCR-based methods.…”