An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities

Abstract

This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low-volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Microfluidic components have been developed and integrated to construct a microfluidic biochemical detection system. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and electrochemical sensor on a glass microfluidic motherboard. Total time required for an immunoassay was less than 20 min including sample incubation time, and sample volume wasted was less than 50 microl during five repeated assays. Fast and low-volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to the microfluidic system. Such a magnetic bead-based biochemical detection system, described in this paper, can be applied to protein analysis systems.

Magnetic bead handling on-chip: new opportunities for analytical applications

“…The group of Ahn reported several of such integrated bead separation systems (Ahn et al 1996;Choi et al 2000Choi et al , 2001. Such devices can be used, for example, for miniaturized cell sorting, where the magnetic beads are used as a magnetic ''label'' for actuation, or in a miniaturized bio-reaction system, where the magnetic beads play the role of the carrier substrate in an assay (Choi et al 2000(Choi et al , 2002. Figure 8 is a schematic diagram of a sandwich immuno-assay that uses magnetic separation.…”

“…A few nanograms of mRNA could be captured per microgram of total RNA. Choi et al (2002) reported on the development and characterization of an integrated microfluidic biochemical detection system for fast and low-volume immunoassays using magnetic beads (Choi et al 2002). First, antibody-labeled polystyrene magnetic beads were separated from a liquid solution by a set of planar coils.…”

PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays

“…The boom in microfluidics, which began about 20 years ago, brought with it ideas about incorporating magnetic particles into various microfluidic devices [6][7][8][9] . This offers additional benefits compared to batchtype processes, such as reduced sample and reagent volumes, faster reaction times, the possibility to engineer the design of a chip to control particle mobility, and integration of various processes into a unified system 5 .…”

Construction and characterisation of a modular microfluidic system: coupling magnetic capture and electrochemical detection

“…Choi et al used interdigitated microelectrode structures operated under generatorcollector mode in an example close to our approach (Choi et al, 2002). More recently, a system was reported where the magnetic particles are elegantly immobilised by means of an array of NiFe permalloy blocks (Do and Ahn, 2008).…”

“…Magnetic particles and amperometry have been combined in the past for (bio)electroanalytical purposes (Choi et al, 2002, Do and Ahn, 2008, Farrell et al, 2004, Gabig-Ciminska et al, 2004, Goral et al, 2006, Llopis et al, 2009) and several literature reports describe systems where particles are directly immobilised 5 on a channel wall opposite the electrodes (Choi et al, 2002, Do andAhn, 2008).…”