An Integrated Genetic Linkage Map for Almond Based on Rapd, Issr, SSR and Morphological Markers

Gregory, D.; Sedgley, Margaret; Wirthensohn, M.; Arús, Pere; Kaiser, Brendan W.; Collins, Graham

doi:10.17660/actahortic.2005.694.7

Cited by 4 publications

(6 citation statements)

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“…Linkage maps based on intraspecific crosses of almond have been reported for the crosses of 'Ferragnes' × 'Tuono' (F × T) [ 15 - 17 ], 'Felisia' × 'Bertina' (F × B) [ 13 , 18 ], 'R1000' × 'Desmayo Largueta' (RxD) [ 4 ], and 'Nonpareil × Lauranne' (N × L) [ 20 , 21 ]. While F × T and F × B maps consisted mostly of RFLP and RAPD markers, the RxD map contains 56 SSR markers with less density across the genome.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, a saturated map containing additional SSR markers is warranted in almond intraspecific crosses. The N × L map was initiated with RAPDs, ISSRs and the small numbers of SSR, and a sparse integrated map was subsequently produced [ 20 ]. Using high resolution melting curve analysis [ 32 ], Wu et al mapped 12 gene-anchored SNPs on six linkage groups plus the addition of more SSR to the map [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, more mapping populations and saturated maps are required to assist broader assessment of the almond traits and gene discovery especially under different environments and management systems. In Australia, a genetic linkage mapping program was initiated by Gregory [ 19 ] in a 'Nonpareil' × 'Lauranne' cross (N × L), but the integrated map with mainly RAPDs and ISSRs was sparse and further saturation was desirable [ 20 ]. Recently, Wu et al (2009) reported that 12 SNP-anchored genes were mapped on six linkage groups in the same population with a higher map density [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we developed a moderately saturated linkage map by adding SSRs and SNPs to the N × L F1 map constructed by Gregory et al (2005) [ 20 ]. The map was compared with the Prunus T × E reference map to demonstrate high synteny and colinearity between the N × L and the reference map.…”

Section: Introductionmentioning

confidence: 99%

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Construction of an almond linkage map in an Australian population Nonpareil × Lauranne

et al. 2010

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BackgroundDespite a high genetic similarity to peach, almonds (Prunus dulcis) have a fleshless fruit and edible kernel, produced as a crop for human consumption. While the release of peach genome v1.0 provides an excellent opportunity for almond genetic and genomic studies, well-assessed segregating populations and the respective saturated genetic linkage maps lay the foundation for such studies to be completed in almond.ResultsUsing an almond intraspecific cross between 'Nonpareil' and 'Lauranne' (N × L), we constructed a moderately saturated map with SSRs, SNPs, ISSRs and RAPDs. The N × L map covered 591.4 cM of the genome with 157 loci. The average marker distance of the map was 4.0 cM. The map displayed high synteny and colinearity with the Prunus T × E reference map in all eight linkage groups (G1-G8). The positions of 14 mapped gene-anchored SNPs corresponded approximately with the positions of homologous sequences in the peach genome v1.0. Analysis of Mendelian segregation ratios showed that 17.9% of markers had significantly skewed genotype ratios at the level of P < 0.05. Due to the large number of skewed markers in the linkage group 7, the potential existence of deleterious gene(s) was assessed in the group. Integrated maps produced by two different mapping methods using JoinMap® 3 were compared, and their high degree of similarity was evident despite the positional inconsistency of a few markers.ConclusionsWe presented a moderately saturated Australian almond map, which is highly syntenic and collinear with the Prunus reference map and peach genome V1.0. Therefore, the well-assessed almond population reported here can be used to investigate the traits of interest under Australian growing conditions, and provides more information on the almond genome for the international community.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Construction of an almond linkage map in an Australian population Nonpareil × Lauranne

et al. 2010

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“…Twelve almond putative genes corresponding to Prunus unigenes were mapped in the N £ L linkage map consisting of RAPD, ISSR and microsatellite markers (Gregory et al 2005;Tavassolian 2008). These genes were located in six of the eight linkage groups: three each on linkage groups 1 and 6, two each on linkage group 2 and 7, and one each on linkage groups 3 and 8 ( Fig.…”

Section: Mapping Of the Genes In N £ L Linkage Groupsmentioning

confidence: 99%

Mapping SNP-anchored genes using high-resolution melting analysis in almond

Tavassolian

Rabiei

et al. 2009

Mol Genet Genomics

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Peach and almond have been considered as model species for the family Rosaceae and other woody plants. Consequently, mapping and characterisation of genes in these species has important implications. High-resolution melting (HRM) analysis is a recent development in the detection of SNPs and other markers, and proved to be an efficient and cost-effective approach. In this study, we aimed to map genes corresponding to known proteins in other species using the HRM approach. Prunus unigenes were searched and compared with known proteins in the public databases. We developed single-nucleotide polymorphism (SNP) markers, polymorphic in a mapping population produced from a cross between the cloned cultivars Nonpareil and Lauranne. A total of 12 SNP-anchored putative genes were genotyped in the population using HRM, and mapped to an existing linkage map. These genes were mapped on six linkage groups, and the predicted proteins were compared to putative orthologs in other species. Amongst those genes, four were abiotic stress-responsive genes, which can provide a starting point for construction of an abiotic resistance map. Two allergy and detoxification related genes, respectively, were also mapped and analysed. Most of the investigated genes had high similarities to sequences from closely related species such as apricot, apple and other eudicots, and these are putatively orthologous. In addition, it was shown that HRM can be an effective means of genotyping populations for the purpose of constructing a linkage map. Our work provides basic genomic information for the 12 genes, which can be used for further genetic and functional studies.

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Almond

Oliveira

Miguel

Costa

2008

Compendium of Transgenic Crop Plants

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Almond evolved in the deserts and central slopes of central and southwest Asia (present China and Iran) and was later distributed by many temperate areas all around the word. Almond is a nut fruit of high economical importance, used in various food products and exploited for its oil in pharmaceutical industry and cosmetics. Being very close to peach and other Prunus species, almond can easily hybridize with these close relatives, which may have led to gene exchange in both directions. Self‐fertility and late blooming are among the most important breeding traits. Almond breeding is presently being supported by biotechnology tools, to track the self‐fertility allele in the progeny among other traits that are being mapped. Although there is not an obvious need for genetic engineering tools in almond breeding, engineering strategies have been developed and transgenic plants were regenerated using Agrobacterium ‐mediated gene transfer. This, however, is still only applicable to a couple of genotypes. At present, the most important use of genetic engineering tools in almond seems to be gene functional studies.

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An Integrated Genetic Linkage Map for Almond Based on Rapd, Issr, SSR and Morphological Markers

Cited by 4 publications

References 0 publications

Construction of an almond linkage map in an Australian population Nonpareil × Lauranne

Construction of an almond linkage map in an Australian population Nonpareil × Lauranne

Mapping SNP-anchored genes using high-resolution melting analysis in almond

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