An integrated electrodialysis-biocatalysis-spray-drying process for efficient recycling of keratin acid hydrolysis industrial wastewater

Liu, Junzhong; Wu, Siping; Lü, Yang; Liu, Qian; Jiao, Qingcai; Wang, Xiuzhen; Zhang, Hongjuan

doi:10.1016/j.cej.2016.05.046

Cited by 25 publications

(6 citation statements)

References 30 publications

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“…Traditional thermal hydrolysis and chemical treatment produced poor-quality products and released sulfur and ammonia waste gas, which pollute the environment (da Silva, 2018; J. Liu et al, 2016). Recently, keratinase has shown potential in the field of keratin waste management, such as converting feathers into soluble proteins, peptides and amino acids (Z.…”

Section: Introductionmentioning

confidence: 99%

Engineered pro‐peptide enhances the catalytic activity of keratinase to improve the conversion ability of feather waste

Peng

Zhang

Song

et al. 2021

Biotech & Bioengineering

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Keratinase is an attractive industrial enzyme that can specifically catalyze keratin waste to obtain value-added products. A challenge to the application of keratinase is improving catalytic capacity to achieve efficient hydrolysis. In this study, we effectively expressed the keratinase gene from Bacillus licheniformis BBE11-1 in Bacillus subtilis WB600 based on pro-peptide engineering. Partial deletion of the pro-peptide sequence and the substitution of amino acid at the pro-peptide cleavage site (P1) suggested that the "chaperone effect" and "cleavage efficiency" of the pro-peptide determine the activity of the mature enzyme. Subsequently, seven target sites that can increase the activity of the mature enzyme by 16%-66% were obtained through the multiple sequence alignment of pro-peptides and site-directed mutation. We further performed combinatorial mutations at six sites based on the design principle of three-codon saturation mutations and obtained mutant 2-D12 (236.8 KU/mg) with a mature enzyme activity of 186% of the original (127.6 KU/mg). Finally, continuous fermentation was carried out in a 5-L bioreactor for 22 h, and the activity of the 2-D12 mature enzyme was increased to 391.6 KU/mg. Most importantly, 2-D12 could degrade more than 90% of feather waste into amino acids and peptides within 12 h with the aid of sulfite.

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Section: Introductionmentioning

confidence: 99%

Engineered pro‐peptide enhances the catalytic activity of keratinase to improve the conversion ability of feather waste

Peng

Zhang

Song

et al. 2021

Biotech & Bioengineering

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“…Methods that have been developed for keratin hydrolysis primarily include physical (pressurized hydrolysis, puffing, and microwaving) or chemical (acid and alkali). , However, these methods present limitations, such as high energy consumption during the production process and high levels of damage to the reaction products . On the other hand, microbial degradation of keratin has advantages of being environmentally friendly and the ability of producing high-value productsalbeit at suboptimal efficiency .…”

Section: Introductionmentioning

confidence: 99%

Keratin Waste Recycling Based on Microbial Degradation: Mechanisms and Prospects

Peng

Zhang

et al. 2019

ACS Sustainable Chem. Eng.

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The severe shortage of protein resources has motivated researchers to seek cheap and sustainable proteins and to produce new functional materials in an environmentally friendly way. Keratin is a ubiquitous and stubborn protein that is attractive for sustainability but difficult to recycle. Structurally, keratin is characterized by a large amount of disulfide bonds, hydrogen bonds and hydrophobic forces and results in considerable hardness. Biorecycling of keratin is more promising than traditional processors because of the green production process, rich products and higher product value. Microbial strains capable of degrading keratin are constantly being developed. However, the mechanism of keratin decomposition by these microorganisms is not fully elucidated, which greatly hinders the sustainable use of keratin waste. In this review, we summarize the underlying mechanisms involved in microbial decomposition of keratin and attempt to discuss prospects and future directions in conjunction with systems biology strategies.

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“…ED technologies have been widely studied for production of organic acids, with development for some industrial applications [19,308]. New opportunities are derived from the recovery of organic acids from wastewaters via ED [309][310][311][312][313][314][315], or BMED [316][317][318] or both [319,320], including systems combined with biotechnologies.…”

Section: Effluents With Organic Mattermentioning

confidence: 99%

Electrodialysis Applications in Wastewater Treatment for Environmental Protection and Resources Recovery: A Systematic Review on Progress and Perspectives

et al. 2020

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This paper presents a comprehensive review of studies on electrodialysis (ED) applications in wastewater treatment, outlining the current status and the future prospect. ED is a membrane process of separation under the action of an electric field, where ions are selectively transported across ion-exchange membranes. ED of both conventional or unconventional fashion has been tested to treat several waste or spent aqueous solutions, including effluents from various industrial processes, municipal wastewater or salt water treatment plants, and animal farms. Properties such as selectivity, high separation efficiency, and chemical-free treatment make ED methods adequate for desalination and other treatments with significant environmental benefits. ED technologies can be used in operations of concentration, dilution, desalination, regeneration, and valorisation to reclaim wastewater and recover water and/or other products, e.g., heavy metal ions, salts, acids/bases, nutrients, and organics, or electrical energy. Intense research activity has been directed towards developing enhanced or novel systems, showing that zero or minimal liquid discharge approaches can be techno-economically affordable and competitive. Despite few real plants having been installed, recent developments are opening new routes for the large-scale use of ED techniques in a plethora of treatment processes for wastewater.

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An integrated electrodialysis-biocatalysis-spray-drying process for efficient recycling of keratin acid hydrolysis industrial wastewater

Cited by 25 publications

References 30 publications

Engineered pro‐peptide enhances the catalytic activity of keratinase to improve the conversion ability of feather waste

Engineered pro‐peptide enhances the catalytic activity of keratinase to improve the conversion ability of feather waste

Keratin Waste Recycling Based on Microbial Degradation: Mechanisms and Prospects

Electrodialysis Applications in Wastewater Treatment for Environmental Protection and Resources Recovery: A Systematic Review on Progress and Perspectives

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