An integrated electro-optical biosensor system for rapid, low-cost detection of bacteria

Petrovszki, Dániel; Valkai, Sándor; Gora, Evelin; Tanner, Martin A.; Bányai, Anita; Fürjes, Péter; Dér, András

doi:10.1016/j.mee.2021.111523

Cited by 27 publications

(20 citation statements)

References 31 publications

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“…The sensor devices used in this study were fabricated and improved based on the construction of the integrated optical MZI interferometer biosensor described in detail in a previous study of our research group [ 22 ]. The waveguide structure was made by direct laser writing technique (μPG-101 machine,

, Heidelberg Instruments GmbH, Heidelberg, Germany), as was previously described [ 23 ]. To improve the waveguiding performance of the photopolymer structure, the glass substrate was covered with an adhesion promoter layer (Surpass 3000, MicroResist Technology GmbH, Berlin, Germany) by dip coating, following the protocol of the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

“…As in our previous study [ 22 ], heating was used for setting the bias point prior to the measurements by applying DC voltage on a substrate-bound 20 nm thick gold microheater structure. To improve its adherence to the substrate 10 nm thick Cr was deposited followed by the deposition of the desired gold layer, as was described in one of our latest works [ 23 ]. The heater was placed to leave enough space for dissipation, so that the heating of the fluid sample and the measuring arm of the interferometer was avoided, while stabile bias point tuning was achieved.…”

Section: Methodsmentioning

confidence: 99%

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Penetration of the SARS-CoV-2 Spike Protein across the Blood–Brain Barrier, as Revealed by a Combination of a Human Cell Culture Model System and Optical Biosensing

et al. 2022

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Since the outbreak of the global pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), several clinical aspects of the disease have come into attention. Besides its primary route of infection through the respiratory system, SARS-CoV-2 is known to have neuroinvasive capacity, causing multiple neurological symptoms with increased neuroinflammation and blood–brain barrier (BBB) damage. The viral spike protein disseminates via circulation during infection, and when reaching the brain could possibly cross the BBB, which was demonstrated in mice. Therefore, its medical relevance is of high importance. The aim of this study was to evaluate the barrier penetration of the S1 subunit of spike protein in model systems of human organs highly exposed to the infection. For this purpose, in vitro human BBB and intestinal barrier cell–culture systems were investigated by an optical biosensing method. We found that spike protein crossed the human brain endothelial cell barrier effectively. Additionally, spike protein passage was found in a lower amount for the intestinal barrier cell layer. These observations were corroborated with parallel specific ELISAs. The findings on the BBB model could provide a further basis for studies focusing on the mechanism and consequences of spike protein penetration across the BBB to the brain.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Penetration of the SARS-CoV-2 Spike Protein across the Blood–Brain Barrier, as Revealed by a Combination of a Human Cell Culture Model System and Optical Biosensing

et al. 2022

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“…From this brief overview on the techniques for the detection and analysis of bacteria, it is clear that a single interrogation technique is not sufficient for a full assessment of the antibiotic susceptibility on planktonic bacteria and, in particular, on bacterial biofilms. Multiple approaches should be used in parallel, leading to a multiparameter approach [35,36].…”

Section: Techniques For the Bacteria Detection And Analysismentioning

confidence: 99%

Novel Micro-Nano Optoelectronic Biosensor for Label-Free Real-Time Biofilm Monitoring

et al. 2021

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According to the World Health Organization forecasts, AntiMicrobial Resistance (AMR) is expected to become one of the leading causes of death worldwide in the following decades. The rising danger of AMR is caused by the overuse of antibiotics, which are becoming ineffective against many pathogens, particularly in the presence of bacterial biofilms. In this context, non-destructive label-free techniques for the real-time study of the biofilm generation and maturation, together with the analysis of the efficiency of antibiotics, are in high demand. Here, we propose the design of a novel optoelectronic device based on a dual array of interdigitated micro- and nanoelectrodes in parallel, aiming at monitoring the bacterial biofilm evolution by using optical and electrical measurements. The optical response given by the nanostructure, based on the Guided Mode Resonance effect with a Q-factor of about 400 and normalized resonance amplitude of about 0.8, allows high spatial resolution for the analysis of the interaction between planktonic bacteria distributed in small colonies and their role in the biofilm generation, calculating a resonance wavelength shift variation of 0.9 nm in the presence of bacteria on the surface, while the electrical response with both micro- and nanoelectrodes is necessary for the study of the metabolic state of the bacteria to reveal the efficacy of antibiotics for the destruction of the biofilm, measuring a current change of 330 nA when a 15 µm thick biofilm is destroyed with respect to the absence of biofilm.

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“…A similar report presented a "biosensing" system, which is reported to be able to detect bacterial cells in low-volume fluid samples [37]. In this method, similarly to the previous one, the scientists have combined a "dielectrophoretic cell-collecting technique with evanescent-field sensing" [37].…”

Section: Introduction 1applications and Optical Spectra In Biological...mentioning

confidence: 99%

“…The addition of the electrical approach allowed the concurrent study of the developing phases of the bacterial biofilm, and the impedance changes provided insight to the evaluation of the biofilm's growth and maturation [36]. A similar report presented a "biosensing" system, which is reported to be able to detect bacterial cells in low-volume fluid samples [37]. In this method, similarly to the previous one, the scientists have combined a "dielectrophoretic cell-collecting technique with evanescent-field sensing" [37].…”

Section: Introduction 1applications and Optical Spectra In Biological...mentioning

confidence: 99%

Physical and Methodological Perspectives on the Optical Properties of Biological Samples: A Review

et al. 2021

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The optical properties of biological systems can be measured by imaging and microscopy methodologies. The use of X-rays, γ-radiation and electron microscopy provides information about the contents and functions of the systems. The need to develop imaging methods and analyses to measure these optical properties is increasing. On the other hand, biological samples are easily penetrated by a high-energy input, which has revolutionized the field of tissue optical properties and has now reached a point where light can be applied in the diagnosis and treatment of diseases. To this end, developing methodologies would allow the in-depth study of optical properties of tissues. In the present work, we review the literature focusing on optical properties of biological systems and tissues. We have reviewed the literature for related articles on biological samples’ optical properties. We have reported on the theoretical concepts and the applications of Monte Carlo simulations in the studies of optical properties of biological samples. Optical properties of biological samples are of paramount importance for the understanding of biological samples as well as for their applications in disease diagnosis and therapy.

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An integrated electro-optical biosensor system for rapid, low-cost detection of bacteria

Cited by 27 publications

References 31 publications

Penetration of the SARS-CoV-2 Spike Protein across the Blood–Brain Barrier, as Revealed by a Combination of a Human Cell Culture Model System and Optical Biosensing

Penetration of the SARS-CoV-2 Spike Protein across the Blood–Brain Barrier, as Revealed by a Combination of a Human Cell Culture Model System and Optical Biosensing

Novel Micro-Nano Optoelectronic Biosensor for Label-Free Real-Time Biofilm Monitoring

Physical and Methodological Perspectives on the Optical Properties of Biological Samples: A Review

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