An Integrated Chromatographic Strategy for the Large-Scale Extraction of Ergosterol from Tulasnellaceae sp.

Abstract

A reliable chromatographic strategy is crucial for the extraction of target compounds from natural sources as it is related to the preparation efficiency, as well as the purity of the compounds. In this study, medium-pressure normal-phase liquid chromatography and high-pressure reverse-phase liquid chromatography were combined to prepare and purify ergosterol from Tulasnellaceae sp. of Gymnadenia orchidis. First, Tulasnellaceae sp. was extracted three times (2.0 L and 2 h each time) with ethyl acetate, and the 6.0 L of extract solution was concentrated under reduced pressure to yield 2.2 g of crude sample. Then, the crude sample was pretreated utilizing silica gel medium-pressure liquid chromatography to enrich the target ingredient (586.0 mg). Finally, high-pressure reversed-phase liquid chromatography was used to purify the target compound, and the compound was characterized as ergosterol (purity > 95%) using spectral data. Overall, the simple and reproducible integrated chromatographic strategy developed in this study has the potential for the large-scale purification of steroids for laboratory and even industrial research. To the best of our knowledge, this is also the first report of ergosterol in Tulasnellaceae sp.

Prediction of Historical, Current, and Future Configuration of Tibetan Medicinal Herb Gymnadenia orchidis Based on the Optimized MaxEnt in the Qinghai–Tibet Plateau

“…It possesses significant medicinal value, including the treatment of lung deficiency, bleeding, and cough. It also plays a crucial role in the combined treatment of type 2 diabetes (T2DM), where its tuberous root and pumpkin seeds are utilized [ 8 ]. Relevant studies have indicated a positive correlation between the compounds found in G. orchidis and its growth age.…”

“…However, research on G. orchidis has primarily focused on its pharmacological actions, with limited publications on predicting its potential suitable zones [ 10 ]. This knowledge gap is concerning given the species’ longer growth cycle and reliance on wild resources, making it increasingly rare [ 8 , 11 ]. The wild resources of G. orchidis are continuously declining, with low production but increasing demand each year [ 12 ].…”

Prediction of historical, current and future configuration of Gymnadenia orchidis 1 based on the optimized MaxEnt in the Qinghai-Tibet Plateau

“…G. orchidis, known as "Wangla" in Tibetan medicine, possesses significant medicinal value, including the treatment of lung deficiency, bleeding, and cough (Wang et al 2022). It also plays a crucial role in the combined treatment of type 2 diabetes (T2DM), where its tuberous root and pumpkin seeds are utilized (Wang and Wang 2022). However, research on G. orchidis has primarily focused on its pharmacological actions, with limited publications on predicting its potential suitable zones (Subiah et al 2018).…”

“…However, research on G. orchidis has primarily focused on its pharmacological actions, with limited publications on predicting its potential suitable zones (Subiah et al 2018). This knowledge gap is concerning given the species' longer growth cycle and reliance on wild resources, making it increasingly rare (Wang and Wang 2022;Xi et al 2000). The wild resources of G. orchidis are continuously declining, with low production but increasing demand each year (Hisashi et al 2004).…”

“…Gymnosia orchidis (G. orchidis) is a vulnerable species (VU) belonging to the Gymnadenia genus in the Orchidaceae family, It holds the highest extinction risk among numerous plant species (Barbara et al 2004;luo et al 2013). G. orchidis, known as "Wangla" in Tibetan medicine, possesses significant medicinal value, including the treatment of lung deficiency, bleeding, and cough (Wang et al 2022). It also plays a crucial role in the combined treatment of type 2 diabetes (T2DM), where its tuberous root and pumpkin seeds are utilized (Wang and Wang 2022).…”