An integrated biochemical system for nitrate assimilation and nitric oxide detoxification in <i>Bradyrhizobium japonicum</i>

Cabrera, Juan J.; Salas, Ana; Torres, María J.; Bedmar, Eulogio J.; Richardson, David J.; Gates, Andrew J.; Delgado, Manuel Lorenzo

doi:10.1042/bj20150880

Cited by 37 publications

(64 citation statements)

References 61 publications

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“…Although, the biochemical basis for NO-formation during anaerobic bacterial respiration has been shown to result from NR-catalysed reduction of nitrite by Nar (Gilberthorpe & Poole, 2008;Rowley et al, 2012;Vine & Cole, 2011), to our knowledge, this is the first time where a combined nitrate assimilation/NO-detoxification system represents a novel method by which bacteria protect against cytoplasmic NO produced by NasC during anaerobic nitrate-dependent growth, where pathways for both respiratory denitrification and nitrate assimilation are active ( Fig. 12; Cabrera et al, 2016). These observations strongly suggest that in addition to denitrification, rhizobial nitrate assimilation might be another important source of NO and N 2 O in nodules.…”

Section: A New System Involved In No and N 2 O Metabolism In B Japonmentioning

confidence: 95%

“…This integrated system for NO detoxification and nitrate assimilation has been demonstrated to be another source of NO and probably to N 2 O. In fact, the importance of NasC not only in nitrate assimilation but also in NO production has been demonstrated (Cabrera et al, 2016). Although, the biochemical basis for NO-formation during anaerobic bacterial respiration has been shown to result from NR-catalysed reduction of nitrite by Nar (Gilberthorpe & Poole, 2008;Rowley et al, 2012;Vine & Cole, 2011), to our knowledge, this is the first time where a combined nitrate assimilation/NO-detoxification system represents a novel method by which bacteria protect against cytoplasmic NO produced by NasC during anaerobic nitrate-dependent growth, where pathways for both respiratory denitrification and nitrate assimilation are active ( Fig.…”

Section: A New System Involved In No and N 2 O Metabolism In B Japonmentioning

confidence: 96%

“…Similarly to other bacterial haemoglobins, Bjgb might reduce NO to N 2 O under anoxic free-living conditions or inside the nodules. In B. japonicum, the Bjgb is encoded in a gene cluster that also codes for a number of proteins with important roles in nitrate assimilation (Cabrera et al, 2016) including the large catalytic subunit of the assimilatory nitrate reductase (NasC), a major-facilitator superfamily-type nitrate/nitrite transporter, an FAD-dependent NAD(P)H oxidoreductase (Fig. 12).…”

Section: A New System Involved In No and N 2 O Metabolism In B Japonmentioning

confidence: 99%

“…1). Recent findings have proposed the involvement of the Salmonella enterica serovar Typhimurium membrane-bound respiratory nitrate reductase (Nar) (Gilberthorpe & Poole, 2008;Rowley et al, 2012) and the Bradyrhizobium japonicum assimilatory nitrate reductase (NasC) (Cabrera et al, 2016) in NO and N 2 O metabolism (Fig. 1).…”

Section: Introductionmentioning

confidence: 97%

“…Bjgb might detoxify NO to N 2 O in the absence of O 2 . Adapted from Cabrera, J. J., Salas, A.,Torres, M. J., Bedmar, E. J., Richardson, D. J., Gates, A. J., et al (2016). An integrated biochemical system for nitrate assimilation and nitric oxide detoxification in Bradyrhizobium japonicum.…”

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confidence: 99%

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Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Torres

Simon

Rowley

et al. 2016

Advances in Microbial Physiology

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Nitrous oxide (N2O) is an important greenhouse gas (GHG) with substantial global warming potential and also contributes to ozone depletion through photochemical nitric oxide (NO) production in the stratosphere. The negative effects of N2O on climate and stratospheric ozone make N2O mitigation an international challenge. More than 60% of global N2O emissions are emitted from agricultural soils mainly due to the application of synthetic nitrogen-containing fertilizers. Thus, mitigation strategies must be developed which increase (or at least do not negatively impact) on agricultural efficiency whilst decrease the levels of N2O released. This aim is particularly important in the context of the ever expanding population and subsequent increased burden on the food chain. More than two-thirds of N2O emissions from soils can be attributed to bacterial and fungal denitrification and nitrification processes. In ammonia-oxidizing bacteria, N2O is formed through the oxidation of hydroxylamine to nitrite. In denitrifiers, nitrate is reduced to N2 via nitrite, NO and N2O production. In addition to denitrification, respiratory nitrate ammonification (also termed dissimilatory nitrate reduction to ammonium) is another important nitrate-reducing mechanism in soil, responsible for the loss of nitrate and production of N2O from reduction of NO that is formed as a by-product of the reduction process. This review will synthesize our current understanding of the environmental, regulatory and biochemical control of N2O emissions by nitrate-reducing bacteria and point to new solutions for agricultural GHG mitigation.

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Section: A New System Involved In No and N 2 O Metabolism In B Japonmentioning

confidence: 95%

Section: A New System Involved In No and N 2 O Metabolism In B Japonmentioning

confidence: 96%

Section: A New System Involved In No and N 2 O Metabolism In B Japonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

mentioning

confidence: 99%

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Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Torres

Simon

Rowley

et al. 2016

Advances in Microbial Physiology

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Senescence of Medicago truncatula root nodules: NO balance

Blanquet

Bruand

Meilhoc

2019

The Model Legume Medicago Truncatula

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Dissecting the role of NtrC and RpoN in the expression of assimilatory nitrate and nitrite reductases in Bradyrhizobium diazoefficiens

López

Cabrera

Salas

et al. 2016

Antonie van Leeuwenhoek

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Bradyrhizobium diazoefficiens, a nitrogen-fixing endosymbiont of soybeans, is a model strain for studying rhizobial denitrification. This bacterium can also use nitrate as the sole nitrogen (N) source during aerobic growth by inducing an assimilatory nitrate reductase encoded by nasC located within the narK-bjgb-flp-nasC operon along with a nitrite reductase encoded by nirA at a different chromosomal locus. The global nitrogen two-component regulatory system NtrBC has been reported to coordinate the expression of key enzymes in nitrogen metabolism in several bacteria. In this study, we demonstrate that disruption of ntrC caused a growth defect in B. diazoefficiens cells in the presence of nitrate or nitrite as the sole N source and a decreased activity of the nitrate and nitrite reductase enzymes. Furthermore, the expression of narK-lacZ or nirA-lacZ transcriptional fusions was significantly reduced in the ntrC mutant after incubation under nitrate assimilation conditions. A B. diazoefficiens rpoN mutant, lacking both copies of the gene encoding the alternative sigma factor σ, was also defective in aerobic growth with nitrate as the N source as well as in nitrate and nitrite reductase expression. These results demonstrate that the NtrC regulator is required for expression of the B. diazoefficiens nasC and nirA genes and that the sigma factor RpoN is also involved in this regulation.

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An integrated biochemical system for nitrate assimilation and nitric oxide detoxification in Bradyrhizobium japonicum

Cited by 37 publications

References 61 publications

Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria

Senescence of Medicago truncatula root nodules: NO balance

Dissecting the role of NtrC and RpoN in the expression of assimilatory nitrate and nitrite reductases in Bradyrhizobium diazoefficiens

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