An integrated bienzyme glucose oxidase–fructose dehydrogenase–tetrathiafulvalene-3-mercaptopropionic acid–gold electrode for the simultaneous determination of glucose and fructose

Campuzano, Susana; Loaiza, Óscar A.; Pedrero, Marı́a; Villena, F. Javier Manuel de; Pingarrón, José M.

doi:10.1016/j.bioelechem.2003.10.019

Cited by 37 publications

(19 citation statements)

References 12 publications

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“…Self-assembled monolayers formed by the adsorption of mercaptopropionic acid (MPA) on gold have demonstrated recently their usefulness for the design of robust enzyme [13,14], and DNA [15] electrochemical biosensors. The exploitation of thiol based SAMs for immobilization of antibodies in immunosensing has also been previously reported [16].…”

Section: Introductionmentioning

confidence: 99%

Development of an Amperometric Immunosensor for the Quantification of Staphylococcus aureus Using Self‐Assembled Monolayer‐Modified Electrodes as Immobilization Platforms

et al. 2007

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Amperometric immunosensors for the detection and quantification of S. aureus using MPA self-assembled monolayer modified electrodes for the immobilization of the immunoreagents are reported. Two different immunosensor configurations were compared. A competitive mode, in which protein A-bearing S. aureus cells and antiRbIgG labeled with horseradish peroxidase (HRP) compete for the binding sites of RbIgG immobilized onto the 3-mercaptopropionic acid (MPA) modified electrode, was evaluated. Moreover, a sandwich configuration in which S. aureus cells were immobilized onto the MPA SAM, and RbIgG and antiRbIgG labeled with HRP were further linked to the electrode surface, was also tested. In both cases, TTF was used as the redox mediator of the HRP reaction with H 2 O 2 , and it was co-immobilized onto the MPA-modified gold electrode. After optimization of the working variables for both configurations, the analytical performance of the amperometric measurements carried out at 0.00 V (vs. Ag/ AgCl) showed that the competitive immunosensor exhibited a lower limit of detection (1.6 Â 10 5 S. aureus cells mL À1 ), as well as a better repeatability and reproducibility of the measurements.

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Section: Introductionmentioning

confidence: 99%

Development of an Amperometric Immunosensor for the Quantification of Staphylococcus aureus Using Self‐Assembled Monolayer‐Modified Electrodes as Immobilization Platforms

et al. 2007

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“…Both stability and the biological function of the enzyme mainly depend of the immobilization. Thus different enzyme immobilization techniques are possible [20], as adsorption [21], cross-linking [22,23], entrapment [24,25] or electropolymerization [26,27] are carried out in order to ensure the stability of the sensor. The present work describes the design of a glucose sensor, using a screen-printed ferrocyanide/carbon electrode (SPFCE).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Sensor Using Mediator‐Screen‐Printed Carbon Electrodes

et al. 2010

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A new glucose sensor was developed using screen-printed ferrocyanide/carbon electrodes. The ferrocyanide is included in the carbon ink of the commercial screen-printed electrode. The immobilization of enzymes glucose oxidase (Gox) and horseradish peroxidase (HRP) were carried out in a very easy way. An aliquot of 10 mL of a Gox/ HRP mixture was deposited on the electrode surface and left there until dried (approximately 1 h) at room temperature. The ferricyanide generated enzymatically was detected amperometrically applying a potential of À0.1 V (vs. Ag pseudo reference electrode). The sensor, so constructed, shows a good sensitivity to glucose (À2.12 mA/mM) with a slope deviation of AE 0.06 mA/mM and a linear range comprised between 0.05 and 1 mM of glucose, with a limit of detection of 0.025 mM. These sensors show good intersensor reproducibility and a high stability. When they are stored at 4 8C, no significant changes in the slope value of the glucose calibration plot were found after 3 months. Glucose was determined in real samples as honey, blood, drink for babies and glucosed drink with a great accuracy.

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“…It is also possible to measure glucose and fructose simultaneously by using a sample split valve leading to a glucose oxidase thermistor without the need for a separation step. However, simultaneous determination of glucose and fructose in the same sample by amperometry is difficult as both the sugars show amperometric signal at the detection potential used [19]. With respect to the life of the sensor, HK/F6PK FIA-ET can provide constant signal response for 3 months which is a 3-fold better stability compared to 35 days life time warranted for the FDH based amperometric sensor [33].…”

Section: Uniqueness and Advantages Of The Hk/f6pk Fia-etmentioning

confidence: 98%

“…Amperometric fructose biosensors based on fructose dehydrogenase (FDH) have been described earlier [9,[13][14][15]. The FDH based amperometric fructose biosensors reported to date though sensitive have very short operational life as well as interferences arising from ferrocene leakage [16][17][18][19][20]. The short operational stability of such sensors renders them unfit for routine analysis.…”

Section: Introductionmentioning

confidence: 98%