An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water

Lapizco‐Encinas, Blanca H.; Davalos, Rafael V.; Simmons, Blake A.; Cummings, Eric B.; Fintschenko, Yolanda

doi:10.1016/j.mimet.2005.04.027

Cited by 174 publications

(152 citation statements)

References 32 publications

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“…DEP is the movement of particles in a nonuniform electric field, and it can occur in AC or DC electric fields. DEP has been successfully applied for the manipulation of a wide range of particles: biomolecules [34][35][36][37][38], virus [39][40][41], bacteria [42][43][44][45][46][47][48][49], spores [50,51], mammalian cells [52][53][54] and parasites [55][56][57]. DEP offers the possibility of sample concentration and separation in a single step.…”

Section: Atps and Dielectrophoresismentioning

confidence: 99%

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

et al. 2008

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Aqueous Two-Phase Systems (ATPS) is a primary recovery technique that has shown great potential for the efficient extraction and purification of high value biological compounds. The main advantages of this technique include scaling up feasibility, process integration capability and biocompatibility. In this review, the efficient use of ATPS for the extraction of proteins, genetic material, low molecular weight compounds, bioparticles, nanoparticles and cells is highlighted. The important role of ATPS in process integration, i.e., extractive conversion, extractive fermentation, cell disruption integrated with product recovery, and extractive purification, is discussed. A novel approach to protein molecular characterization combining ATPS and 2-dimension electrophoresis (2-DE) is introduced as a first step in the process development. Novel approaches for downstream processing using ATPS and dielectrophoresis are presented. Finally, trends concerning the application of ATPS strategies to address the future challenges of bioseparation are discussed.

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Section: Atps and Dielectrophoresismentioning

confidence: 99%

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

et al. 2008

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“…21 Dielectrophoresis (DEP) is a powerful technique that has been used for the separation and concentration of different bioparticles including bacteria, DNA, and infected cells from blood. 9,[22][23][24][25][26][27][28] A significant challenge when using DEP for particle trapping is the need for low electrically conductive media, since most, if not all, of the relevant biological samples such as blood and urine feature high electrical conductivity, re-suspension of the sample in optimized DEP buffers is necessary. Another challenge in DEP has been improving throughput.…”

Section: Introductionmentioning

confidence: 99%

Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis

et al. 2016

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Here, we report on an enrichment protocol using carbon electrode dielectrophoresis to isolate and purify a targeted cell population from sample volumes up to 4 ml. We aim at trapping, washing, and recovering an enriched cell fraction that will facilitate downstream analysis. We used an increasingly diluted sample of yeast, 10 6 -10 2 cells/ml, to demonstrate the isolation and enrichment of few cells at increasing flow rates. A maximum average enrichment of 154.2 6 23.7 times was achieved when the sample flow rate was 10 ll/min and yeast cells were suspended in low electrically conductive media that maximizes dielectrophoresis trapping. A COMSOL Multiphysics model allowed for the comparison between experimental and simulation results. Discussion is conducted on the discrepancies between such results and how the model can be further improved. Published by AIP Publishing.

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“…It has been demonstrated that iDEP systems can significantly enrich particle concentration up to three orders of magnitude. 22 Other studies have analyzed the selectivity, particle trapping capacity, and the potential to scale down constrictions for the manipulation of nanoparticles. [23][24][25] However, there is a need to characterize the limits of iDEP systems for the isolation and enrichment of low abundant particles in complex samples.…”

Section: Introductionmentioning

confidence: 99%

Isolation and enrichment of low abundant particles with insulator-based dielectrophoresis

et al. 2015

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Isolation and enrichment of low-abundant particles are essential steps in many bio-analytical and clinical applications. In this work, the capability of an insulator-based dielectrophoresis (iDEP) device for the detection and stable capture of low abundant polystyrene particles and yeast cells was evaluated. Binary and tertiary mixtures of particles and cells were tested, where the low-abundant particles had concentration ratios on the order of 1:10 000 000 compared to the other particles present in the mixture. The results demonstrated successful and stable capture and enrichment of rare particles and cells (trapping efficiencies over 99%), where particles remained trapped in a stable manner for up to 4 min. A device with four reservoirs was employed for the separation and enrichment of rare particles, where the particles of interest were first selectively concentrated and then effectively directed to a side port for future collection and analysis. The present study demonstrates that simple iDEP devices have appropriate screening capacity and can be used for handling samples containing rare particles; achieving both enrichment and isolation of low-abundant particles and cells. V C 2015 AIP Publishing LLC. [http://dx

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An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water

Cited by 174 publications

References 32 publications

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

Extraction and Purification of Bioproducts and Nanoparticles using Aqueous Two‐Phase Systems Strategies

Enrichment of diluted cell populations from large sample volumes using 3D carbon-electrode dielectrophoresis

Isolation and enrichment of low abundant particles with insulator-based dielectrophoresis

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