An instrument to determine the magnetophoretic mobility of labeled, biological cells and paramagnetic particles

Chalmers, Jeffrey J.; Zhao, Yunhui; Nakamura, Masayuki; Melnik, Kristie; Lasky, Larry C.; Moore, Lee R.; Zborowski, Maciej

doi:10.1016/s0304-8853(98)00557-5

Cited by 76 publications

(96 citation statements)

References 7 publications

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“…The horizontal cell displacement induced by the magnetic field was analyzed to produce a cell velocity distribution. The velocities were divided by the magnitude of the energy density gradient to produce a histogram of the magnetophoretic mobility in units of millimeter cubed per tesla-ampere-second (mm 3 /T-A-s) (18)(19)(20). For the magnetic field used in these experiments, the magnetically induced cell velocity of 50 mm/s corresponded to its magnetophoretic mobility of 3.4 3 10 24 mm 3 /T-A-s.…”

Section: Ctv Analysismentioning

confidence: 99%

“…The extent of cell motion in the magnetic field, referred to as magnetophoretic mobility, is related to magnetic labeling intensity and is an important parameter governing flow mode sorting. Cell Tracking Velocimetry (CTV) has been developed from particle tracking velocimetry to evaluate the movement of a labeled cell population within a magnetic field (17)(18)(19)(20). This technique involves capturing images of cells moving through a medium of known viscosity and mag-netic susceptibility in a well-defined magnetic energy density gradient, which is then analyzed to produce a histogram of the magnetophoretic mobility.…”

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confidence: 99%

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Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies

et al. 2005

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Background: Continuous flow immunomagnetic separation is an attractive alternative to current batch mode immunomagnetic separation methods because it is capable of high sorting speeds at mild cell conditions, and grants the operator better control of separation process. The control of the separation is dependent on knowledge of the amount of magnetic label attached to the cell (magnetic labeling intensity), however. Determination of the magnetic labeling is accomplished by measuring cell magnetophoretic mobility using a newly developed technique of Cell Tracking Velocimetry (CTV). Methods: Flow cytometry was used to define the antibody binding characteristics of a fluorescently tagged primary antibody. Subsequently, CTV was used to measure antibody‐binding characteristics of a magnetically tagged secondary antibody. Results: The results of this study show that CTV is capable of providing valuable information concerning the cell labeling by magnetically tagged antibodies. It was demonstrated that the magnetically conjugated antibody binding curve exhibits the same exponential increase to saturation characteristics as that seen with the fluorescently tagged antibody. Further, it was shown that the intensity of the secondary magnetic labeling is directly proportional to the intensity of the primary fluorescent label. Conclusions: CTV is an accurate tool for evaluation of magnetically conjugated antibodies. The ability to determine the intensity of magnetic labeling is necessary for the development of continuous flow immunomagnetic separations based on cell magnetophoresis. © 2005 Wiley‐Liss, Inc.

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Section: Ctv Analysismentioning

confidence: 99%

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confidence: 99%

Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies

et al. 2005

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“…They have been applied in a variety of medical applications such as magnetic cell separation, drug delivery, RF heating of tumours and MRI contrast agents (Pankhurst et al 2003). Mobility measurements of cells and antibody binding have been measured with magnetically labelled cells (Chalmers et al 1999a) as have a variety of cell properties (Chalmers et al 1999b).…”

Section: Nanoparticle Propertiesmentioning

confidence: 99%

A biomagnetic system forin vivocancer imaging

Flynn

Bryant

2005

Phys. Med. Biol.

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An array of highly sensitive biomagnetic sensors of the superconducting quantum interference detector (SQUID) type can identify disease in vivo by detecting and imaging microscopic amounts of nanoparticles. We describe in detail procedures and parameters necessary for implementation of in vivo detection through the use of antibody-labelled magnetic nanoparticles as well as methods of determining magnetic nanoparticle properties. We discuss the weak field magnetic sensor SQUID system, the method of generating the magnetic polarization pulse to align the magnetic moments of the nanoparticles, and the measurement techniques to measure their magnetic remanence fields following this pulsed field. We compare these results to theoretical calculations and predict optimal properties of nanoparticles for in vivo detection.

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“…Particle Tracking Velocimetry (PTV) and magnetophoresis of immunomagnetically labeled cells was combined by Zborowski et al [35][36][37][38][39][40][41] They measured the magnetophoretic velocity of an individual labeled cell under an inhomogeneous magnetic field, and then the magnetophoretic mobility was determined. In their theoretical treatment, the magnetophoretic mobility depended on the number of magnetic labels.…”

Section: ·3 Microparticle Analysis By Magnetophoretic Velocimetrymentioning

confidence: 99%

Migration Analysis of Micro-Particles in Liquids Using Microscopically Designed External Fields

et al. 2004

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The recent development of new migration methods of micro-particles in liquids using various external fields is reviewed. The combination of a laser scattering force and a photothermal effect produced photothermal-conversion laserphotophoresis. A dielectric field generated in a planer or a capillary quadrupole electrode realized dielectrophoresis. Using a micrometer-scaled magnetic field gradient, the "Magnetophoretic velocimetry" of micro-particles was invented. Furthermore, the Lorentz force generated by combining an electric field and a magnetic field was utilized for electromagnetophoresis. These new methods were overlooked and the advantages in analytical use were discussed.

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An instrument to determine the magnetophoretic mobility of labeled, biological cells and paramagnetic particles

Cited by 76 publications

References 7 publications

Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies

Cell tracking velocimetry as a tool for defining saturation binding of magnetically conjugated antibodies

A biomagnetic system forin vivocancer imaging

Migration Analysis of Micro-Particles in Liquids Using Microscopically Designed External Fields

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