An insight into the sialome of the adult female mosquito Aedes albopictus

Arcà, Bruno; Lombardo, Fabrizio; Francischetti, Ivo M.B.; Pham, Van My; Mestres-Simon, Montserrat; Andersen, John F.; Ribeiro, José M. C.

doi:10.1016/j.ibmb.2006.10.007

Cited by 120 publications

(151 citation statements)

References 64 publications

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“…The remaining transcripts code for other H functions, as indicated in Table 1, and have <2 ESTs per contig. This distribution is typical of previous mosquito sialotranscriptomes (Valenzuela et al 2003;Ribeiro et al 2004Ribeiro et al ,2007Arca et al 2007;Calvo et al 2007a). …”

Section: Characteristics Of the Assembled Salivary Est Setsupporting

confidence: 60%

“…These five sequences are consistent with the existence of two genes providing for alleles and splice variants. The multigene pattern has been observed previously for Aedes and Culex mosquitoes (Ribeiro et al 2004Arca et al 2007), whereas anophelines to date appear to have only one gene (Valenzuela et al 2003, Arca et al 2005, Calvo et al 2009b. One of the Aedes and one of the Anopheles proteins have been characterized as inhibitors of collagen-induced platelet aggregation by their strong interaction with collagen (Calvo et al 2007b, Yoshida et al 2008).…”

Section: Proteins Common To Sialomes Of Blood-sucking Nematocera or Cmentioning

confidence: 75%

“…quinquefasciatus (Ribeiro et al 2004) The WPCWW Family-Previous sialotranscriptomes identified several proteins by the generic name of W or tryptophane rich. OT-84, OT-86, OT-87, and OT-89 code for slightly different (possibly alleles) members of the W-rich family, previously described in Aedes and Culex (but not anopheline) sialotranscriptomes (Ribeiro et al 2004Arca et al 2007). …”

Section: Proteins Found To Date Only In Culicines (Not Found In Anophmentioning

confidence: 99%

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The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito,Ochlerotatus triseriatus

et al. 2010

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Saliva of blood-sucking arthropods contains a complex mixture of peptides that affect their host's hemostasis, inflammation, and immunity. These activities can also modify the site of pathogen delivery and increase disease transmission. Saliva also induces hosts to mount an antisaliva immune response that can lead to skin allergies or even anaphylaxis. Accordingly, knowledge of the salivary repertoire, or sialome, of a mosquito is useful to provide a knowledge platform to mine for novel pharmacological activities, to develop novel vaccine targets for vector-borne diseases, and to develop epidemiological markers of vector exposure and candidate desensitization vaccines. The mosquito Ochlerotatus triseriatus is a vector of La Crosse virus and produces allergy in humans. In this work, a total of 1,575 clones randomly selected from an adult female O. triseriatus salivary gland cDNA library was sequenced and used to assemble a database that yielded 731 clusters of related sequences, 560 of which were singletons. Primer extension experiments were performed in selected clones to further extend sequence coverage, allowing for the identification of 159 protein sequences, 66 of which code for putative secreted proteins. Supplemental spreadsheets containing these data are available at http://exon.niaid.nih.gov/transcriptome/Ochlerotatus_triseriatus/S1/Ot-S1.xls and http://exon.niaid.nih.gov/transcriptome/Ochlerotatus_triseriatus/S2/Ot-S2.xls.

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Section: Characteristics Of the Assembled Salivary Est Setsupporting

confidence: 60%

Section: Proteins Common To Sialomes Of Blood-sucking Nematocera or Cmentioning

confidence: 75%

Section: Proteins Found To Date Only In Culicines (Not Found In Anophmentioning

confidence: 99%

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The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito,Ochlerotatus triseriatus

et al. 2010

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“…In the past decade, transcriptomic and proteomic studies have greatly increased our knowledge of the sialomes of a number of arthropod vectors (1,2), with transcriptomic studies in Anopheles gambiae, Aedes aegypti, and Culex pipiens specifically increasing our understanding of the mixture of proteins found in mosquito salivary glands (15)(16)(17). During a transcriptomic analysis of female A. gambiae salivary glands, Arcà et al (16) identified a contiguous series of large genes of unknown function (Vector base IDs: ENSANGP00000027299, ENSANGP00000027791, and ENSANGP00000029569; later named SGS 2 by Korochkina et al (18)) that had not been predicted during earlier genome scans and that appear to have been horizontally transferred into the mosquito genome from a bacterial source whose only known living relatives are Wolbachia proteobacteria (19).…”

mentioning

confidence: 99%

Members of the Salivary Gland Surface Protein (SGS) Family Are Major Immunogenic Components of Mosquito Saliva

King

Vernick

Hillyer

2011

Journal of Biological Chemistry

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Background: During feeding, mosquito saliva modulates the vertebrate host's hemostasis and inflammation response. Results: Anopheles gambiae Sgs4 and Sgs5 are major immunogenic proteins in mosquito saliva. Conclusion: Sgs4 and Sgs5 appear to play an essential role in blood feeding. Significance: Sgs4 and Sgs5 could serve as markers of human exposure to mosquito bites and in the development of disease control strategies.

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“…EST sequences were clustered together and a series of bioinformatics analyses was then applied to each consensus sequence. The results of these analyses were collated into a spreadsheet that can be queried or browsed to identify the function of each transcript cluster consensus Santos et al, 2004;Arca et al, 2007).…”

Section: Genome Vs Estsmentioning

confidence: 99%

Genomic resources for invertebrate vectors of human pathogens, and the role of VectorBase

Mégy

Hammond

Lawson

et al. 2009

Infection, Genetics and Evolution

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High-throughput genome sequencing techniques have now reached vector biology with an emphasis on those species that are vectors of human pathogens. The first mosquito to be sequenced was Anopheles gambiae, the vector for Plasmodium parasites that cause malaria. Further mosquitoes have followed: Aedes aegypti (Yellow fever and Dengue fever vector) and Culex pipiens (lymphatic filariasis and West Nile fever). Species that are currently in sequencing include the body louse Pediculus humanus (Typhus vector), the triatomine Rhodnius prolixus (Chagas disease vector) and the tick Ixodes scapularis (Lyme disease vector). The motivations for sequencing vector genomes are to further understand vector biology, with an eye on developing new control strategies (for example novel chemical attractants or repellents) or understanding the limitations of current strategies (for example the mechanism of insecticide resistance); to analyse the mechanisms driving their evolution; and to perform an exhaustive analysis of the gene repertory. The proliferation of genomic data creates the need for efficient and accessible storage. We present VectorBase, a genomic resource centre that is both involved in the annotation of vector genomes and act as a portal for access to the genomic information (http://www.vectorbase.org).

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An insight into the sialome of the adult female mosquito Aedes albopictus

Cited by 120 publications

References 64 publications

The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito,Ochlerotatus triseriatus

The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito,Ochlerotatus triseriatus

Members of the Salivary Gland Surface Protein (SGS) Family Are Major Immunogenic Components of Mosquito Saliva

Genomic resources for invertebrate vectors of human pathogens, and the role of VectorBase

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