An insight into the sialome of Hyalomma excavatum

Ribeiro, José M. C.; Slovák, Mirko; Francischetti, Ivo M.B.

doi:10.1016/j.ttbdis.2016.08.011

Cited by 39 publications

(44 citation statements)

References 39 publications

(46 reference statements)

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“…Thus, knowledge about the vital molecular constituents of ticks, such as genes and/or proteins, have become the target of several studies (CASTRO et al, 2016;CHMELAŘ et al, 2016;DÍAZ-MARTÍN et al, 2015;HEEKIN et al, 2013;KOTSYFAKIS et al, 2015;PERNER et al, 2016;RIBEIRO et al, 2017;XU et al, 2016). These studies have sought to ascertain and understand the roles of the molecules involved in the tick-host or tick-pathogen relationship.…”

Section: Introductionmentioning

confidence: 99%

“…These studies have sought to ascertain and understand the roles of the molecules involved in the tick-host or tick-pathogen relationship. In addition, there is great interest in discovering new molecules with potential for use within biotechnology and pharmacology, which might help in developing new control methods (CHMELAŘ et al, 2016;DÍAZ-MARTÍN et al, 2015;RIBEIRO et al, 2017). The evolutionary context of bloodsucking organisms is another prominent approach that has been studied, through making inferences about the evolution of genes and proteins, thereby helping to improve knowledge about the hematophagic behavior of the arthropods (MANS et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…DNA sequencing technologies, together with bioinformatics tools, allow a broad view of the probable protein constituents of a tissue, by isolating the messenger RNA repertoire and sequencing of the cDNA (complementary DNA), leading to an increasing knowledge of the putative molecules expressed by a tissue, such as, salivary gland and midgut, from different species (CASTRO et al, 2016;CHMELAŘ et al, 2016;KOTSYFAKIS et al, 2015;PERNER et al, 2016;RIBEIRO et al 2017;SCHWARZ et al, 2014;VALENZUELA, 2004;XU et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums and is also harmful to humans. Knowledge of the transcripts present in the tick gut helps in understanding the role of vital molecules in the digestion process and parasite-host relationship, while also providing information about the evolution of arthropod hematophagy. Thus, the present study aimed to know and ascertain the main molecules expressed in the gut of argasid after their blood meal, through analysis on the gut transcriptome of engorged females of O. mimon using 454-based RNA sequencing. The gut transcriptome analysis reveals several transcripts associated with hemoglobin digestion, such as serine, cysteine, aspartic proteases and metalloenzymes. The phylogenetic analysis on the peptidases confirmed that most of them are clustered with other tick genes. We recorded the presence a cathepsin O peptidase-coding transcript in ticks. The topology of the phylogenetic inferences, based on transcripts of inferred families of homologues, was similar to that of previous reports based on mitochondrial genome and nuclear rRNA sequences. We deposited 2,213 sequence of O. mimon to the public databases. Our findings may help towards better understanding of important argasid metabolic processes, such as digestion, nutrition and immunity.Keywords: Argasidae, transcriptome, gut, phylogeny, Ornithodoros mimon. ResumoOrnithodoros mimon é um carrapato argasídeo parasita de morcegos, aves e marsupiais, além de ser bastante agressivo aos humanos. O conhecimento dos transcritos presentes no intestino dos carrapatos auxilia no entendimento do papel de moléculas vitais no processo de digestão e na relação parasito-hospedeiro, além de fornecer também informações sobre a evolução dos artrópodes hematófagos. Desta maneira, o presente estudo teve como objetivo conhecer e identificar as principais moléculas expressas no intestino de uma espécie de carrapato argasídeo após o repasto sanguíneo, através de uma análise transcritômica descritiva do intestino de fêmeas ingurgitadas de O. mimon, utilizando um sequenciamento de RNA de nova geração da plataforma 454. Além de inferir a relação filogenética de carrapatos através de um conjunto de dados transcritômicos. O transcriptoma do intestino revelou diversos transcritos associados com a digestão da hemoglobina, como proteinases das classes serino, cisteína, aspártica e metalo. Registramos a presença de um transcrito de uma cisteína peptidase do tipo catepsina O em carrapatos. A inferência filogenética baseada em conjunto de dados transcritos homólogos tem uma resolução topológica similar a de outros conjuntos de dados moleculares. Foram depositados no banco de dados gênico público 2213 transcritos de O. mimon. Os achados obtidos no presente estudo podem contribuir para compreensão dos importantes processos, como digestão, nutrição e imunidade dos carrapatos da família Argasidae, além de fornecer informações sobre a filogenia da ordem Ixodida.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…Custom bioinformatic analysis were describe elsewhere (Karim, Singh, & Ribeiro, ; Ribeiro, Slovak, & Francischetti, ) with modifications. Low‐quality reads were trimmed from Fastq files (less than 20) and contaminating adapter primer sequences were removed.…”

Section: Methodsmentioning

confidence: 99%

The relapsing fever spirochete Borrelia turicatae persists in the highly oxidative environment of its soft‐bodied tick vector

Bourret

Boyle

Zalud

et al. 2019

Cellular Microbiology

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The relapsing fever spirochete Borrelia turicatae possesses a complex life cycle in its soft‐bodied tick vector, Ornithodoros turicata . Spirochetes enter the tick midgut during a blood meal, and, during the following weeks, spirochetes disseminate throughout O. turicata . A population persists in the salivary glands allowing for rapid transmission to the mammalian hosts during tick feeding. Little is known about the physiological environment within the salivary glands acini in which B. turicatae persists. In this study, we examined the salivary gland transcriptome of O. turicata ticks and detected the expression of 57 genes involved in oxidant metabolism or antioxidant defences. We confirmed the expression of five of the most highly expressed genes, including glutathione peroxidase ( gpx ), thioredoxin peroxidase ( tpx ), manganese superoxide dismutase ( sod‐1 ), copper‐zinc superoxide dismutase ( sod‐2 ), and catalase ( cat ) by reverse‐transcriptase droplet digital polymerase chain reaction (RT‐ddPCR). We also found distinct differences in the expression of these genes when comparing the salivary glands and midguts of unfed O. turicata ticks. Our results indicate that the salivary glands of unfed O. turicata nymphs are highly oxidative environments where reactive oxygen species (ROS) predominate, whereas midgut tissues comprise a primarily nitrosative environment where nitric oxide synthase is highly expressed. Additionally, B. turicatae was found to be hyperresistant to ROS compared with the Lyme disease spirochete Borrelia burgdorferi , suggesting it is uniquely adapted to the highly oxidative environment of O. turicata salivary gland acini.

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“…They found significant change in the banding pattern of salivary gland proteins of ticks incubated at various temperatures. In a study conducted by Ribeiro et al (2017), it has been observed that mucins, glycine-rich proteins, anticoagulants of the madanin family, metalloproteases and lipocalins are the proteins among the most secreted by H. excavatum.…”

Section: Significance Of Salivary Glands Of Hyalommamentioning

confidence: 99%

An insight into the ecobiology, vector significance and control of Hyalomma ticks (Acari: Ixodidae): A review

Sajid

Kausar²,

Iqbal

et al. 2018

Acta Tropica

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Ticks (Acari:Ixodoidea) are important ectoparasites infesting livestock and human populations around the globe. Ticks can cause damage directly by affecting the site of infestation, or indirectly as vectors of a wide range of protozoa, bacteria and viruses which ultimately lead to lowered productivity of livestock populations. Hyalomma is a genus of hard ticks, having more than 30 species well-adapted to hot, humid and cold climates. Habitat diversity, vector ability, and emerging problem of acaricidal resistance in enzootic regions typify this genus in various countries around the world. This paper reviews the epidemiology, associated risk factors (temperature, climate, age, sex, breed etc.), vector role, vector-pathogen association, and reported control strategies of genus Hyalomma. The various proteins in saliva of Hyalomma secreted into the blood stream of host and the prolonged attachment are responsible for the successful engorgement of female ticks in spite of host immune defense system. The various immunological approaches that have been tried by researchers in order to cause tick rejection are also discussed. In addition, the novel biological control approaches involving the use of entomo-pathogenic nematodes and Bacillus thuringiensis (B. thuringiensis) serovar thuringiensis H14; an endotoxin, for their acaricidal effect on different species and life cycle stages of Hyalomma are also presented.

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An insight into the sialome of Hyalomma excavatum

Cited by 39 publications

References 39 publications

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

The relapsing fever spirochete Borrelia turicatae persists in the highly oxidative environment of its soft‐bodied tick vector

An insight into the ecobiology, vector significance and control of Hyalomma ticks (Acari: Ixodidae): A review

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