An insight into the production strategies and applications of the ligninolytic enzyme laccase from bacteria and fungi

Debnath, Rinku; Saha, Tanima

doi:10.1016/j.bcab.2020.101645

Cited by 59 publications

(28 citation statements)

References 237 publications

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“…Solid-State Fermentation (SSF). SSF is not the main method in industrial-scale enzyme production; however, through SSF, ligninolytic enzyme production is higher and cost-effective rather than submerged fermentation (SmF) [64]. Lignocellulolytic enzyme production by different microbes is often enhanced in SSF cultivation for the reason it contains a growth substrate with a significant content of valuable nutrients as free mono-and disaccharides and organic acids [65].…”

Section: Molecular Identification Of Microbial Strains the Cultural Characteristics And Morphological Features Resultsmentioning

confidence: 99%

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Zhang

Shah

Mohamed

et al. 2021

BioMed Research International

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Lignocellulosic waste is the most abundant biorenewable biomass on earth, and its hydrolysis releases highly valued reducing sugars. However, the presence of lignin in the biopolymeric structure makes it highly resistant to solubilization thereby hindering the hydrolysis of cellulose and hemicellulose. Microorganisms are known for their potential complex enzymes that play a dominant role in lignocellulose conversion. Therefore, the current study was designed to isolate and screen potential microorganisms for their selective delignification ability for the pretreatment of lignocellulosic biomass. An extensive isolation and screening procedure yielded 36 desired isolates (22 bacteria, 7 basidiomycete fungi, and 7 filamentous fungi). Submerged cultivation of these desired microorganisms revealed 4 bacteria and 10 fungi with potent lignocellulolytic enzyme activities. The potent isolates were identified as Pleurotus, Trichoderma, Talaromyces, Bacillus, and Chryseobacterium spp. confirmed by morphological and molecular identification. The efficiency of these strains was determined through enzyme activities, and the degraded substrates were analyzed through scanning electron microscopy (SEM) and X-ray diffraction (XRD). Among all isolated microbes, Pleurotus spp. were found to have high laccase activity. The cellulose-decomposing and selective delignification strains were subjected to solid-state fermentation (SSF). SSF of field waste corn stalks as a single-carbon source provides Pleurotus spp. better condition for the secretion of ligninolytic enzymes. These isolated ligninolytic enzymes producing microorganisms may be used for the effective pretreatment of lignocellulosic agricultural wastes for the production of high value-added natural products by fermentation.

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Section: Molecular Identification Of Microbial Strains the Cultural Characteristics And Morphological Features Resultsmentioning

confidence: 99%

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Zhang

Shah

Mohamed

et al. 2021

BioMed Research International

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“…MnP-producing yeasts have been shown to be highly effective at converting textile azo dye wastewater into innocuous end products [ 50 ]. Ligninolytic yeasts produce a variety of peroxidases, which are used in lignin degradation, dye decolorization, and phenolics removal [ 51 ]. Biomass digestion by termites, on the other hand, is far more efficient, typically achieving over 95% within a day [ 37 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Wood-feeding termite gut symbionts as an obscure yet promising source of novel manganese peroxidase-producing oleaginous yeasts intended for azo dye decolorization and biodiesel production

Al-Tohamy

Sun

Khalil

et al. 2021

Biotechnol Biofuels

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Background The ability of oxidative enzyme-producing micro-organisms to efficiently valorize organic pollutants is critical in this context. Yeasts are promising enzyme producers with potential applications in waste management, while lipid accumulation offers significant bioenergy production opportunities. The aim of this study was to explore manganese peroxidase-producing oleaginous yeasts inhabiting the guts of wood-feeding termites for azo dye decolorization, tolerating lignocellulose degradation inhibitors, and biodiesel production. Results Out of 38 yeast isolates screened from wood-feeding termite gut symbionts, nine isolates exhibited high levels of extracellular manganese peroxidase (MnP) activity ranged between 23 and 27 U/mL after 5 days of incubation in an optimal substrate. Of these MnP-producing yeasts, four strains had lipid accumulation greater than 20% (oleaginous nature), with Meyerozyma caribbica SSA1654 having the highest lipid content (47.25%, w/w). In terms of tolerance to lignocellulose degradation inhibitors, the four MnP-producing oleaginous yeast strains could grow in the presence of furfural, 5-hydroxymethyl furfural, acetic acid, vanillin, and formic acid in the tested range. M. caribbica SSA1654 showed the highest tolerance to furfural (1.0 g/L), 5-hydroxymethyl furfural (2.5 g/L) and vanillin (2.0 g/L). Furthermore, M. caribbica SSA1654 could grow in the presence of 2.5 g/L acetic acid but grew moderately. Furfural and formic acid had a significant inhibitory effect on lipid accumulation by M. caribbica SSA1654, compared to the other lignocellulose degradation inhibitors tested. On the other hand, a new MnP-producing oleaginous yeast consortium designated as NYC-1 was constructed. This consortium demonstrated effective decolorization of all individual azo dyes tested within 24 h, up to a dye concentration of 250 mg/L. The NYC-1 consortium's decolorization performance against Acid Orange 7 (AO7) was investigated under the influence of several parameters, such as temperature, pH, salt concentration, and co-substrates (e.g., carbon, nitrogen, or agricultural wastes). The main physicochemical properties of biodiesel produced by AO7-degraded NYC-1 consortium were estimated and the results were compared to those obtained from international standards. Conclusion The findings of this study open up a new avenue for using peroxidase-producing oleaginous yeasts inhabiting wood-feeding termite gut symbionts, which hold great promise for the remediation of recalcitrant azo dye wastewater and lignocellulosic biomass for biofuel production. Graphical Abstract

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“…In recent times, laccases have been continuously used in many domains, such as the food industry (wine stabilization, fruit juice clarification, baking, and sugar beet pectin production); the textile industry (fiber biobleaching, denim washing); polymer synthesis and green nanoparticle synthesis; the paper and pulp industry (delignification of pulp, and green nanoparticle synthesis; the paper and pulp industry (delignification of pulp, deinking of paper); biosensors; the decomposition of lignocellulosic material used as substrate in various processes; etc. [3].…”

Section: Importance Of Laccases For Environment and Industrymentioning

confidence: 99%

Laccases—Versatile Enzymes Used to Reduce Environmental Pollution

et al. 2022

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The accumulation of waste and toxic compounds has become increasingly harmful to the environment and human health. In this context, the use of laccases has become a focus of interest, due to the properties of these versatile enzymes: low substrate specificity, and water formation as a non-toxic end product. Thus, we begin our study with a general overview of the importance of laccase for the environment and industry, starting with the sources of laccases (plant, bacterial and fungal laccases), the structure and mechanism of laccases, microbial biosynthesis, and the immobilization of laccases. Then, we continue with an overview of agro-waste treatment by laccases wherein we observe the importance of laccases for the biodisponibilization of substrates and the biodegradation of agro-industrial byproducts; we then show some aspects regarding the degradation of xenobiotic compounds, dyes, and pharmaceutical products. The objective of this research is to emphasize and fully investigate the effects of laccase action on the decomposition of lignocellulosic materials and on the removal of harmful compounds from soil and water, in order to provide a sustainable solution to reducing environmental pollution.

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An insight into the production strategies and applications of the ligninolytic enzyme laccase from bacteria and fungi

Cited by 59 publications

References 237 publications

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Wood-feeding termite gut symbionts as an obscure yet promising source of novel manganese peroxidase-producing oleaginous yeasts intended for azo dye decolorization and biodiesel production

Laccases—Versatile Enzymes Used to Reduce Environmental Pollution

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