An insight into synthesis and antitumor activity of citrate and gallate stabilizing gold nanospheres

Abstract

Both gallic and citrate are well-established antioxidants that show promise as new selective anti-cancer drugs. Gold nanoparticles (AuNPs) as well can be developed as flexible and nontoxic nano-carriers for anti-cancer drugs. This article evaluating the efficiency and biocompatibility of gallic acid and citrate capping gold nanoparticles to be used as anti-cancer drug. The biosafety and therapeutic efficiency of prepared nano-formulations were tested on Hela and normal BHK cell line. Gold nanospheres coated with citrate and gallate were synthesized via wet chemical reduction method. The prepared nano-formulations, citrate and gallate coated gold nanospheres (Cit-AuNPs and Ga-AuNPs), were characterized with respect to their morphology, FTIR spectra, and physical properties. In addition, to assess their cytotoxicity, cell cycle arrest and flow cytometry to measure biological response were performed. Cit-Au NPs and Ga-Au NPs were shown to significantly reduce the viability of Hela cancer cells. Both G0/G cell cycle arrest and comet assay results showed that genotoxic effect was induced in Hela cells by Cit-Au NPs and Ga-Au NPs. The results of this study showed that Cit-Au NPs and Ga-AuNPs inhibit the growth of metastatic cervical cancer cells, which could have therapeutic implications.

Poly(ethylene glycol) Patterned Surfaces Functionalized with Gallic Acid@Au Nanoparticles: Investigation of Antibacterial Activity for Biomedical Applications

“…It was last characterized by UV visible, STEM, DLS and Zeta Potential. (Fathy et al 2023) Immobilization of GA@Au NPs Au nanoparticles were dropped onto the grafted polymer pattern surfaces and incubated for 1 hour at room conditions. In order to create a humidity balance in the environment, the immobilization medium was moistened in accordance with the pattern surface area.…”

Tau- and α-synuclein-targeted gold nanoparticles: applications, opportunities, and future outlooks in the diagnosis and therapy of neurodegenerative diseases

“…Gallic acid is another successful case used to synthesize GNP. This phytochemical is present in tea leaves, fruits, and nuts and has been used to synthesize GNS with a potential application as an antitumoral or anti-aging agent [81][82][83].…”