An insight into polyscopoletin electrosynthesis by a quality-by-design approach

Abstract

Scopoletin (SP) as a functional monomer for electropolymerization has recently been investigated in the context of molecularly imprinted polymers for biosensing applications. Herein we describe an in-depth analysis of the mechanisms involved in the electropolymerization of SP toward the optimization of the experimental conditions for applications in sensor studies. PolySP films have been in situ synthesized on a standard glassy carbon electrodes by varying three independent experimental parameters, and the output of the analysis has been evaluated in terms of the resulting electroactive area and surface coverage. A quality-by-design approach including design-of-experiments principles and response surface methodology produced unbiased observations on the most relevant parameters to be controlled during the electropolymerization of SP. By combining the output of electroactive area and surface overage, we highlighted a strong dependence on the monomer concentration and scan rate. Thus, an appropriate selection of these two parameters should be sought to have an optimal electropolymerization process, leading to uniform films and homogeneous surface behavior. This study shows that the application of multi-factorial analysis in a comprehensive design of experiments allows the systematic study of polymer electrosynthesis. Therefore, this research is expected to guide further efforts in the electropolymerization of several functional monomers.

Molecularly Imprinted Polymer-Based Electrochemical Sensing in Protein Detection

“…The functional monomer conforms to the target protein's shape and chemical properties, creating precise binding sites. The cross-linking agent reinforces the polymer structure [18,19] . The resulting polymer matrix accurately replicates the target protein's structure and behaviour.…”

Nanomaterial-Based Sensors for Coumarin Detection

“…Structurally, scopoletin has two aromatic rings supported by a hydroxyl group substituent and oxo and methoxy groups . Recently, further research has been conducted on scopoletin as a functional monomer in electropolymerization in molecularly imprinted polymers intended for sensor use . Scopoletin is used as a monomer in the synthesis of sensitive polymers due to its advantages, such as easy polymerization, low cost, water solubility, and the ability to use aqueous solutions rather than toxic solvents. , Esculetin, also known as 6,7-dihydroxycoumarin, is one of the simplest coumarins.…”

Effect of Recombinant Antibodies and MIP Nanoparticles on the Electrical Behavior of Impedimetric Biorecognition Surfaces for SARS-CoV-2 Spike Glycoprotein: A Short Report

“…Owing to the vast number of recombinant antibodies for SARS-CoV-2, and their varied binding behavior to the antigens, as well as the limited stability of these molecules, some groups have proposed the use of artificial receptors, namely molecularly imprinted polymers nanoparticles (NanoMIP) in the development of reliable and robust biosensing platforms [21][22][23]. Molecular imprinting technologies are based on the topological imprinting of targets on the surface of a polymer, thereby leading to a cavity of shape and size equal to that of the target [24,25]. This very feature grants MIP-based sensors their acknowledged selectivity, while the polymer nature confers stability due to the inherent robustness of polymeric networks [26].…”