An insect TEP in a crustacean is specific for cuticular tissues and involved in intestinal defense

Wu, Chenglin; Noonin, Chadanat; Jiravanichpaisal, Pikul; Söderhäll, Irene; Söderhäll, Kenneth

doi:10.1016/j.ibmb.2011.10.006

Cited by 36 publications

(14 citation statements)

References 48 publications

(68 reference statements)

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“…4 (Adams et al, 2000;Ariki et al, 2008;Colbourne et al, 2011;Honeybee Genome Sequencing Consortium, 2006;International Aphid Genomics Consortium, 2010;Iwaki et al, 1996;Tribolium Genome Sequencing Consortium et al, 2008;Wu et al, 2012;Zhu et al, 2005). Here, + indicates the presence of any kind of evidence obtained by targeted reverse transcription polymerase chain reaction (RT-PCR), RNA-seq, or genome analysis, whereas a -symbol indicates failure of detection by the comprehensive methods such as RNA-seq or genome analysis.…”

Section: Discussionmentioning

confidence: 99%

Evolution of the complement system in protostomes revealed by de novo transcriptome analysis of six species of Arthropoda

Sekiguchi

Nonaka

2015

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 99%

Evolution of the complement system in protostomes revealed by de novo transcriptome analysis of six species of Arthropoda

Sekiguchi

Nonaka

2015

Developmental & Comparative Immunology

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“…In Drosophila melanogaster , it is reported that the expressions of TEP1 and TEP2 are JAK/STAT-dependent and upregulated in response to the bacteria challenge 21 , suggesting the involvement of TEP1 and TEP2 in the innate immunity of invertebrates. To evaluate the roles of TEP1 and TEP2 in the virus infection, the two genes were characterized in shrimp.…”

Section: Resultsmentioning

confidence: 99%

“…TEP families of proteins, which are characterized by a unique intrachain β-cysteinyl-γ-glutamyl thioester bond, are classified into two subfamilies: the alpha-2-macroglobulin (A2M) subfamily and the C3 subfamily 20 . Pl-A2M2 from Pseudomonas libanensis/gessardii may be important for the immune defense in crayfish intestine and function as a pattern recognition protein in crayfish cuticular tissues 21 .…”

mentioning

confidence: 99%

A white spot syndrome virus microRNA promotes the virus infection by targeting the host STAT

Ren¹,

Huang²,

He³

et al. 2015

Sci Rep

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JAK/STAT pathway plays an important role in invertebrates during virus infection. However the microRNA (miRNA)-mediated regulation of JAK/STAT is not intensively investigated. Viral miRNAs, encoded by virus genome, have emerged as important regulators in the virus-host interactions. In this study, a WSSV (white spot syndrome virus)-encoded miRNA (WSSV-miR-22) was characterized in shrimp during virus infection. The results showed that the viral miRNA could promote WSSV infection in shrimp by targeting the host STAT gene. When the expression of JAK or STAT was knocked down by sequence-specific siRNA, the WSSV copies in shrimp were significantly increased, indicating that the JAK/STAT played positive roles in the antiviral immunity of shrimp. The further findings revealed that TEP1 and TEP2 were the effectors of JAK-STAT signaling pathway. The silencing of TEP1 or TEP2 led to an increase of WSSV copies in shrimp, showing TEP1 and TEP2 were involved in the shrimp immune response against virus infection. Therefore our study presented a novel viral miRNA-mediated JAK/STAT-TEP1/TEP2 signaling pathway in virus infection.

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“…Thus, the injection dosage of dsRNA depends on different situations. For example, 120 μg of dsPl-TEP was injected into crayfish (20 ± 2 g) for the RNAi of Pl-TEP ( 26 ); in Penaeus monodon , the dosage of PmTBC1D20 dsRNA was 10 μg/g shrimp used in the RNAi assay of PmTBC1D20 ( 27 ). Here in our study, we injected 30 μg dsRNA/shrimp to get the optimal RNAi efficiency.…”

Section: Discussionmentioning

confidence: 99%

A Small GTPase, RhoA, Inhibits Bacterial Infection Through Integrin Mediated Phagocytosis in Invertebrates

Diao

Niu

et al. 2018

Front. Immunol.

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The Ras GTPase superfamily, including more than 100 members, plays a vital role in a number of cellular processes, such as cytoskeleton recombination, gene expression, and signaling pathway regulation. Some members of the superfamily participate in innate immunity in animals. However, there have been few studies of RhoA on this aspect. In the present study, we identified a RhoA GTPase in the shrimp Marsupenaeus japonicus and named it MjRhoA. Expression of MjRhoA was significantly upregulated in hemocytes and heart of shrimp challenged with Vibrio anguillarum. Overexpression of MjRhoA in shrimp caused the total bacterial number to decrease significantly and knockdown of MjRhoA increased the bacterial number obviously, with a consequent decline in shrimp survival. These results confirmed the antibacterial function of MjRhoA in shrimp. Further study showed that rate of phagocytosis of hemocytes was decreased in MjRhoA-knockdown shrimp. Interestingly, we observed that MjRhoA was translocated onto the hemocyte membrane at 1 h post V. anguillarum challenge. The expression levels of the β-integrin-mediated phagocytosis markers ROCK2 and Arp2/3 declined significantly after knockdown of MjRhoA. These results suggested that the antibacterial function of MjRhoA was related to β-integrin-mediated phagocytosis in shrimp. Our previous study identified that a C-type lectin, hFcLec4, initiated β-integrin mediated phagocytosis after bacterial infection. Thus, knockdown of hFcLec4 and β-integrin was performed. The results showed that the translocation of MjRhoA from the cytoplasm to membrane was inhibited and the expression level of MjRhoA was decreased, suggesting that MjRhoA participated in hFcLec4-integrin mediated phagocytosis. Therefore, our study identified a new hFcLec4-integrin-RhoA dependent phagocytosis against bacterial infection in shrimp.

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An insect TEP in a crustacean is specific for cuticular tissues and involved in intestinal defense

Cited by 36 publications

References 48 publications

Evolution of the complement system in protostomes revealed by de novo transcriptome analysis of six species of Arthropoda

Evolution of the complement system in protostomes revealed by de novo transcriptome analysis of six species of Arthropoda

A white spot syndrome virus microRNA promotes the virus infection by targeting the host STAT

A Small GTPase, RhoA, Inhibits Bacterial Infection Through Integrin Mediated Phagocytosis in Invertebrates

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