An Innovative Strategy for the Effective Reduction of MDR Pathogens from the Nosocomial Environment

Caselli, Elisabetta; D’Accolti, Maria; Soffritti, Irene; Lanzoni, Luca; Bisi, Matteo; Volta, Antonella; Berloco, Filippo; Mazzacane, Sante

doi:10.1007/5584_2019_399

Cited by 16 publications

(28 citation statements)

References 59 publications

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“…In contrast, we recently reported that a sanitation approach based on the microbiomebalance principle (PCHS, Probiotic Cleaning Hygiene System), could stably and efficiently counteract pathogens compared to chemical disinfectants, through competitive exclusion mechanisms [64], decreasing pathogens 80% more than chemical disinfectants [16,22,47,65], without selecting resistant microbes but rather decreasing the existing AMR up to 99.9% [46,48,66], and also decreasing the HAI incidence (−52%) and the HAI-associated drug consumption (−65%) [46].…”

Section: Discussionmentioning

confidence: 99%

“…Aiming to obtain a long-term effective cleaning procedure, stably reducing viral contamination without impacting on environmental pollution and AMR, we tested the antiviral properties of an eco-sustainable probiotic-based sanitation system (PCHS, Probiotic Cleaning Hygiene System), that was previously shown to prevent pathogen recontamination by stably remodulating the hospital microbiome. Its action, based on the biological properties of selected probiotic Bacillus species contained in an eco-friendly detergent, stably reduced resistant pathogens (−80%) [16,22,[45][46][47][48] and associated infections (−52%) [44,46], and also had a relevant positive impact on antimicrobial consumptions (−60%) and therapy costs (−75%) [46]. Based on these observations, here we assessed the PCHS antiviral efficiency on different enveloped viruses known to be able to persist long on surfaces, namely human coronaviruses HCoV-229E and SARS-CoV-2, HSV-1, type A influenza viruses of human (human H3N2) and animal (avian H10N1 and swine H1N2) origin, and the modified Vaccinia virus Ankara (MVA), this virus being the most resistant among enveloped viruses and for this reason mandatorily included in the European standard procedures used to assess the antiviral properties of disinfectants.…”

Section: Introductionmentioning

confidence: 99%

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Potential of an Eco-Sustainable Probiotic-Cleaning Formulation in Reducing Infectivity of Enveloped Viruses

D’Accolti

Soffritti

Bonfante

et al. 2021

Viruses

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The COVID-19 pandemic has deeply influenced sanitization procedures, and high-level disinfection has been massively used to prevent SARS-CoV-2 spread, with potential negative impact on the environment and on the threat of antimicrobial resistance (AMR). Aiming to overcome these concerns, yet preserving the effectiveness of sanitization against enveloped viruses, we assessed the antiviral properties of the Probiotic Cleaning Hygiene System (PCHS), an eco-sustainable probiotic-based detergent previously proven to stably abate pathogen contamination and AMR. PCHS (diluted 1:10, 1:50 and 1:100) was tested in comparison to common disinfectants (70% ethanol and 0.5% sodium hypochlorite), in suspension and carrier tests, according with the European UNI EN 14476:2019 and UNI EN 16777:2019 standards. Human alpha- and beta-coronaviruses hCoV-229E and SARS-CoV-2, human herpesvirus type 1, human and animal influenza viruses, and vaccinia virus were included in the study. The results showed that PCHS was able to inactivate 99.99% of all tested viruses within 1–2 h of contact, both in suspension and on surface. Notably, while control disinfectants became inactive within 2 h after application, the PCHS antiviral action persisted up to 24 h post-application, suggesting that its use may effectively allow a continuous prevention of virus spread via contaminated environment, without worsening environmental pollution and AMR concern.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potential of an Eco-Sustainable Probiotic-Cleaning Formulation in Reducing Infectivity of Enveloped Viruses

D’Accolti

Soffritti

Bonfante

et al. 2021

Viruses

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“…And Bacillus spores could also be considered as a biological sanitation strategy reducing drug‐resistant pathogens in nosocomial environment (Caselli et al . 2019).…”

Section: Discussionmentioning

confidence: 99%

Anti‐staphylococcal activity of a cyclic lipopeptide, C ₁₅ ‐bacillomycin D, produced by Bacillus velezensis NST6

Nam

Alam

Kang

et al. 2020

J. Appl. Microbiol.

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Aim The aim of this study was to assess antibacterial activity of a novel Bacillus velezensis strain NST6, and further identify its active compound against pathogenic Staphylococcus strains for clinical therapeutic applications. Methods and Results In this study, a novel B. velezensis strain NST6 harbouring strong antimicrobial activity against human pathogenic bacteria was isolated from a soil sample. The solvent extract of the strain exhibited strong antibacterial activity against Gram‐positive and Gram‐negative bacteria in disc diffusion assay and measurement of minimal inhibitory concentration and bactericidal concentration, of which it showed notable efficacy to Staphylococcus species including Staphylococcus epidermidis, Staphylococcus aureus and methicillin‐resistant S. aureus. Strong antibacterial effect against pathogenic S. aureus and low toxicity of the bacterial extract were further validated in Caenorhabditis elegans model. Moreover, by antibacterial activity‐guided fractionation using RP‐HPLC and LC‐MS, we defined C15‐bacillomycin D as the anti‐staphylococcal compound produced by the strain. Conclusion The primary anti‐staphylococcal compound from B. velezensis NST6 was identified as a cyclic lipopeptide, C15‐bacillomycin D, which proved its potential to treat Staphylococcus strains in vitro and in vivo experiments with insignificant level of toxicity. Significance and Impact of the Study We provide an alternative treatment option to Staphylococcus infections by investigating the specific anti‐staphylococcal activity of C15‐bacillomycin D produced by a B. velezensis strain.

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“…Toward this direction, studies performed by us in the healthcare settings showed that probiotic-based strategies can rebalance the hospital microbiome, leading to stable reduction of pathogen contamination, of its AMR, and of the associated infections [14,[106][107][108][109][110]. Such a cleaning system (Probiotic Cleaning Hygiene System, PCHS), consisting of an ecofriendly cleanser with added spores of probiotic bacteria belonging to the Bacillus genus, can in fact competitively exclude the re-growth of pathogenic species, including bacteria and fungi [14,109].…”

Section: A New Paradigm Of the Environmental Health: Focus On Microbi...mentioning

confidence: 99%

Pathogen Control in the Built Environment: A Probiotic-Based System as a Remedy for the Spread of Antibiotic Resistance

et al. 2022

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The high and sometimes inappropriate use of disinfectants and antibiotics has led to alarming levels of Antimicrobial Resistance (AMR) and to high water and hearth pollution, which today represent major threats for public health. Furthermore, the current SARS-CoV-2 pandemic has deeply influenced our sanitization habits, imposing the massive use of chemical disinfectants potentially exacerbating both concerns. Moreover, super-sanitation can profoundly influence the environmental microbiome, potentially resulting counterproductive when trying to stably eliminate pathogens. Instead, environmentally friendly procedures based on microbiome balance principles, similar to what applied to living organisms, may be more effective, and probiotic-based eco-friendly sanitation has been consistently reported to provide stable reduction of both pathogens and AMR in treated-environments, compared to chemical disinfectants. Here, we summarize the results of the studies performed in healthcare settings, suggesting that such an approach may be applied successfully also to non-healthcare environments, including the domestic ones, based on its effectiveness, safety, and negligible environmental impact.

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An Innovative Strategy for the Effective Reduction of MDR Pathogens from the Nosocomial Environment

Cited by 16 publications

References 59 publications

Potential of an Eco-Sustainable Probiotic-Cleaning Formulation in Reducing Infectivity of Enveloped Viruses

Potential of an Eco-Sustainable Probiotic-Cleaning Formulation in Reducing Infectivity of Enveloped Viruses

Anti‐staphylococcal activity of a cyclic lipopeptide, C ₁₅ ‐bacillomycin D, produced by Bacillus velezensis NST6

Pathogen Control in the Built Environment: A Probiotic-Based System as a Remedy for the Spread of Antibiotic Resistance

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An Innovative Strategy for the Effective Reduction of MDR Pathogens from the Nosocomial Environment

Cited by 16 publications

References 59 publications

Potential of an Eco-Sustainable Probiotic-Cleaning Formulation in Reducing Infectivity of Enveloped Viruses

Potential of an Eco-Sustainable Probiotic-Cleaning Formulation in Reducing Infectivity of Enveloped Viruses

Anti‐staphylococcal activity of a cyclic lipopeptide, C 15 ‐bacillomycin D, produced by Bacillus velezensis NST6

Pathogen Control in the Built Environment: A Probiotic-Based System as a Remedy for the Spread of Antibiotic Resistance

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Product

Resources

About

Anti‐staphylococcal activity of a cyclic lipopeptide, C ₁₅ ‐bacillomycin D, produced by Bacillus velezensis NST6