An innovative stand-alone bioreactor for the highly reproducible transfer of cyclic mechanical stretch to stem cells cultured in a 3D scaffold

Govoni, Marco; Lotti, Fabrizio; Biagiotti, Luigi; Lannocca, Maurizio; Pasquinelli, Gianandrea; Valente, Sabrina; Muscari, Claudio; Bonafè, Francesca; Caldarera, Claudio Marcello; Guarnieri, Carlo; Cavalcanti, Silvio; Giordano, Emanuele

doi:10.1002/term.1578

Cited by 20 publications

(19 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alternatively, biomaterials releasing HP/IP mimetics and contemporary conveying SCs [137] could also prolong cell activity after grafting and improve the overall process of tissue regeneration. In addition, bioreactors for high-throughput cell yield providing hypoxic cell cultures chambers should be employed to give a sufficient amount of preconditioned SCs to regenerate the injured region [138,139]. Future interdisciplinary studies investigating these issues will surely contribute to upgrade these basic researches for clinical applications.…”

Section: Discussionmentioning

confidence: 99%

Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine

et al. 2013

Self Cite

View full text Add to dashboard Cite

The efficiency of regenerative medicine can be ameliorated by improving the biological performances of stem cells before their transplantation. Several ex-vivo protocols of non-damaging cell hypoxia have been demonstrated to significantly increase survival, proliferation and post-engraftment differentiation potential of stem cells. The best results for priming cultured stem cells against a following, otherwise lethal, ischemic stress have been obtained with brief intermittent episodes of hypoxia, or anoxia, and reoxygenation in accordance with the extraordinary protection afforded by the conventional maneuver of ischemic preconditioning in severely ischemic organs. These protocols of hypoxic preconditioning can be rather easily reproduced in a laboratory; however, more suitable pharmacological interventions inducing stem cell responses similar to those activated in hypoxia are considered among the most promising solutions for future applications in cell therapy. Here we want to offer an up-to-date review of the molecular mechanisms translating hypoxia into beneficial events for regenerative medicine. To this aim the involvement of epigenetic modifications, microRNAs, and oxidative stress, mainly activated by hypoxia inducible factors, will be discussed. Stem cell adaptation to their natural hypoxic microenvironments (niche) in healthy and neoplastic tissues will be also considered.

show abstract

Section: Discussionmentioning

confidence: 99%

Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, all the presented results were obtained using technical approaches useful for the experimental collection of proofs of principle but unlikely suitable for application in clinical protocols for regenerative medicine. Focusing on this issue, our group [62] designed a reliable innovative bioreactor, acting as a stand-alone cell culture incubator, easy to operate and effective in addressing rat MSCs seeded onto a 3D bioreabsorbable scaffold, toward a muscle phenotype via the transfer of a controlled and highly reproducible cyclic deformation. Electron microscopy, immunohistochemistry, and biochemical analysis of the pseudotissue constructs obtained after 1 week of cyclic mechanical stretch (10%; 1.6 Hz) showed cell multilayer organization and invasion of the 3D mesh of the scaffold.…”

Section: Bioreactors For Cardiac Tissue Engineeringmentioning

confidence: 99%

Mechanostimulation Protocols for Cardiac Tissue Engineering

Govoni

Muscari

Guarnieri

et al. 2013

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Owing to the inability of self-replacement by a damaged myocardium, alternative strategies to heart transplantation have been explored within the last decades and cardiac tissue engineering/regenerative medicine is among the present challenges in biomedical research. Hopefully, several studies witness the constant extension of the toolbox available to engineer a fully functional, contractile, and robust cardiac tissue using different combinations of cells, template bioscaffolds, and biophysical stimuli obtained by the use of specific bioreactors. Mechanical forces influence the growth and shape of every tissue in our body generating changes in intracellular biochemistry and gene expression. That is why bioreactors play a central role in the task of regenerating a complex tissue such as the myocardium. In the last fifteen years a large number of dynamic culture devices have been developed and many results have been collected. The aim of this brief review is to resume in a single streamlined paper the state of the art in this field.

show abstract

“…According to these preliminary results, we then investigated whether MSCs seeded onto the HYAFF®11 woven mesh scaffold could be addressed towards a muscle phenotype via the transfer of a controlled and highly-reproducible cyclic deformation [103]. The construct that was obtained after one week of mechanical stretch showed cells displaying multilayer organization, invading the 3D mesh of the scaffold, and expressing typical markers of muscle cells.…”

Section: Reviewmentioning

confidence: 99%

Hyaluronan and cardiac regeneration

et al. 2014

Self Cite

View full text Add to dashboard Cite

Hyaluronan (HA) is abundantly expressed in several human tissues and a variety of roles for HA has been highlighted. Particularly relevant for tissue repair, HA is actively produced during tissue injury, as widely evidenced in wound healing investigations. In the heart HA is involved in physiological functions, such as cardiac development during embryogenesis, and in pathological conditions including atherosclerosis and myocardial infarction. Moreover, owing to its relevant biological properties, HA has been widely used as a biomaterial for heart regeneration after a myocardial infarction. Indeed, HA and its derivatives are biodegradable and biocompatible, promote faster healing of injured tissues, and support cells in relevant processes including survival, proliferation, and differentiation. Injectable HA-based therapies for cardiovascular disease are gaining growing attention because of the benefits obtained in preclinical models of myocardial infarction. HA-based hydrogels, especially as a vehicle for stem cells, have been demonstrated to improve the process of cardiac repair by stimulating angiogenesis, reducing inflammation, and supporting local and grafted cells in their reparative functions. Solid-state HA-based scaffolds have been also investigated to produce constructs hosting mesenchymal stem cells or endothelial progenitor cells to be transplanted onto the infarcted surface of the heart. Finally, applying an ex-vivo mechanical stretching, stem cells grown in HA-based 3D scaffolds can further increase extracellular matrix production and proneness to differentiate into muscle phenotypes, thus suggesting a potential strategy to create a suitable engineered myocardial tissue for cardiac regeneration.Electronic supplementary materialThe online version of this article (doi:10.1186/s12929-014-0100-4) contains supplementary material, which is available to authorized users.

show abstract

An innovative stand-alone bioreactor for the highly reproducible transfer of cyclic mechanical stretch to stem cells cultured in a 3D scaffold

Cited by 20 publications

References 32 publications

Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine

Priming adult stem cells by hypoxic pretreatments for applications in regenerative medicine

Mechanostimulation Protocols for Cardiac Tissue Engineering

Hyaluronan and cardiac regeneration

Contact Info

Product

Resources

About