An innovative hybrid steam-microwave sterilization of palm oil fruits at atmospheric pressure

Hock, Tee K.; Chala, Girma T.; How, Ho Cheng

doi:10.1016/j.ifset.2020.102289

Cited by 13 publications

(9 citation statements)

References 18 publications

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“…This study provides in-depth insight into the impacts of microwaves on bacteria and the genomic DNA of E. coli. Because of microwaves' ability to kill bacteria, recently, microwave-based sterilization has been seen as promising due to speed and energy efficiency [39,40]. In a sterilization study, an aqueous solution of DNA was extracted from E. coli, the solution was exposed to microwave radiation (8-12 GHz), and results showed that E. coli DNA absorbs microwaves substantially [41].…”

Section: Comparison Between Automated Electrophoresis and Qubit Fluor...mentioning

confidence: 99%

Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

Pandey,

Momeni,

Pandey

2024

Gels

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The problem that this study addresses is to understand how microwave radiation is able to degrade genomic DNA of E. coli. In addition, a comparative study was made to evaluate the suitability of a high-throughput automated electrophoresis platform for quantifying the DNA degradation under microwave radiation. Overall, this study investigated the genomic DNA degradation of E. coli under microwave radiation using automated gel electrophoresis. To examine the viable organisms and degradation of genomic DNA under microwave exposure, we used three methods: (1) post-microwave exposure, where E. coli was enumerated using modified mTEC agar method using membrane filtration technique; (2) extracted genomic DNA of microwaved sample was quantified using the Qubit method; and (3) automated gel electrophoresis, the TapeStation 4200, was used to examine the bands of extracted DNA of microwaved samples. In addition, to examine the impacts of microwaves, E. coli colonies were isolated from a fecal sample (dairy cow manure), these colonies were grown overnight to prepare fresh E. coli culture, and this culture was exposed to microwave radiation for three durations: (1) 2 min; (2) 5 min; and (3) 8 min. In general, Qubit values (ng/µL) were proportional to the results of automated gel electrophoresis, TapeStation 4200, DNA integrity numbers (DINs). Samples from exposure studies (2 min, 5 min, and 8 min) showed no viable E. coli. Initial E. coli levels (at 0 min microwave exposure) were 5 × 108 CFU/mL, and the E. coli level was reduced to a non-detectable level within 2 min of microwave exposure. The relationships between Qubit and TapeStation measurements was linear, except for when the DNA level was lower than 2 ng/µL. In 8 min of microwave exposure, E. coli DNA integrity was reduced by 61.7%, and DNA concentration was reduced by 81.6%. The overall conclusion of this study is that microwave radiation had a significant impact on the genomic DNA of E. coli, and prolonged exposure of E. coli to microwaves can thus lead to a loss of genomic DNA integrity and DNA concentrations.

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Section: Comparison Between Automated Electrophoresis and Qubit Fluor...mentioning

confidence: 99%

Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

Pandey,

Momeni,

Pandey

2024

Gels

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“…The vapor pressure can be predicted by the temperature using the Antoine equation [1] [2], which reads ln c Pa Tb =− −…”

Section: Calculation Of Vapor Pressure and Temperaturementioning

confidence: 99%

“…The medium of steam sterilization is the water vapor with a certain range of steam drying degree from 97% to 100%, generally the sterilized steam is considered as a saturated steam. Many factors can affect the heat conduction of the sterilization process [1]. The saturated steam has to release a large amount of latent heat during the humid heat sterilization process to kill microbial proteins and to achieve the purpose of sterilization [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…Many factors can affect the heat conduction of the sterilization process [1]. The saturated steam has to release a large amount of latent heat during the humid heat sterilization process to kill microbial proteins and to achieve the purpose of sterilization [1][2][3][4]. However, it is unable to preserve damp products during usage and it can easily breed microorganisms again.…”

Section: Introductionmentioning

confidence: 99%

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A dry sterilization process for sterilized products after pressure steam sterilization

et al. 2021

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A dry sterilization process is designed as a post-processual technology of the pressure-steam sterilization. Three experiments are carried out to study the effects of negative pressure, pulsating air, and pulsating steam, respectively, on the process. Antoine's formula is used to predict the relationship between the vapor pressure and the temperature, which can be applied in practice.

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“…There are mainly three factors affecting the pressure steam sterilization, they are work period, work temperature and saturated steam. Nowadays, pressure steam sterilizers are widely used in medical institutions, and the saturated steam plays an important role in sterilization quality [1][2][3]. According to the international standards and China's standards, the products are sterilized and packaged after desiccation, and the packaging materials and sterilization equipment should follow what is requested by the standards (EN867-5, US-ANSI, GB 8599-1988, WS310, and GBT 19633).…”

Section: Introductionmentioning

confidence: 99%

The effect of moisture of cotton fabrics on steam sterilization

Shen

Zheng

et al. 2021

THERM SCI

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This paper is to study the effect of cotton fabric?s moisture on steam sterilization process. An experiment is designed to measure the thermal equilibrium time under different moisture concentrations. The results show that a higher moisture concentration leads to a longer thermal equilibrium time, and it does not affect the wet pack?s penetration efficiency when the moisture concentration is below the threshold value of 30%. The paper concludes that a higher humidity is unfavorable for the thermal conduction during the sterilization process.

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An innovative hybrid steam-microwave sterilization of palm oil fruits at atmospheric pressure

Cited by 13 publications

References 18 publications

Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

A dry sterilization process for sterilized products after pressure steam sterilization

The effect of moisture of cotton fabrics on steam sterilization

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