An inkjet-printed polysaccharide matrix for on-chip sample preparation in point-of-care cell counting chambers

Zhang, Xichen; Wasserberg, Dorothee; Breukers, Christian; Connell, Bridgette Janine; Schipper, P; Dalum, Joost van; Baeten, Ellen; Blink, Dorine van den; Bloem, Andries C.; Nijhuis, Monique; Wensing, Annemarie M. J.; Terstappen, Leon W.M.M.; Beck, Markus

doi:10.1039/d0ra01645d

“…Instead of solution mixing, the reagent can be pre-embedded into a microchip device. Zhang et al [69] reported a simple cell-counting microchip for whole blood analysis. Their microchip has a polysaccharide matrix layer of pre-embedded fluorophore-labeled antibodies capable of controlled reagent release.…”

Section: Microfluidic Chemical Conversion Techniquesmentioning

confidence: 99%

Recent progress of microfluidic sample preparation techniques

Xia

¹

,

Li

²

2023

J of Separation Science

2

0

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Sample preparation turns out to be one of the important procedures in complex sample analysis by affecting the accuracy, selectivity, and sensitivity of analytical results. However, the majority of the conventional sample preparation techniques still suffer from time‐consuming and labor‐intensive operations. These shortcomings can be addressed by reforming the sample preparation process in a microfluidic manner. Inheriting the advantages of rapid, high efficiency, low consumption, and easy integration, microfluidic sample preparation techniques receive increasing attention, including microfluidic phases separation, microfluidic field‐assisted extraction, microfluidic membrane separation, and microfluidic chemical conversion. This review overviews the progress of microfluidic sample preparation techniques in the last 3 years based on more than 100 references, we highlight the implementation of typical sample preparation methods in the formats of microfluidics. Furthermore, the challenges and outlooks of the application of microfluidic sample preparation techniques are discussed.

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“…The first category refers to polymer-based channels that can be fabricated using UV-curable materials. Interesting examples have been shown not only for sample distribution [113], but also in combination with novel nanomaterials to incorporate the filtration, concentration and amplification of the analyte directly within the chip before reaching the measurement point [151]. The second category refers to the fabrication of lateral-flow paper-based assays, in which the paper capillarity is exploited to guarantee the efficient flow of the sample, which is better controlled with customized hydrophilic paths printed with wax or other hydrophobic materials on paper substrates [45,152].…”

Section: Microfluidic Dispensing To Improve Repeatabilitymentioning

confidence: 99%

Electrical and Electro-Optical Biosensors

2022

0

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“…The first category refers to polymer-based channels that can be fabricated using UV-curable materials. Interesting examples have been shown not only for sample distribution [113], but also in combination with novel nanomaterials to incorporate the filtration, concentration and amplification of the analyte directly within the chip before reaching the measurement point [151]. The second category refers to the fabrication of lateral-flow paper-based assays, in which the paper capillarity is exploited to guarantee the efficient flow of the sample, which is better controlled with customized hydrophilic paths printed with wax or other hydrophobic materials on paper substrates [45,152].…”

Section: Microfluidic Dispensing To Improve Repeatabilitymentioning

confidence: 99%

Printed Electrochemical Biosensors: Opportunities and Metrological Challenges

Sardini

¹

,

Serpelloni

²

,

Tonello

³

2020

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Printed electrochemical biosensors have recently gained increasing relevance in fields ranging from basic research to home-based point-of-care. Thus, they represent a unique opportunity to enable low-cost, fast, non-invasive and/or continuous monitoring of cells and biomolecules, exploiting their electrical properties. Printing technologies represent powerful tools to combine simpler and more customizable fabrication of biosensors with high resolution, miniaturization and integration with more complex microfluidic and electronics systems. The metrological aspects of those biosensors, such as sensitivity, repeatability and stability, represent very challenging aspects that are required for the assessment of the sensor itself. This review provides an overview of the opportunities of printed electrochemical biosensors in terms of transducing principles, metrological characteristics and the enlargement of the application field. A critical discussion on metrological challenges is then provided, deepening our understanding of the most promising trends in order to overcome them: printed nanostructures to improve the limit of detection, sensitivity and repeatability; printing strategies to improve organic biosensor integration in biological environments; emerging printing methods for non-conventional substrates; microfluidic dispensing to improve repeatability. Finally, an up-to-date analysis of the most recent examples of printed electrochemical biosensors for the main classes of target analytes (live cells, nucleic acids, proteins, metabolites and electrolytes) is reported.

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An inkjet-printed polysaccharide matrix for on-chip sample preparation in point-of-care cell counting chambers

Abstract: Gellan/trehalose layers were tailored to optimize on-chip storage and release of antibodies in a simple point-of-care CD4 counting chip with excellent agreement with standard methods.

Cited by 5 publications

References 51 publications

Recent progress of microfluidic sample preparation techniques

Recent progress of microfluidic sample preparation techniques

Electrical and Electro-Optical Biosensors

Printed Electrochemical Biosensors: Opportunities and Metrological Challenges

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