An injectable thermosensitive hydrogel loaded with an ancient natural drug colchicine for myocardial repair after infarction

Chen, Yu; Shi, Jiayue; Zhang, Yaping; Miao, Jiajun; Zhao, Zhe; Jin, Xian; Liu, Liang; Yu, Lin; Shen, Chengxing; Ding, Jiandong

doi:10.1039/c9tb02523e

Cited by 63 publications

(36 citation statements)

References 63 publications

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“…Moreover, in clinical practice, colchicine has side effects, which limit its clinical use. In order to overcome the side effects of colchicine, Chen et al developed an intramyocardial delivery system of colchicine using an injectable, thermosensitive poly(lactide-co-glycolide)-poly(ethyleneglycol)poly(lactide-co-glycolide) polymer hydrogel as a vehicle for the treatment of MI while minimizing its systemic toxicity [12]. The data showed that colchicine, especially colchicine-containing nanoparticles, markedly attenuated the cardiac in ammation by inhibiting the generations of in ammatory cytokines after MI, and the colchicine-containing nanoparticles had great potential as an anti-in ammatory therapy for the treat of MI.…”

Section: Discussionmentioning

confidence: 99%

Colchicine-Containing Nanoparticles Attenuates Acute Myocardial Infarction Injury by Inhibiting Inflammation

Wang

Peng

Song

et al. 2021

Preprint

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Purpose Anti-inflammatory therapy is important for reducing myocardial injury after acute myocardial infarction (MI). New anti-inflammatory drugs and their mechanism are necessary to be explored to improve the clinical efficacy. We aimed to improve the efficacy of colchicine on attenuating MI injury by nano-drug delivery systems, and to investigate the mechanism of anti-inflammatory. Methods Colchicine-containing delivery system based on calcium carbonate nanoparticles (ColCaNPs) was synthesized. The protection against MI by ColCaNPs was evaluated using an in vivo rat model established by ligating the left anterior descending coronary artery. Macrophage polarization and the levels of inflammatory cytokines were determined using immunohistochemistry, western blot and ELISA analysis. Results ColCaNPs treatment showed about a 45% reduction in myocardial infarct size and attenuating myocardial fibrosis compared with groups without drug intervention after MI. Furthmore, ColCaNPs significantly decreased the levels of CRP, TNF-α and IL-1β in serum and the expression of proinflammatory cytokine in myocardial tissues after MI (p < 0.05). We also found that ColCaNPs notably restrained pyroptosis, and inhibited inflammatory response by modulating on M1/M2 macrophage polarization and suppressing TLR4/NFκB/NLRP3 signal pathway. Conculsion Colchicine-containing nanoparticles can protect against MI injury in a clinically relevant rat model by reducing inflammation. In addition, calcium carbonate nanoparticles can increase the cardioprotective effects of colchicine.

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Section: Discussionmentioning

confidence: 99%

Colchicine-Containing Nanoparticles Attenuates Acute Myocardial Infarction Injury by Inhibiting Inflammation

Wang

Peng

Song

et al. 2021

Preprint

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“…PEG, LA, GA, and Sn(Oct) 2 were purchased from Sigma-Aldrich (St. Louis, MO, USA). As reported in a previous study [ 43 ], the PLGA–PEG–PLGA copolymer was synthesized by ring-opening polymerization approach. Poly(ethylene glycol) (PEG) (1500 g/mol, 60 g) was dried under vacuum in a three-necked flask as the macroinitiator.…”

Section: Methodsmentioning

confidence: 99%

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Yan

Liu

et al. 2021

Stem Cell Res Ther

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Background Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Safe and non-invasive magnetic therapy combined with tissue engineering to repair cartilage may be a promising breakthrough. Methods In this study, a composite scaffold made of Hydroxyapatite-Collagen type-I (HAC) and PLGA-PEG-PLGA thermogel was produced to match the cartilage and subchondral layers in osteochondral defects, respectively. Bone marrow mesenchymal stem cells (BMSC) encapsulated in the thermogel were stimulated by an electromagnetic field (EMF). Effect of EMF on the proliferation and chondrogenic differentiation potential was evaluated in vitro. 4 mm femoral condyle defect was constructed in rabbits. The scaffolds loaded with BMSCs were implanted into the defects with or without EMF treatment. Effects of the combination treatment of the EMF and composite scaffold on rabbit osteochondral defect was detected in vivo. Results In vitro experiments showed that EMF could promote proliferation and chondrogenic differentiation of BMSCs partly by activating the PI3K/AKT/mTOR and Wnt1/LRP6/β-catenin signaling pathway. In vivo results further confirmed that the scaffold with EMF enhances the repair of osteochondral defects in rabbits, and, in particular, cartilage repair. Conclusion Hydrogel-Hydroxyapatite-Monomeric Collagen type-I scaffold with low-frequency EMF treatment has the potential to enhance osteochondral repair.

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“…As reported in a previous study [43], the PLGA-PEG-PLGA copolymer was synthesized by ring-opening polymerization approach. Poly(ethylene glycol) (PEG) was dried under vacuum in a three-necked ask as the macroinitiator.…”

Section: Preparation and Characterization Of Plga-peg-plga Thermogelmentioning

confidence: 99%

Hydrogel-Hydroxyapatite-Monomeric Collagen Type-I Scaffold with Low-Frequency Electromagnetic field Treatment Enhances Osteochondral Repair in Rabbits

Yan

Liu

Zhang

et al. 2021

Preprint

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Background: Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Methods: In this study, a composite scaffold made of Hydroxyapatite-Collagen type-I (HAC) and PLGA-PEG-PLGA thermogel was produced to match the cartilage and subchondral layers in osteochondral defects, respectively. Bone marrow mesenchymal stem cells (BMSC) encapsulated in the thermogel were stimulated by an electromagnetic field (EMF). Results: The scaffold was evaluated in rabbits, and biological characteristic of BMSCs were measured. Results showed that the scaffold with EMF enhances the repair of osteochondral defects in rabbits, and, in particular, cartilage repair. EMF could promote proliferation and chondrogenic differentiation of BMSCs partly by activating the PI3K/AKT/mTOR and Wnt1/LRP6/β-catenin pathway. Conclusion: Hydrogel-Hydroxyapatite-Monomeric Collagen type-I scaffold with low-frequency EMF treatment has the potential to enhance osteochondral repair.

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An injectable thermosensitive hydrogel loaded with an ancient natural drug colchicine for myocardial repair after infarction

Abstract: The intramyocardial injection of colchicine-loaded hydrogel system effectively promoted myocardial repair after infarction while minimizing the systemic toxicity of colchicine.

Cited by 63 publications

References 63 publications

Colchicine-Containing Nanoparticles Attenuates Acute Myocardial Infarction Injury by Inhibiting Inflammation

Colchicine-Containing Nanoparticles Attenuates Acute Myocardial Infarction Injury by Inhibiting Inflammation

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Hydrogel-Hydroxyapatite-Monomeric Collagen Type-I Scaffold with Low-Frequency Electromagnetic field Treatment Enhances Osteochondral Repair in Rabbits

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