An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in &lt;em&gt;Drosophila&lt;/em&gt;

Sandhu, Simran; Kollah, Arnavaz P.; Lewellyn, Lara; Chan, Robin F.; Grotewiel, Mike

doi:10.3791/52676-v

Cited by 2 publications

(3 citation statements)

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Section: Discussionmentioning

confidence: 99%

“…Current methods for assessing sedation time require testing large numbers of flies without readily enabling measurements on single individuals 15,16,17,18,19,20,21,22,23,24,25,26 . Many current sedation and sensitivity assays rely upon ST50 22,23,24 , the timepoint at which 50% of the flies are sedated as a result of ethanol exposure. Although obtaining the ST50 for groups of flies was not the primary motivation for developing this assay, the video recordings demonstrate higher utility compared to current methods, as the recordings can be used to ascertain the ST50 for groups of individually tested flies and to measure the percentage of flies that satisfy a given criterion (e.g., loss of postural control) at any time point.…”

Section: Discussionmentioning

confidence: 99%

“…Alternative ethanol sedation assays that do not require the elaborate inebriometer set-up allow for more convenient measurements but are still limited in throughput and generally require analyses of groups of flies rather than individuals 21,22,23,24,25 . Assessing single flies minimizes the…”

Section: Video Linkmentioning

confidence: 99%

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High-Throughput Method for Measuring Alcohol Sedation Time of Individual <em>Drosophila melanogaster</em>

Sass

MacPherson

Mackay

et al. 2020

JoVE

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Drosophila melanogaster provides an excellent model to study the genetic underpinnings of alcohol sensitivity. In contrast to studies in human populations, the Drosophila model allows strict control over genetic background, and virtually unlimited numbers of individuals of the same genotype can be reared rapidly under well-controlled environmental conditions without regulatory restrictions and at relatively low cost. Flies exposed to ethanol undergo physiological and behavioral changes that resemble human alcohol intoxication, including loss of postural control, sedation, and development of tolerance. Here, we describe a simple, low-cost, high-throughput assay for assessing alcohol sedation sensitivity in large numbers of single flies. The assay is based on video recording of single flies introduced without anesthesia in 24-well cell culture plates in a set-up that enables synchronous initiation of alcohol exposure. The system enables a single person to collect individual ethanol sedation data on as many as 2,000 flies within an 8 h work period. The assay can, in principle, be extended to assess the effects of exposure to any volatile substance and applied to measure effects of acute toxicity of volatiles on other insects, including other fly species. Video LinkThe video component of this article can be found at https://www.jove.com/video/61108/ 13,15,19,20 . Whereas inebriometer assays have led to identification of genes, genetic networks, and cellular pathways associated with alcohol sedation sensitivity and development of tolerance 12,13,14,21 , the assay is time consuming, low-throughput, and ineffective for measuring alcohol sensitivity in single flies.Alternative ethanol sedation assays that do not require the elaborate inebriometer set-up allow for more convenient measurements but are still limited in throughput and generally require analyses of groups of flies rather than individuals 21,22,23,24,25 . Assessing single flies minimizes the

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

High-Throughput Method for Measuring Alcohol Sedation Time of Individual <em>Drosophila melanogaster</em>

Sass

MacPherson

Mackay

et al. 2020

JoVE

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TBI and Tau Loss of Function Both Affect Naïve Ethanol Sensitivity in Drosophila

Hoxha,

Shrestha,

Baloch

et al. 2024

IJMS

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Traumatic brain injury (TBI) is associated with alcohol abuse and higher ethanol sensitivity later in life. Currently, it is poorly understood how ethanol sensitivity changes with time after TBI and whether there are sex-dependent differences in the relationship between TBI and ethanol sensitivity. This study uses the fruit fly Drosophila melanogaster to investigate how TBI affects alcohol sensitivity and whether the effects are sex-specific. Our results indicate that flies have a significantly higher sensitivity to the intoxicating levels of ethanol during the acute phase post-TBI, regardless of sex. The increased ethanol sensitivity decreases as time progresses; however, females take longer than males to recover from the heightened ethanol sensitivity. Dietary restriction does not improve the negative effects of alcohol post-TBI. We found that tau mutant flies exhibit a similar ethanol sensitivity to TBI flies. However, TBI increased the ethanol sensitivity of dtauKO mutants, suggesting that TBI and dtau loss of function have additive effects on ethanol sensitivity.

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An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in <em>Drosophila</em>

Cited by 2 publications

References 0 publications

High-Throughput Method for Measuring Alcohol Sedation Time of Individual <em>Drosophila melanogaster</em>

High-Throughput Method for Measuring Alcohol Sedation Time of Individual <em>Drosophila melanogaster</em>

TBI and Tau Loss of Function Both Affect Naïve Ethanol Sensitivity in Drosophila

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