An inexpensive rotary infusion pump for delivering microliter volumes of fluids to animal subjects

Kashinsky, Wayne; Rozboril, Lloyd; Robinson, Scott R.; Smotherman, William P.

doi:10.1016/0031-9384(90)90383-f

Cited by 28 publications

(20 citation statements)

References 7 publications

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“…A custom-made rotary micro-infusion pump [43] with a mounted Gilmont syringe was used to infuse fluids (0.1% saccharin or water). The rates of infusion were set equal to the mean rates of voluntary fluid consumption that animals showed in tests employing free access to these fluids.…”

Section: Methodsmentioning

confidence: 99%

The role of social isolation in ethanol effects on the preweanling rat

Kozlov

Nizhnikov

Spear

2012

Behavioural Brain Research

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The present experiments investigated the effects of acute ethanol exposure on voluntary intake of 0.1% saccharin or water as well as behavioral and nociceptive reactivity in twelve–day-old (P12) rats exposed to differing levels of isolation. The effects of ethanol emerged only during short-term social isolation (STSI) with different patterns observed in males and females and in pups exposed to saccharin or water. The 0.5 g/kg ethanol dose selectively increased saccharin intake in females, decreased rearing activity in males and attenuated isolation-induced analgesia (IIA) in all water-exposed pups. Ingestion of saccharin decreased IIA, and the 0.5 g/kg ethanol dose further reduced IIA. The 1.0 g/kg ethanol dose, administered either intragastrically or intraparentionally, also decreased IIA in P12 females, but not in P9 pups. A significant correlation between voluntary saccharin intake and baseline nociceptive reactivity was revealed in saline injected animals, saccharin intake was inversely correlated with behavioral activation and latency of reaction to noxious heat after 0.5 g/kg ethanol in females. The 0.5 g/kg ethanol dose did not affect plasma corticosterone (CORT) measured 5 hours after maternal separation or 20 minutes after ethanol injection. Female pups CORT level was inversely correlated with magnitude of IIA that accompanied the first episode of STSI (pretest isolation) 1.5–2 hours before CORT measurement. The present findings suggest that the anxiolytic properties of ethanol are responsible for enhancement of saccharin intake during STSI. Furthermore, differential reactivity of P12 males and females to STSI plays an important role in ethanol effects observed at this age.

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Section: Methodsmentioning

confidence: 99%

The role of social isolation in ethanol effects on the preweanling rat

Kozlov

Nizhnikov

Spear

2012

Behavioural Brain Research

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“…Ethanol was infused into the oral cavity by insertion of a cannula into the cheek of the pups, which was then connected to a Gilmont syringe (Barnant Co., Barrington, IL) by PE-50 polyethylene tubing. Infusion pumps were rotary microsyringe pumps controlled by an on/off switch (Kashinsky et al, 1990). The cage was maintained between 34 and 36 1C by a heating pad underneath the cage.…”

Section: Apparatusmentioning

confidence: 99%

Ethanol Acceptance Is High During Early Infancy and Becomes Still Higher After Previous Ethanol Ingestion

Sanders

Spear

2007

Alcoholism Clin & Exp Res

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Intake on P12 was increased by prior exposure to ethanol intragastrically as well as by the conventional oral route, suggesting pharmacological effects of prior ethanol exposure. Yet, the apparently greater influence of prior exposure by the oral route and the influence of prior ethanol concentration also implicate the importance of ethanol's chemosensory attributes for effects of prior ethanol exposure. The equivalent g/kg intake of ethanol in 15 or 25% solutions during the early part of the second postnatal week suggests that regulation of ethanol intake at P8 and P10 is similar to that observed previously in adults. Change in aspects of ethanol ingestion at about P6 may reflect the shift in function of the GABA system at about this age.

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“…The cannula was installed on the midline of the tongue in an anterior position (Kehoe & Blass, 1985). The intraoral infusion was delivered in a volume of 20 ml, in a pulse lasting 2 s. An automated infusion pump permitted precise ( AE 1 ml) infusions of milk into the mouth of the subject fetus without interrupting ongoing fetal activity (Kashinsky, Rozboril, Robinson, & Smotherman, 1990). …”

Section: Intraoral Cannula and Infusionmentioning

confidence: 99%