In several location, security system is becoming priority. One of the location is laboratory. There are many valuable research tools and equipment in the laboratory, so to improve the security system. Generally, the access to entry the laboratory is equipped with digital safety system. Computer Control System Laboratory at Information Technology Department of Jember State Polytechnic uses RFID-Card as access right for entrance to the laboratory. RFID-Card also function as ID-Card (printed with photo and biodata), and packaged to fit in user clothes pocket. However, using RFID gives less of flexibility. The user must remove the ID-Card and attach the card in the RFID reader box. Therefore, in this study will designed a door lock security system based on the image processing to replace the use of RFID. Later, user simply position themselves in the Pi Camera work area. Processed data from camera, the matched with image data which stored in the database. The database contains photo of laboratory members which listed on the employee IDCard. Template matching method is used as image matching method which stored in Raspberry Pi database. If the image which captured by camera is matched with the image data in the database, the controller will give entry access to the user. Based on the experimental results, template matching method has a success rate of 96%.