An Indocyanine Green‐Based Nanoprobe for <i>In Vivo</i> Detection of Cellular Senescence

Baker, Andrew G.; Hartono, Muhamad; Ou, Hui‐Ling; Popov, Andrea Bistrović; Brown, Emma L.; Joseph, James; Golinska, Monika; González‐Gualda, Estela; Macias, David; Ge, Jianfeng; Denholm, Mary; Morsli, Samir; Sanghera, Chandan; Else, Thomas R.; Greer, Heather F.; Vernet, Aude; Bohndiek, Sarah E.; Muñoz‐Espín, Daniel; Fruk, Ljiljana

doi:10.1002/anie.202404885

Cited by 3 publications

(3 citation statements)

References 70 publications

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“…(a) Synthesis and characterization: A recent study by Baker et al. revealed that the previously described methods resulted in the formation of additional side products together with ICG-Jagg; thus, ultracentrifugation is needed to separate/purify these Jagg from other side products . Moreover, their research revealed that Jagg primarily consist of ICG dimers rather than monomers.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, ICG-Jagg have been employed for in vivo monitoring of cellular senescence through fluorescence and PAI . Jagg were found to be internalized by active endocytosis and exhibited selective accumulation within the lysosomal compartment in various in vitro cellular senescence models (Figure ).…”

Section: Applications Of Icg-jaggmentioning

confidence: 99%

Section: Applications Of Icg-jaggmentioning

confidence: 99%

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Tailoring Indocyanine Green J-Aggregates for Imaging, Cancer Phototherapy, and Drug Delivery: A Review

Kumari,

Arora,

Pan

et al. 2024

ACS Appl. Bio Mater.

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Indocyanine green J-aggregates (ICG-Jagg) have emerged as a significant subject of interest in biomedical applications due to their unique optical properties, tunable size, and excellent biocompatibility. This comprehensive review aims to provide an in-depth exploration of ICG-Jagg, with a focus on elucidating the diverse facets of their preparation and the factors that influence the preparation process. Additionally, the review discusses their applications in biomedical diagnostics, such as imaging and contrast agents, as well as their utilization in drug delivery and various phototherapeutic interventions.

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Section: Discussionmentioning

confidence: 99%

Section: Applications Of Icg-jaggmentioning

confidence: 99%

See 1 more Smart Citation

Tailoring Indocyanine Green J-Aggregates for Imaging, Cancer Phototherapy, and Drug Delivery: A Review

Kumari,

Arora,

Pan

et al. 2024

ACS Appl. Bio Mater.

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A second generation of senotherapies: the development of targeted senolytics, senoblockers and senoreversers for healthy ageing

Dhokia,

Albati,

Smith

et al. 2024

Biochemical Society Transactions

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Cellular senescence, a form of terminal cell cycle arrest, is as a key driver of organismal ageing and an important factor in age-related diseases. Insights into the senescent phenotype have led to the development of novel therapeutic strategies, collectively known as senotherapies, that aim to ameliorate the detrimental effects of senescent cell accumulation in tissues. The senotherapeutic field has rapidly evolved over the past decade, with clinical translation of the first drugs discovered currently underway. What began as the straightforward removal of senescent cells using repurposed compounds, which were given the name of senolytics, has grown into an expanding field that uses different state of the art approaches to achieve the goal of preventing the build-up of senescent cells in the body. Here, we summarize the emergence of a new generation of senotherapies, based on improving the efficacy and safety of the original senolytics by making them targeted, but also branching out into drugs that prevent senescence (senoblockers) or revert it (senoreversers).The use of nanotechnology, specific antibodies, cell-based approaches and restored immunosurveillance is likely to revolutionize the field of senotherapies in the near future, hopefully allowing it to realize its full clinical potential.

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Photoacoustic Polydopamine-Indocyanine Green (PDA-ICG) Nanoprobe for Detection of Senescent Cells

Hartono,

Baker,

Else

et al. 2024

Preprint

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Cellular senescence is considered an important tumour suppression mechanism in response to damage and oncogenic stress in early lesions. However, when senescent cells are not immune-cleared and persist in the tumour microenvironment, they can drive a variety of tumour-promoting activities, including cancer initiation, progression, and metastasis. Additionally, there is compelling evidence demonstrating a direct connection between chemo(radio)therapy-induced senescence and the development of drug resistance and cancer recurrence. Therefore, detection of senescent cells in tissues holds great promise for predicting cancer occurrence earlier, assessing tumour progression, aiding patient stratification and prognosis, and informing about the efficacy of potential senotherapies. However, effective detection of senescent cells is limited by lack of biomarkers and readout strategies suitable for in vivo clinical imaging. To this end, a nanoprobe composed of biocompatible polydopamine (PDA) nanoparticle doped with FDA-approved indocyanine green (ICG) dye, namely PDA-ICG, was designed as a contrast agent for senescence detection using photoacoustic imaging (PAI). In an in vitro model of chemotherapy-induced senescence, PDA-ICG nanoprobe showed an elevated uptake in senescent cells relative to cancer cells. In addition to its improved photostability, 2.5-fold enhancement in photoacoustic signal relative to ICG was observed. Collectively, the results indicate that the PDA-ICG nanoprobe has the potential to be used as a contrast agent for senescence detection of chemotherapy-induced senescence using PAI.

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An Indocyanine Green‐Based Nanoprobe for In Vivo Detection of Cellular Senescence

Cited by 3 publications

References 70 publications

Tailoring Indocyanine Green J-Aggregates for Imaging, Cancer Phototherapy, and Drug Delivery: A Review

Tailoring Indocyanine Green J-Aggregates for Imaging, Cancer Phototherapy, and Drug Delivery: A Review

A second generation of senotherapies: the development of targeted senolytics, senoblockers and senoreversers for healthy ageing

Photoacoustic Polydopamine-Indocyanine Green (PDA-ICG) Nanoprobe for Detection of Senescent Cells

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