An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall

Costa, Mónica; Pio, Luís; Bule, Pedro; Cardoso, Vânia; Alfaia, Cristina M.; Coelho, Diogo; Brás, Joana L. A.; Fontes, Carlos M. G. A.; Prates, José A. M.

doi:10.1038/s41598-021-89278-1

Cited by 15 publications

(19 citation statements)

References 58 publications

(92 reference statements)

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“…They were housed individually in metabolic crates, equipped with individual heating lamps and nipple drinkers. The piglets were then randomly allocated to each of the four experimental groups ( n = 10): control (maize, wheat, and soybean meal-based diet), LA (10% L. digitata , replacing the control diet), LAR (LA + CAZyme-0.005% Rovabio ® Excel AP from Adisseo (Antony, France)) and LAL (LA + CAZyme-0.01% pre-selected alginate lyase as described by Costa et al [ 19 ]). Experimental diet composition is presented in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

“…The commercially available enzyme mixture Rovabio ® Excel AP was designed for use in cereal-based diets to degrade non-starch polysaccharides and has been used in microalgae-containing diets [ 6 , 7 , 18 ]. Alginate lyase, an alginate-degrading enzyme, has been reported to degrade the cell walls of L. digitata in vitro [ 19 ], being a putative candidate for in vivo studies.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and Nutritional Value of Meat

Ribeiro

Alfaia

Pestana

et al. 2022

Foods

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Laminaria digitata is a novel feedstuff that can be used in pig diets to replace conventional feedstuffs. However, its resilient cell wall can prevent the monogastric digestive system from accessing intracellular nutrients. Carbohydrate-active enzyme (CAZyme) supplementation is a putative solution for this problem, degrading the cell wall during digestion. The objective of this work was to evaluate the effect of 10% L. digitata feed inclusion and CAZyme supplementation on the meat quality and nutritional value of weaned piglets. Forty weaned piglets were randomly allocated to four experimental groups (n = 10): control, LA (10% L. digitata, replacing the control diet), LAR (LA + CAZyme (0.005% Rovabio® Excel AP)) and LAL (LA + CAZyme (0.01% alginate lyase)) and the trial lasted for two weeks. The diets had no effect on any zootechnical parameters measured (p > 0.05) and meat quality traits, except for the pH measured 24 h post-mortem, which was higher in LAL compared to LA (p = 0.016). Piglets fed with seaweed had a significantly lower n-6/n-3 PUFA ratio compared to control, to which the higher accumulation of C20:5n-3 (p = 0.001) and C18:4n-3 (p < 0.0001) contributed. In addition, meat of seaweed-fed piglets was enriched with bromine (Br, p < 0.001) and iodine (I, p < 0.001) and depicted a higher oxidative stability. This study demonstrates that the nutritional value of piglets’ meat could be improved by the dietary incorporation of L. digitata, regardless of CAZyme supplementation, without negatively affecting growth performance in the post-weaning stage.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and Nutritional Value of Meat

Ribeiro

Alfaia

Pestana

et al. 2022

Foods

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“…In the pursuit of degrading macroalgae cell wall, it has been already shown that the in vitro addition of a single CAZyme, i.e. alginate lyase , among other benefits, improves lipid yield extraction and sugar recovery in Laminaria digitata ( L. digitata ), a typical example of a brown seaweed [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Combined effects of dietary Laminaria digitata with alginate lyase on plasma metabolites and hepatic lipid, pigment and mineral composition of broilers

et al. 2022

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Background The Laminaria digitata is an abundant macroalga and a sustainable feedstock for poultry nutrition. L. digitata is a good source of essential amino acids, carbohydrates and vitamins, including A, D, E, and K, as well as triacylglycerols and minerals, in particular iron and calcium. However, the few studies available in the literature with broilers document the application of this macroalga as a dietary supplement rather than a feed ingredient. No study has addressed up until now the effects of a high-level incorporation (> 2% in the diet) of L. digitata on plasma biochemical markers and hepatic lipid composition, as well as minerals and pigments profile in the liver of broilers. Our experimental design included one hundred and twenty Ross 308 male birds contained in 40 wired-floor cages and distributed to the following diets at 22 days of age (n = 10) for 15 days: 1) a corn-soybean basal diet (Control); 2) the basal diet plus 15% of L. digitata (LA); 3) the basal diet plus 15% of L. digitata with 0.005% of Rovabio® Excel AP (LAR); and 4) the basal diet plus 15% of L. digitata with 0.01% of the recombinant CAZyme, alginate lyase (LAE). Results L. digitata compromised birds’ growth performance by causing a reduction in final body weight. It was found an increase in hepatic n-3 and n-6 fatty acids, in particular C18:2n-6, C18:3n-6, C20:4n-6, C20:5n-3, C22:5n-3 and C22:6n-3 with the addition of the macroalga, with or without feed enzymes, to the broiler diets. Also, the beneficial C18:3n-3 fatty acid was increased by combining L. digitata and commercial Rovabio® Excel AP compared to the control diet. The sum of SFA, MUFA and the n-6/n-3 PUFA ratio were decreased by L. digitata, regardless the addition of exogenous enzymes. β-carotene was enhanced by L. digitata, individually or combined with CAZymes, being also responsible for a positive increase in total pigments. Macrominerals, in particular phosphorous and sulphur, were increased in the liver of broilers fed L. digitata individually relative to the control. For microminerals, copper, iron and the correspondent sum were consistently elevated in the liver of broilers fed L. digitata, individually or combined with exogenous CAZymes. The powerful discriminant analysis tool based on the hepatic characterization revealed a good separation between the control group and L. digitata diets but failed to discriminate the addition of feed enzymes. Conclusions Overall, this study highlights the value of L. digitata as a feed ingredient for the poultry industry. Moreover, we can conclude that the effect of L. digitata overpowers the effect of feed enzymes, both the Rovabio® Excel AP and the alginate lyase. Having in mind the negative effects observed on birds’ performance, our main recommendation at this stage is to restraint L. digitata incorporation level in forthcoming nutritional studies.

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“…Endoguluronate lyase from Zobellia galactanivorans DsiJT and Aly7D from Saccharophagus degradans 2-40, which contain CBM32, could effectively destroy the Laminaria digitata recalcitrant cell wall, while other alginate lyases without CBM32, such as PA1167 from Pseudomonas aeruginosa PAO1 and A1m from Agarivorans sp. JAM-A1m had no effect (Costa et al, 2021). Therefore, we speculate that CBM32 plays a significant role in the degradation of insoluble alginate.…”

Section: Discussionmentioning

confidence: 78%

The Function of CBM32 in Alginate Lyase VxAly7B on the Activity on Both Soluble Sodium Alginate and Alginate Gel

et al. 2022

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Carbohydrate-binding modules (CBMs), as an important auxiliary module, play a key role in degrading soluble alginate by alginate lyase, but the function on alginate gel has not been elucidated. Recently, we reported alginate lyase VxAly7B containing a CBM32 and a polysaccharide lyase family 7 (PL7). To investigate the specific function of CBM32, we characterized the full-length alginate lyase VxAly7B (VxAly7B-FL) and truncated mutants VxAly7B-CM (PL7) and VxAly7B-CBM (CBM32). Both VxAly7B-FL and native VxAly7B can spontaneously cleavage between CBM32 and PL7. The substrate-binding capacity and activity of VxAly7B-CM to soluble alginate were 0.86- and 1.97-fold those of VxAly7B-FL, respectively. Moreover, CBM32 could accelerate the expansion and cleavage of alginate gel beads, and the degradation rate of VxAly7B-FL to alginate gel beads was threefold that of VxAly7B-CM. Results showed that CBM32 is not conducive to the degradation of soluble alginate by VxAly7B but is helpful for binding and degradation of insoluble alginate gel. This study provides new insights into the function of CBM32 on alginate gel, which may inspire the application strategy of CBMs in insoluble substrates.

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An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall

Cited by 15 publications

References 58 publications

Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and Nutritional Value of Meat

Influence of Feeding Weaned Piglets with Laminaria digitata on the Quality and Nutritional Value of Meat

Combined effects of dietary Laminaria digitata with alginate lyase on plasma metabolites and hepatic lipid, pigment and mineral composition of broilers

The Function of CBM32 in Alginate Lyase VxAly7B on the Activity on Both Soluble Sodium Alginate and Alginate Gel

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