An indirect competitive enzyme-linked immunosorbent assay for determination of norfloxacin in waters using a specific polyclonal antibody

The combinatorial molecularly imprinted polymer was prepared by using norfloxacin-Zn (II) complex as a template molecule, amethacrylic acid as a functional monomer in this article. The imprinted sites of the polymer were characterized by Infrared spectroscopy. The analysis results indicated that the metal coordination complex was formed by the 3-carbonxyl group and 4-carbonyl of the norfloxacin and carboxyl of the a-methacrylic acid and the hydrogan bonds was combined by the 3-hydroxy of norfloxacin and carboxyl of another a-methacrylic acid. Besides the selective recognition experiments showed that polymer exhibited significant adsorption selectivity for norfloxacin.

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“…Dissociation coefficient (K) and separation factor (a) were calculated based on the following Eqs. (2) and (3) [17] , respectively.…”

Section: Study Of Selectivity Adsorption To Different Substratesmentioning

confidence: 99%

Preparation of Molecularly Imprinted Polymer Based on Combinatorial Imprinted for Recognition of Norfloxacin

Huangfu

Wang

2013

Polymer-Plastics Technology and Engineering

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“…Comparison of CEIA-LIF, ELISA, and HPLC-MS/MS for the Determination of Norfloxacin The determination of norfloxacin by traditional enzymelinked immunosorbent assay (ELISA) (Cui et al 2011) has been described previously. Now, in order to exhibit the merits of CEIA-LIF, the determination of norfloxacin by HPLC-MS/ MS was also investigated, and the comparison of CEIA-LIF, ELISA, and HPLC-MS/MS was concluded in Table 2.…”

Section: Recovery Studies Of Spiked Food Samplesmentioning

confidence: 99%

“…Currently, IA has been proven to be a sensitive and reliable method, in which, except for the traditional immunoanalytical methods (Cui et al 2011;Sheng et al 2011;Liu et al 2013;Fan et al 2012), surface plasmon resonance biosensor immunoassay (Huet et al 2009;Marchesini et al 2007) and immunoaffinity chromatographic (Zhao et al 2009) methods are also available for the determination of norfloxacin in food samples. Unfortunately, most of them suffer from limitations, such as tedious procedures, high solvent and sample consumption, and low sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

et al. 2014

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A simple and sensitive capillary electrophoresis immunoassay method with laser-induced fluorescence detector was developed for the determination of norfloxacin in food samples. The assay was based on the competitive immunoreaction between fluorescein isothiocyanate-labeled norfloxacin tracer and free norfloxacin with a limited amount of antinorfloxacin antibody. In this study, the experimental parameters affecting the norfloxacin determination were systematically optimized including incubation time, buffer pH and concentration, and separation voltage. Under the optimal conditions, norfloxacin could be accurately quantified within 3 min using Na 2 B 4 O 7 /NaH 2 PO 4 buffer (30 mmol/L, pH 8.0) for background electrolyte and 25 kV for the separation voltage. The linear range of the method was 0.08-50 μg/L with a detection limit of 0.005 μg/L. The relative standard deviations for migration time and peak area of the immunocomplex were 0.17 % (intraday) and 3.46 % (intraday), respectively. The proposed method has been further applied to the determination of norfloxacin in milk, chicken, pork, and fish samples, exhibiting satisfactory recovery and sensitivity. These results indicated that the proposed method might serve as an efficient tool for the selective determination of norfloxacin in food samples.

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“…Current detection methods include enzyme-linked immunosorbent assay (ELISA) (Aga et al 2003;Cui et al 2011), capillary electrophoresis (CE) (García-Campaña et al 2009), and liquid chromatography (LC) (Chen et al 2014;Dafale et al 2013;Opriş et al 2013;Sacher et al 2001). ELISA is a rapid immunochemical test method, which is mainly used as a preliminary quantitative analysis method to screen antibiotic residues in aqueous samples.…”

Section: Introductionmentioning

confidence: 98%

Ultra-high performance liquid chromatography-electrospray tandem mass spectrometry for the analysis of antibiotic residues in environmental waters

Xue

Liu

2015

Environ Sci Pollut Res

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An optimized solid-phase extraction (SPE) and ultra-high performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-MS/MS) method was developed for the effective analysis of 35 antibiotics including sulfonamides (SAs), quinolones (QLs), tetracyclines (TCs), macrolides (MALs), lincomycin (LIN), and chloramphenicol (CAP). The addition of 0.1% formic acid to the mobile phase was favorable for the formation of [M + H](+) and the enhancement in the detection signals, but using ammonium formate decreased [M + H](+) with a corresponding reduction in the response of CAP. The optimal pH range for the SPE was 4.5 ∼ 5.0 with 6 mL aqueous ammonia/methanol (5/95, v/v) as the optimized eluent. An internal standard (IS) was selected for each type of analytes based on similarities in classification and retention time. The detection was completed in less than 10 min and was excellent with method detection limits (MDL) of 0.29 ∼ 4.03 ng/L. The recoveries of the antibiotics in samples from ultrapure water and groundwater were 67.13 ∼ 93.00% and 68.91 ∼ 92.67%, respectively. The antibiotics in samples collected from wastewater, surface water, and groundwater were also effectively detected. This newly developed method has the advantages of short detection times, small sample consumption, excellent reproducibility, and high sensitivity. This provides a reliable and promising technique for the simultaneous detection and monitoring of various residual antibiotics in aqueous environmental samples.

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An indirect competitive enzyme-linked immunosorbent assay for determination of norfloxacin in waters using a specific polyclonal antibody

Cited by 45 publications

References 28 publications

Preparation of Molecularly Imprinted Polymer Based on Combinatorial Imprinted for Recognition of Norfloxacin

Preparation of Molecularly Imprinted Polymer Based on Combinatorial Imprinted for Recognition of Norfloxacin

Determination of Norfloxacin in Food by Capillary Electrophoresis Immunoassay with Laser-Induced Fluorescence Detector

Ultra-high performance liquid chromatography-electrospray tandem mass spectrometry for the analysis of antibiotic residues in environmental waters

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